LOCUS       sequence-413012-        9831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP11C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pMx/ATP11C-HA
FEATURES             Location/Qualifiers
     source          1..9831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5073..5084)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     protein_bind    complement(5407..5431)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5463..5489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5658..5678)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5663..5992
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5843..5978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6349..7143
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6403..6422)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7013..7032
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7336..7805
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7935..7952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8106..8694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8186..8205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8858..9649,9650..9718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9481..9500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9719..9823)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ctctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccaccatg cagatggtcc catctctccc tccagcctct gagtgtgctg
     2041 gagaagagaa acgagttggc acacgcacag tgtttgttgg caatcatcca gtttcggaaa
     2101 cagaagctta cattgcacaa agattttgtg ataatagaat agtctcatct aagtatacac
     2161 tttggaattt tctcccaaag aatctgtttg aacagtttag aagaattgca aatttttatt
     2221 ttctcataat cttccttgta caggtcacag tagacacacc aactagccca gttaccagtg
     2281 gacttccact tttctttgtt ataactgtta cagccatcaa gcagggatat gaggattggc
     2341 tgagacacag agctgacaat gaagtcaaca aaagcactgt ttacattatt gaaaatgcaa
     2401 agcgagtgag aaaagaaagt gaaaaaatca aggttggtga tgtagtagaa gtacaggcag
     2461 atgaaacctt tccctgtgat cttattcttc tatcatcttg caccactgat ggaacctgtt
     2521 atgtcactac agccagtctt gatggggaat ccaattgcaa gacacattat gcagtacgtg
     2581 ataccattgc actgtgtaca gcagaatcca tcgataccct ccgagcagca attgaatgtg
     2641 aacagcctca acctgacctc tacaaatttg ttgggcgaat caatatctac agtaatagtc
     2701 ttgaggctgt tgccaggtct ttgggacctg aaaatctctt gctgaaagga gctacgctaa
     2761 aaaataccga gaagatatat ggagttgctg tttacactgg aatggaaacc aaaatggctt
     2821 tgaactacca agggaaatct cagaaacgtt ctgctgttga aaaatctatt aatgctttcc
     2881 tgattgtata tttatttatc ttactgacca aagctgcagt atgcactact ctaaagtatg
     2941 tttggcaaag taccccatac aatgatgaac cttggtataa ccaaaagact cagaaagagc
     3001 gagagacctt gaaggtttta aaaatgttca ccgacttcct atcatttatg gttctattca
     3061 actttatcat tcctgtctcc atgtacgtca cagtagaaat gcagaaattc ttgggctcct
     3121 tcttcatctc atgggataag gacttttatg atgaagaaat taatgaagga gccctggtta
     3181 acacatcaga ccttaatgaa gaacttggtc aggtggatta tgtatttaca gataagactg
     3241 gaacactcac tgaaaacagc atggaattca ttgaatgctg catagatggc cacaaatata
     3301 aaggtgtaac tcaagaggtt gatggattat ctcaaactga tggaacttta acatattttg
     3361 acaaagtaga taagaatcga gaagagctgt ttctacgtgc cttgtgttta tgtcatactg
     3421 tagaaatcaa aacaaacgat gctgttgatg gagctacaga atcagctgaa ttaacctata
     3481 tctcctcttc accagatgaa atagctttgg tgaaaggagc taaaaggtac gggttcacat
     3541 ttttaggaaa tcgaaatgga tatatgagag tagagaacca aagaaaagaa atagaagaat
     3601 atgaacttct tcacacctta aactttgatg ctgtccggcg acgtatgagt gtaattgtga
     3661 agactcaaga aggagacata cttctctttt gtaaaggagc agactcggca gtttttccca
     3721 gagtgcaaaa tcatgaaatt gagttaacta aagtccatgt ggaacgtaat gcaatggatg
     3781 ggtatcggac actctgtgta gccttcaaag aaattgctcc agatgattat gaaagaatta
     3841 acagacagct catagaggca aaaatggcct tacaagacag agaagaaaaa atggaaaaag
     3901 ttttcgatga tattgagaca aacatgaatt taattggagc cactgcagtt gaagacaagc
     3961 tacaagatca agctgcagag accattgaag ctctgcatgc agcaggcctg aaagtctggg
     4021 tgctcactgg ggacaagatg gagacagcta aatccacatg ctatgcctgc cgccttttcc
     4081 agaccaacac tgagctctta gaactaacca caaaaaccat tgaagaaagt gaaaggaaag
     4141 aagatcgatt acatgaatta ttgatagaat atcgcaagaa attgctgcat gagtttccta
     4201 aaagtactag aagctttaaa aaagcatgga cagaacatca ggaatatgga ttaatcatag
     4261 atggctccac attgtcactc atactaaatt ctagtcaaga ctctagttca aacaattaca
