LOCUS       sequence-413013-       16301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G-Deleted Rabies vector expressing H2B-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16301)
  AUTHORS   Patino M, Lagos WN, Patne NS, Tasic B, Zeng H, Callaway EM
  TITLE     Single-cell transcriptomic classification of rabies-infected 
            cortical neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 May 31;119(22):e2203677119. doi: 
            10.1073/pnas.2203677119. Epub 2022 May 24.
  PUBMED    35609197
REFERENCE   2  (bases 1 to 16301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-05-31"; volume: "119(22):e2203677119. 
            doi"; pages: " 10.1073/pnas.2203677119. Epub 2022 May 24"
COMMENT     Sequence Label: pSAD-dG-F3-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..16301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4279..4656
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4705..4714
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4711..5421
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4856..4874)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5124..5143
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7200..7211
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12309..12326)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12315..12539
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12585..13013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12672..12691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12882..12903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13022..13042)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13027..13356
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13207..13342
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13269..13288
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13404..13451
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13470..13844
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13974..14095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14011..14030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14065..14084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14144..14160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14144..14160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14157..14179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14168..14184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14192..14198,14199..14216,14217..14222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14237..14258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14375..14392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14546..15134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14626..14645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15305..16096,16097..16165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15928..15947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16166..16270)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 ttaagcgtct gatgagtccg tgaggacgaa acccggagtc ccgggtcacg cttaacaacc
      961 agatcaaaga aaaaacagac attgtcaatt gcaaagcaaa aatgtaacac ccctacaatg
     1021 gatgccgaca agattgtatt caaagtcaat aatcaggtgg tctctttgaa gcctgagatt
     1081 atcgtggatc aatatgagta caagtaccct gccatcaaag atttgaaaaa gccctgtata
     1141 accctaggaa aggctcccga tttaaataaa gcatacaagt cagttttgtc aggcatgagc
     1201 gccgccaaac ttaatcctga cgatgtatgt tcctatttgg cagcggcaat gcagtttttt
     1261 gaggggacat gtccggaaga ctggaccagc tatggaattg tgattgcacg aaaaggagat
     1321 aagatcaccc caggttctct ggtggagata aaacgtactg atgtagaagg gaattgggct
     1381 ctgacaggag gcatggaact gacaagagac cccactgtcc ctgagcatgc gtccttagtc
     1441 ggtcttctct tgagtctgta taggttgagc aaaatatccg ggcaaaacac tggtaactat
     1501 aagacaaaca ttgcagacag gatagagcag atttttgaga cagccccttt tgttaaaatc
     1561 gtggaacacc atactctaat gacaactcac aaaatgtgtg ctaattggag tactatacca
     1621 aacttcagat ttttggccgg aacctatgac atgtttttct cccggattga gcatctatat
     1681 tcagcaatca gagtgggcac agttgtcact gcttatgaag actgttcagg actggtatca
     1741 tttactgggt tcataaaaca aatcaatctc accgctagag aggcaatact atatttcttc
