LOCUS       sequence-413001-       10011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pMx/ATP8B4-HA
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5587..5611)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5643..5669
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5838..5858)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5843..6172
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6023..6158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6529..7323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6583..6602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7193..7212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7516..7985
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8115..8132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8286..8874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8366..8385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9038..9829,9830..9898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9661..9680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9899..10003)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ctctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccaccatg ttctgcagtg aaaagaaatt gcgtgaagtg gaacggatag
     2041 tgaaagccaa tgaccgtgaa tataatgaaa agttccagta tgcggataat cgtatccaca
     2101 catcgaaata taatattctc accttcttgc caattaattt atttgaacag ttccaaagag
     2161 tggcaaatgc ctattttctt tgccttctga ttttacagct aattccagaa atttcctcct
     2221 tgacctggtt taccaccatt gtgcctttgg tcctggtgat aactatgaca gctgtcaaag
     2281 atgccacaga tgactatttt cgccacaaga gtgataatca agtgaataat cggcagtctg
     2341 aagtgctcat caacagcaaa ctgcagaatg aaaaatggat gaatgtcaaa gtgggagaca
     2401 tcattaaatt agaaaataac caatttgttg ctgctgattt acttctccta tcaagtagtg
     2461 agccacatgg tctctgttat gttgaaactg ctgagcttga tggggaaacg aacctaaaag
     2521 tccgccatgc actatcagtt acttcagaac ttggagcaga tatcagcaga cttgcagggt
     2581 ttgatgggat tgttgtctgt gaggtgccta acaacaagtt agataaattc atgggaatcc
     2641 tttcttggaa agacagcaag cattccctca acaatgagaa gataatcctg agaggctgca
     2701 tcctgagaaa taccagctgg tgttttggaa tggttatttt tgcaggtcct gacactaaac
     2761 taatgcagaa tagtggtaag acaaagttta aaaggacaag cattgataga ttgatgaata
     2821 ctctagtact atggattttt gggtttctga tatgcttggg aattattctt gcaataggaa
     2881 attcaatctg ggagagtcaa actggggacc aattcagaac tttcctcttt tggaatgaag
     2941 gagagaagag ctctgtgttc tccggattct taacattctg gtcatatatt attattctca
     3001 atacagttgt acccatttcc ttatatgtga gtgtggaagt aattcgtcta ggacacagtt
     3061 attttataaa ctgggaccgg aagatgtatt attctcgaaa agcaatacct gcagtggctc
     3121 gaacgaccac gctcaatgag gaactggggc agattgagta cattttctcc gacaaaacgg
     3181 gtaccctcac tcaaaacatc atgaccttta aaagatgttc cattaatggg agaatctatg
     3241 gtgaagtaca tgatgacctg gatcagaaga cagaaataac tcaggaaaaa gagcctgtgg
     3301 atttctcagt caaatctcaa gcggatagag aatttcagtt ctttgaccac catctgatgg
     3361 aatccattaa aatgggtgat cccaaagttc atgaattcct taggttactt gctctctgcc
     3421 acactgtaat gtcagaagag aatagcgcag gagagctgat ttaccaagtt cagtcacctg
     3481 atgaaggggc tctagtgact gccgctagaa attttgggtt catttttaaa tcccggaccc
     3541 cagagaccat aacaatagaa gaattgggaa cactagttac ttatcaatta cttgcctttt
     3601 tggatttcaa caacaccaga aaaaggatgt ctgtcatagt tcgaaaccca gaaggacaga
     3661 taaagcttta ttccaaagga gcagatacta ttctgtttga aaaacttcat ccttccaatg
     3721 aagtcctttt gtctttgacg tcagaccacc tcagtgaatt tgcaggggaa ggccttcgga
     3781 ccttggccat cgcatacaga gacctggatg acaagtactt taaagagtgg cataagatgc
     3841 ttgaagatgc gaatgctgcc acagaagaga gggatgaacg aatagctgag ctatatgaag
     3901 aaattgaaag agatttgatg ctactaggtg ccactgctgt agaagataag ttacaggagg
     3961 gtgttattga aacagttaca agtttatcac tagccaatat taagatctgg gtcctaacag
     4021 gagacaaaca agaaactgcc atcaacatcg gttatgcctg caacatgctg actgacgaca
     4081 tgaatgatgt gtttgtgata gcagggaata atgctgtgga agtgagagaa gaactcagga
     4141 aagcaaaaca aaatttgttt ggacaaaaca gaaatttttc caatggccat gtagtttgtg
     4201 aaaaaaagca gcagctggag ttggattcta ttgtagaaga aaccataaca ggagattatg
     4261 ccttaatcat aaatggccac agtttggctc atgccctaga aagtgatgtc aagaatgatc
     4321 tcctagaact tgcttgcatg tgtaagactg taatttgctg cagggtcact ccactccaga
     4381 aagcccaagt ggtagagctg gtgaagaagt acagaaatgc tgttactttg gccattggtg
     4441 atggagccaa tgatgtcagc atgattaaaa gtgctcacat tggtgttggc atcagcggcc
     4501 aggaaggatt gcaagcagtc ttagccagcg actattcatt tgcacagttt agatatctcc
     4561 aaaggcttct ccttgttcat ggaaggtggt cttatttccg aatgtgcaaa ttcttatgct
     4621 atttcttcta taagaatttt gcatttacac ttgtgcattt ctggtttggt ttcttctgtg