     4321 aaagcatttt cctacaaata tgtatgaagt gtactgcagt gctctgctgt cggatggcac
     4381 cattacagaa agcccagatt gtcagaatgg tgaagaattt aaaaggcagc ccaataactc
     4441 tgtcgatagg tgatggtgcc aatgatgtta gtatgatctt ggaatcccat gtgggaatag
     4501 gtattaaagg caaagaaggt cgccaagcag ctaggaatag cgattattct gttccaaagt
     4561 ttaaacactt aaagaaactg ctgttggctc atggacatct atattatgtg agaatagcac
     4621 accttgtaca gtacttcttc tataagaacc tttgtttcat tttgccacag tttttgtacc
     4681 agttcttctg tggattctca caacagccac tgtatgatgc tgcttacctt acaatgtaca
     4741 atatctgctt cacatccttg cccatcctgg cctatagtct actggaacag cacatcaaca
     4801 ttgacactct gacctcagat ccccgattgt atatgaaaat ttctggcaat gccatgctac
     4861 agttgggccc cttcttatat tggacatttc tggctgcctt tgaagggaca gtgttcttct
     4921 ttgggactta ctttcttttt cagactgcat ccctagaaga aaatggaaag gtatacggaa
     4981 actggacttt tggaaccatt gtttttacag tcttagtatt cactgtaacc ctgaagcttg
     5041 ccttggatac ccgattctgg acgtggataa atcactttgt gatttggggt tctttagcct
     5101 tctatgtatt tttctcattc ttctggggag gaattatttg gccttttctc aagcaacaga
     5161 gaatgtattt tgtatttgcc caaatgctgt cttctgtatc cacatggttg gctataattc
     5221 ttctaatatt tatcagcctg ttccctgaga ttcttctgat agtattaaag aatgtaagaa
     5281 gaagaagtgc caggagaaat ctgagctgta gaagggcatc tgactcatta tccgccagac
     5341 cttcagtcag acctcttctt ttacgaacat tctcagacga atctaatgta ctctcgagat
     5401 atctagaccc agctttcttg tacaaagtgg ttgatctaga gggcccgcgg ttcgaactcg
     5461 agtatccata cgatgtacca gactacgcag agatccacta gttcaattcc agctgagcgc
     5521 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
     5581 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaactcga gcggccgcca
     5641 gcacagtggt cgactgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5701 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5761 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5821 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5881 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5941 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttca
     6001 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     6061 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     6121 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     6181 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     6241 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     6301 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     6361 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6421 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6481 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6541 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6601 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6661 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6721 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6781 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6841 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6901 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6961 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     7021 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     7081 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7141 tgagcgggac tctggggttc gcatcgtcga cgataaaata aaagatttta tttagtctcc
     7201 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     7261 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
     7321 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     7381 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     7441 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     7501 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     7561 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     7621 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
     7681 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     7741 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     7801 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa
     7861 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7921 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7981 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8041 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8521 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat
     8821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt c
//
