LOCUS       sequence-413011-        8504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat alpha2delta-1 calcium channel auxillary subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8504)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 8504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: cacna2d1 pCAGGS
FEATURES             Location/Qualifiers
     source          1..8504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5525..5544)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5590..5645
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5644..5663)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6006..6022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6006..6022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6019..6041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6030..6046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6054..6060,6061..6078,6079..6084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6099..6120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6179..6374
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6225..6360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6380..6514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6417..6436)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6471..6490
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6582..6599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6753..7341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6833..6852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7512..8303,8304..8372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8135..8154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8373..8477)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggsgcg ccaggggggg gggggggggg ggaggggggg
      541 gggggggsga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccggggr gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ctgcagatcg
     1741 cgaagatggc tgctggctgc ctgctggcct tgactctgac acttttccaa tcttggctga
     1801 tcggcccctc gagcgaggag cccttccctt cgcccgtcac tatcaagtca tgggtggata
     1861 aaatgcagga agaccttgtc acactggcaa agacagcaag tggagtgact cagcttgctg
     1921 acatttatga aaaataccaa gatttgtata ctgtggagcc aaataatgca cgccaactgg
     1981 ttgaaattgc agctagagac attgagaaac ttctgagcaa cagatctaaa gccctggtgc
     2041 gcctggctat ggaagcagag aaagtccaag ccgcccacca atggagggaa gattttgcaa
     2101 gcaacgaagt tgtctactac aacgctaaag acgatcttga tcctgaaaga aatgagagtg
     2161 agtcaggcag ccaacggatt aaacctgttt tcatcgaaga cgctaatttt ggacgccaga
     2221 tatcctatca acatgcagcg gtccatattc ccacggacat ctatgagggc tcaaccatag
     2281 tgttaaacga actcaactgg acaagtgcct tagatgaagt tttcaaaaga aatcgagatg
     2341 aagaccctac attgctgtgg caagtgtttg gcagcgccac cggcctggcc cgatattatc
     2401 cagcttctcc gtgggtggat aacagcagaa ctccaaacaa gatcgatctc tacgatgtac
     2461 gcagaagacc atggtacatc cagggagctg catcccccaa agacatgctc attctggtgg
     2521 acgtgagtgg aagtgtgagc ggattgactc tgaaactcat ccggacatct gtctctgaga
     2581 tgttagaaac cctttccgat gatgatttcg tgaatgtagc ttcatttaac agcaatgctc
     2641 aggatgtgag ttgtttccag cacctggttc aagcgaatgt aaggaacaag aaggtgctga
     2701 aggatgcggt gaataacatc acagcaaagg ggataacaga ttacaagaaa ggctttagct
     2761 tcgcgtttga acagctactt aattataatg tttccagagc caattgtaat aagatcatca
     2821 tgttattcac ggatggagga gaagagagag cccaggagat atttgccaag tacaataaag
     2881 acaaaaaagt ccgcgtgttt acattttctg ttggtcaaca taattatgac agaggaccta
     2941 ttcagtggat ggcttgtgaa aacaaaggtt attattatga gattccctct attggtgcaa
     3001 taagaatcaa tactcaggaa tatctagatg ttctgggaag accaatggtt ttagccgggg
     3061 acaaagcaaa gcaagtccag tggacaaatg tgtatttgga tgctctggag ctgggacttg
     3121 tcattactgg aactctaccg gtcttcaacg tcactggcca gtctgaaaat aagactaact
     3181 tgaagaacca gttgattctt ggtgtgatgg gcgttgatgt gtctctggaa gatattaaga
     3241 gattgacacc acgttttaca ctgtgcccca acggttacta ttttgcaatc gatcctaatg
     3301 gttatgtctt actgcatcca aatcttcagc caaagaaccc caaatctcag gagccggtca
     3361 cactggattt tctcgatgct gagttagaga atgatattaa agtggagatt cgaaataaaa
     3421 tgatagacgg agaaagtgga gaaaaaacat tcagaactct ggtcaaatct caagatgaga
     3481 gatatattga caaaggaaat cgaacataca catggacacc tgtcaatggc acagattaca
     3541 gtttggcctt ggtattgcca acctacagtt tttactatat aaaagccaaa atagaagaga
     3601 caataactca ggccagatca aaaaagggga aaatgaagga ttcagaaacc ctgaagccag