     4681 gtttctcagc ccagactgtt tatgaccagt ggttcatcac cctttttaac attgtttaca
     4741 catcactgcc tgttttagcc atggggattt ttgaccagga tgtgagtgac cagaacagcg
     4801 tggactgtcc ccagctctac aaaccaggac agctgaatct gctttttaac aagcgtaaat
     4861 ttttcatttg cgtgttgcat ggaatctaca cctcattagt ccttttcttc atcccctatg
     4921 gggcctttta caacgtggct ggagaagatg ggcaacatat tgctgactac cagtcctttg
     4981 cagttaccat ggccacatct ttggtcattg tggtcagtgt gcagatagcc ttggatacca
     5041 gttactggac tttcattaat cacgtcttca tctgggggag cattgccatt tatttctcca
     5101 ttttatttac aatgcacagt aatggcatct ttggcatctt cccaaaccag tttccatttg
     5161 ttggtaatgc acgacattcc ctgacccaga agtgcatctg gcttgtaatt ctcttaacaa
     5221 cagtggcttc agttatgcca gtggtggcat tcagattttt gaaggtggat ttatacccaa
     5281 ccctgagtga tcagatccgc cggtggcaga aggctcaaaa gaaggcaagg cctccaagta
     5341 gccgaaggcc tcggacccgc aggtcaagct caagaaggtc tggatatgct tttgctcacc
     5401 aagaaggcta tggagagctt atcacatctg gaaaaaatat gcgagctaaa aatccacccc
     5461 caacatcagg gctggaaaag acacattata atagcactag ctggattgaa aatttatgta
     5521 agaaaaccac agacaccgtg agcagcttta gccaggataa aacagtgaaa ctctcgagat
     5581 atctagaccc agctttcttg tacaaagtgg ttgatctaga gggcccgcgg ttcgaactcg
     5641 agtatccata cgatgtacca gactacgcag agatccacta gttcaattcc agctgagcgc
     5701 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
     5761 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaactcga gcggccgcca
     5821 gcacagtggt cgactgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6001 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6061 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6121 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttca
     6181 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     6241 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     6301 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     6361 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     6421 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     6481 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     6541 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6601 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6661 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6721 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6781 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6841 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6901 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6961 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     7021 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     7081 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     7141 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     7201 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     7261 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7321 tgagcgggac tctggggttc gcatcgtcga cgataaaata aaagatttta tttagtctcc
     7381 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     7441 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     7501 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     7561 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     7621 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     7681 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     7741 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     7801 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgattg
     7861 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     7921 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     7981 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     8041 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8101 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8161 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8221 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8281 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8341 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8401 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8461 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8521 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8581 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8641 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8701 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8761 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8821 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8881 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8941 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat
     9001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt c
//