     1801 cacaagaact ttgaggaaga gataagaaga atgtttgagc cagggcagga gacagctgtt
     1861 cctcactctt atttcatcca cttccgttca ctaggcttga gtgggaaatc tccttattca
     1921 tcaaatgctg ttggtcacgt gttcaatctc attcactttg taggatgcta tatgggtcaa
     1981 gtcagatccc taaatgcaac ggttattgct gcatgtgctc ctcatgaaat gtctgttcta
     2041 gggggctatc tgggagagga attcttcggg aaagggacat ttgaaagaag attcttcaga
     2101 gatgagaaag aacttcaaga atacgaggcg gctgaactga caaagactga cgtagcactg
     2161 gcagatgatg gaactgtcaa ctctgacgac gaggactact tttcaggtga aaccagaagt
     2221 ccggaggctg tttatactcg aatcatgatg aatggaggtc gactaaagag atctcacata
     2281 cggagatatg tctcagtcag ttccaatcat caagcccgtc caaactcatt cgccgagttt
     2341 ctaaacaaga catattcgag tgactcataa gaagttgaat aacaaaatgc cggaaatcta
     2401 cggattgtgt atatccatca tgaaaaaaac taacacccct cctttcgaac catcccaaac
     2461 atgagcaaga tctttgtcaa tcctagtgct attagagccg gtctggccga tcttgagatg
     2521 gctgaagaaa ctgttgatct gatcaataga aatatcgaag acaatcaggc tcatctccaa
     2581 ggggaaccca tagaggtgga caatctccct gaggatatgg ggcgacttca cctggatgat
     2641 ggaaaatcgc ccaaccatgg tgagatagcc aaggtgggag aaggcaagta tcgagaggac
     2701 tttcagatgg atgaaggaga ggatcctagc ttcctgttcc agtcatacct ggaaaatgtt
     2761 ggagtccaaa tagtcagaca aatgaggtca ggagagagat ttctcaagat atggtcacag
     2821 accgtagaag agattatatc ctatgtcgcg gtcaactttc ccaaccctcc aggaaagtct
     2881 tcagaggata aatcaaccca gactactggc cgagagctca agaaggagac aacacccact
     2941 ccttctcaga gagaaagcca atcatcgaaa gccaggatgg cggctcaaat tgcttctggc
     3001 cctccagccc ttgaatggtc ggctaccaat gaagaggatg atctatcagt ggaggctgag
     3061 atcgctcacc agattgcaga aagtttctcc aaaaaatata agtttccctc tcgatcctca
     3121 gggatactct tgtataattt tgagcaattg aaaatgaacc ttgatgatat agttaaagag
     3181 gcaaaaaatg taccaggtgt gacccgttta gcccatgacg ggtccaaact ccccctaaga
     3241 tgtgtactgg gatgggtcgc tttggccaac tctaagaaat tccagttgtt agtcgaatcc
     3301 gacaagctga gtaaaatcat gcaagatgac ttgaatcgct atacatcttg ctaaccgaac
     3361 ctctcccctc agtccctcta gacaataaaa tccgagatgt cccaaagtca acatgaaaaa
     3421 aacaggcaac accactgata aatcgatgaa cctcctacgt aagatagtga aaaaccgcag
     3481 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3541 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     3601 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     3661 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     3721 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     3781 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     3841 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     3901 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     3961 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4021 agaggacaaa gattcctctc tgcttctaga ataacgtacg tcagattata tcccgcaaat
     4081 ttatcacttg tttacctctg gaggagagaa catatgggct caactccaac ccttgggagc
     4141 aatataacaa aaaacatgtt atggtgccat taaaccgctg catttcatca aagtcaagtt
     4201 gattaccttt acattttgat cctcttggat gtgaaaaaaa ctattaacat ccctcaaagg
     4261 acctgcaggg ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
     4321 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
     4381 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
     4441 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
     4501 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
     4561 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
     4621 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc acggtcacgg tactggttct
     4681 actggttctg gttcttctcc ggtcgccacc atggtgagca agggcgagga ggataacatg
     4741 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     4801 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     4861 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     4921 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     4981 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     5041 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     5101 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     5161 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     5221 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     5281 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     5341 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     5401 atggacgagc tgtacaagta agctagcatg aaaaaaacta acacccctcc ttaattaata