     3661 acaattttga agaatctggc tacactttca tagcaccaag ggaatactgc aatgacctga
     3721 aaccttcaga taataacact gaatttcttt taaatttcaa tgaatttatt gataggaaaa
     3781 ctccaaacaa cccttcctgt aatacagatt tgattaatag aatcttgctg gatgcgggtt
     3841 ttacaaatga acttgtccaa aattattgga gtaaacagaa aaatatcaaa ggagtgaagg
     3901 cacgctttgt ggtgactgat ggcggaatta caagagttta ccccaaagag gctggagaaa
     3961 actggcaaga gaacccagag acgtatgagg acagcttcta caaacggagc ctagataacg
     4021 ataactacgt tttcactgcg ccctacttca acaaaagtgg acctggtgcc tatgaatctg
     4081 gaattatggt aagcaaagct gtagaactgt acatccaagg aaaacttctt aagcctgcag
     4141 ttgtgggaat taaaattgac gtaaactcct ggatagaaaa ttttaccaaa acttcaatca
     4201 gggatccgtg tgctggtcca gtttgtgact gcaaaagaaa cagtgatgta atggactgtg
     4261 tcattctaga tgatggtgga tttcttctga tggccaatca tgatgattac actaatcaga
     4321 ttggacgttt ttttggagag attgacccga gcatgatgag acacctggtt aatatatcac
     4381 tttatgcatt caataaatca tatgactatc agtctgtgtg tgatccaggg gcagcaccaa
     4441 agcaaggagc aggacatcgc tcagcctatg tgccatcaat tacagacata ctccagattg
     4501 gctggtgggc cactgctgcc gcctggtcta ttctccagca gctactcttg agtttgacat
     4561 ttccacggct ccttgaggca gttgaaatgg aagaggatga cttcacagct tccctgtcta
     4621 agcagagctg catcacagaa caaactcagt acttcttcaa gaacgatact aaatcattca
     4681 gtggtttact ggactgtgga aactgttcca ggatctttca tgttgagaag cttatgaaca
     4741 ccaacctagt gttcataatg gtggagagca agggaacatg tccgtgtgac acgcggctgc
     4801 tcatgcaagc agaacagact tctgatggtc cagatccttg cgacatggtt aagcagccca
     4861 gataccgaaa aggacctgat gtctgctttg ataacaatgt gctggaggat tataccgact
     4921 gtggtggtgt ttctggatta aacccttcct tatggtctat ctttggactc caatttatac
     4981 tcctttggct ggtatctggc agcagacact atctatggtg accctctaaa cccatgatct
     5041 gcaacttaat tccatgccct gccaaaacat gagccctcag ttacattaaa atagagcccg
     5101 ttgaagaagc tccacagaac cttagctggg cctctaccct gatgtaactg tgaggcttag
     5161 attcctgacc atcgctggct gcatgtcagg gtgttggaaa ctgaaactcg tgtgaacatg
     5221 ctgcatcatc tgtgtccaac atcaaagcaa agctctactc attctctgtg ggaggtttgg
     5281 gagttcgctg ttgcactgtt ggagattacg tggaaaaggc tcccccacgc aggccttatg
     5341 aaaagaagca gtcttgactt tgacctagca tttcaacctg ctgcagtttt accaagccga
     5401 attcgcatgc ggccgctagc tcgaggtacc gatatcgagc tcaattcact cctcaggtgc
     5461 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     5521 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     5581 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     5641 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     5701 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     5761 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     5821 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     5881 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     5941 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
     6001 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6061 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6121 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     6181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6301 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6361 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     6421 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6481 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     6541 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7381 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7501 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7561 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7621 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7681 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7741 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7801 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7861 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7921 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7981 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8041 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8101 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8161 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8221 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8281 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8341 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8401 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8461 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgg
//