     5461 cggcgcgcct acccgcggta gcttttcagt cgagaaaaaa acattagatc agaagaacaa
     5521 ctggcaacac ttctcaacct gagacttact tcaagatgct cgatcctgga gaggtctatg
     5581 atgaccctat tgacccaatc gagttagagg ctgaacccag aggaaccccc attgtcccca
     5641 acatcttgag gaactctgac tacaatctca actctccttt gatagaagat cctgctagac
     5701 taatgttaga atggttaaaa acagggaata gaccttatcg gatgactcta acagacaatt
     5761 gctccaggtc tttcagagtt ttgaaagatt atttcaagaa ggtagatttg ggttctctca
     5821 aggtgggcgg aatggctgca cagtcaatga tttctctctg gttatatggt gcccactctg
     5881 aatccaacag gagccggaga tgtataacag acttggccca tttctattcc aagtcgtccc
     5941 ccatagagaa gctgttgaat ctcacgctag gaaatagagg gctgagaatc cccccagagg
     6001 gagtgttaag ttgccttgag agggttgatt atgataatgc atttggaagg tatcttgcca
     6061 acacgtattc ctcttacttg ttcttccatg taatcacctt atacatgaac gccctagact
     6121 gggatgaaga aaagaccatc ctagcattat ggaaagattt aacctcagtg gacatcggga
     6181 aggacttggt aaagttcaaa gaccaaatat ggggactgct gatcgtgaca aaggactttg
     6241 tttactccca aagttccaat tgtctttttg acagaaacta cacacttatg ctaaaagatc
     6301 ttttcttgtc tcgcttcaac tccttaatgg tcttgctctc tcccccagag ccccgatact
     6361 cagatgactt gatatctcaa ctatgccagc tgtacattgc tggggatcaa gtcttgtcta
     6421 tgtgtggaaa ctccggctat gaagtcatca aaatattgga gccatatgtc gtgaatagtt
     6481 tagtccagag agcagaaaag tttaggcctc tcattcattc cttgggagac tttcctgtat
     6541 ttataaaaga caaggtaagt caacttgaag agacgttcgg tccctgtgca agaaggttct
     6601 ttagggctct ggatcaattc gacaacatac atgacttggt ttttgtgttt ggctgttaca
     6661 ggcattgggg gcacccatat atagattatc gaaagggtct gtcaaaacta tatgatcagg
     6721 ttcaccttaa aaaaatgata gataagtcct accaggagtg cttagcaagc gacctagcca
     6781 ggaggatcct tagatggggt tttgataagt actccaagtg gtatctggat tcaagattcc
     6841 tagcccgaga ccaccccttg actccttata tcaaaaccca aacatggcca cccaaacata
     6901 ttgtagactt ggtgggggat acatggcaca agctcccgat cacgcagatc tttgagattc
     6961 ctgaatcaat ggatccgtca gaaatattgg atgacaaatc acattctttc accagaacga
     7021 gactagcttc ttggctgtca gaaaaccgag gggggcctgt tcctagcgaa aaagttatta
     7081 tcacggccct gtctaagccg cctgtcaatc cccgagagtt tctgaggtct atagacctcg
     7141 gaggattgcc agatgaagac ttgataattg gcctcaagcc aaaggaacgg gaattgaaga
     7201 ttgaaggtcg attctttgct ctaatgtcat ggaatctaag attgtatttt gtcatcactg
     7261 aaaaactctt ggccaactac atcttgccac tttttgacgc gctgactatg acagacaacc
     7321 tgaacaaggt gtttaaaaag ctgatcgaca gggtcaccgg gcaagggctt ttggactatt
     7381 caagggtcac atatgcattt cacctggact atgaaaagtg gaacaaccat caaagattag
     7441 agtcaacaga ggatgtattt tctgtcctag atcaagtgtt tggattgaag agagtgtttt
     7501 ctagaacaca cgagtttttt caaaaggcct ggatctatta ttcagacaga tcagacctca
     7561 tcgggttacg ggaggatcaa atatactgct tagatgcgtc caacggccca acctgttgga
     7621 atggccagga tggcgggcta gaaggcttac ggcagaaggg ctggagtcta gtcagcttat
     7681 tgatgataga tagagaatct caaatcagga acacaagaac caaaatacta gctcaaggag
     7741 acaaccaggt tttatgtccg acatacatgt tgtcgccagg gctatctcaa gaggggctcc
     7801 tctatgaatt ggagagaata tcaaggaatg cactttcgat atacagagcc gtcgaggaag
     7861 gggcatctaa gctagggctg atcatcaaga aagaagagac catgtgtagt tatgacttcc
     7921 tcatctatgg aaaaacccct ttgtttagag gtaacatatt ggtgcctgag tccaaaagat
     7981 gggccagagt ctcttgcgtc tctaatgacc aaatagtcaa cctcgccaat ataatgtcga
     8041 cagtgtccac caatgcgcta acagtggcac aacactctca atctttgatc aaaccgatga
     8101 gggattttct gctcatgtca gtacaggcag tctttcacta cctgctattt agcccaatct
     8161 taaagggaag agtttacaag attctgagcg ctgaagggga gagctttctc ctagccatgt
     8221 caaggataat ctatctagat ccttctttgg gagggatatc tggaatgtcc ctcggaagat
     8281 tccatatacg acagttctca gaccctgtct ctgaagggtt atccttctgg agagagatct
     8341 ggttaagctc ccaagagtcc tggattcacg cgttgtgtca agaggctgga aacccagatc
     8401 ttggagagag aacactcgag agcttcactc gccttctaga agatccgacc accttaaata
     8461 tcagaggagg ggccagtcct accattctac tcaaggatgc aatcagaaag gctttatatg
     8521 acgaggtgga caaggtggaa aattcagagt ttcgagaggc aatcctgttg tccaagaccc
     8581 atagagataa ttttatactc ttcttaatat ctgttgagcc tctgtttcct cgatttctca
     8641 gtgagctatt cagttcgtct tttttgggaa tccccgagtc aatcattgga ttgatacaaa
     8701 actcccgaac gataagaagg cagtttagaa agagtctctc aaaaacttta gaagaatcct
     8761 tctacaactc agagatccac gggattagtc ggatgaccca gacacctcag agggttgggg
     8821 gggtgtggcc ttgctcttca gagagggcag atctacttag ggagatctct tggggaagaa
     8881 aagtggtagg cacgacagtt cctcaccctt ctgagatgtt gggattactt cccaagtcct
     8941 ctatttcttg cacttgtgga gcaacaggag gaggcaatcc tagagtttct gtatcagtac
     9001 tcccgtcctt tgatcagtca tttttttcac gaggccccct aaagggatac ttgggctcgt
     9061 ccacctctat gtcgacccag ctattccatg catgggaaaa agtcactaat gttcatgtgg
     9121 tgaagagagc tctatcgtta aaagaatcta taaactggtt cattactaga gattccaact
     9181 tggctcaagc tctaattagg aacattatgt ctctgacagg ccctgatttc cctctagagg
     9241 aggcccctgt cttcaaaagg acggggtcag ccttgcatag gttcaagtct gccagataca
     9301 gcgaaggagg gtattcttct gtctgcccga acctcctctc tcatatttct gttagtacag
     9361 acaccatgtc tgatttgacc caagacggga agaactacga tttcatgttc cagccattga
     9421 tgctttatgc acagacatgg acatcagagc tggtacagag agacacaagg ctaagagact
     9481 ctacgtttca ttggcacctc cgatgcaaca ggtgtgtgag acccattgac gacgtgaccc
     9541 tggagacctc tcagatcttc gagtttccgg atgtgtcgaa aagaatatcc agaatggttt
     9601 ctggggctgt gcctcacttc cagaggcttc ccgatatccg tctgagacca ggagattttg
     9661 aatctctaag cggtagagaa aagtctcacc atatcggatc agctcagggg ctcttatact
     9721 caatcttagt ggcaattcac gactcaggat acaatgatgg aaccatcttc cctgtcaaca
     9781 tatacggcaa ggtttcccct agagactatt tgagagggct cgcaagggga gtattgatag
     9841 gatcctcgat ttgcttcttg acaagaatga caaatatcaa tattaataga cctcttgaat
     9901 tggtctcagg ggtaatctca tatattctcc tgaggctaga taaccatccc tccttgtaca
     9961 taatgctcag agaaccgtct cttagaggag agatattttc tatccctcag aaaatccccg
    10021 ccgcttatcc aaccactatg aaagaaggca acagatcaat cttgtgttat ctccaacatg
    10081 tgctacgcta tgagcgagag ataatcacgg cgtctccaga gaatgactgg ctatggatct
    10141 tttcagactt tagaagtgcc aaaatgacgt acctatccct cattacttac cagtctcatc
    10201 ttctactcca gagggttgag agaaacctat ctaagagtat gagagataac ctgcgacaat
    10261 tgagttcttt gatgaggcag gtgctgggcg ggcacggaga agatacctta gagtcagacg
    10321 acaacattca acgactgcta aaagactctt tacgaaggac aagatgggtg gatcaagagg
    10381 tgcgccatgc agctagaacc atgactggag attacagccc caacaagaag gtgtcccgta
    10441 aggtaggatg ttcagaatgg gtctgctctg ctcaacaggt tgcagtctct acctcagcaa
    10501 acccggcccc tgtctcggag cttgacataa gggccctctc taagaggttc cagaaccctt
    10561 tgatctcggg cttgagagtg gttcagtggg caaccggtgc tcattataag cttaagccta
    10621 ttctagatga tctcaatgtt ttcccatctc tctgccttgt agttggggac gggtcagggg
    10681 ggatatcaag ggcagtcctc aacatgtttc cagatgccaa gcttgtgttc aacagtcttt
    10741 tagaggtgaa tgacctgatg gcttccggaa cacatccact gcctccttca gcaatcatga
    10801 ggggaggaaa tgatatcgtc tccagagtga tagatcttga ctcaatctgg gaaaaaccgt
    10861 ccgacttgag aaacttggca acctggaaat acttccagtc agtccaaaag caggtcaaca
    10921 tgtcctatga cctcattatt tgcgatgcag aagttactga cattgcatct atcaaccgga
    10981 tcaccctgtt aatgtccgat tttgcattgt ctatagatgg accactctat ttggtcttca
    11041 aaacttatgg gactatgcta gtaaatccaa actacaaggc tattcaacac ctgtcaagag
    11101 cgttcccctc ggtcacaggg tttatcaccc aagtaacttc gtctttttca tctgagctct
    11161 acctccgatt ctccaaacga gggaagtttt tcagagatgc tgagtacttg acctcttcca
    11221 cccttcgaga aatgagcctt gtgttattca attgtagcag ccccaagagt gagatgcaga
    11281 gagctcgttc cttgaactat caggatcttg tgagaggatt tcctgaagaa atcatatcaa
    11341 atccttacaa tgagatgatc ataactctga ttgacagtga tgtagaatct tttctagtcc
    11401 acaagatggt tgatgatctt gagttacaga ggggaactct gtctaaagtg gctatcatta
    11461 tagccatcat gatagttttc tccaacagag tcttcaacgt ttccaaaccc ctaactgacc
    11521 cctcgttcta tccaccgtct gatcccaaaa tcctgaggca cttcaacata tgttgcagta
    11581 ctatgatgta tctatctact gctttaggtg acgtccctag cttcgcaaga cttcacgacc
    11641 tgtataacag acctataact tattacttca gaaagcaagt cattcgaggg aacgtttatc
    11701 tatcttggag ttggtccaac gacacctcag tgttcaaaag ggtagcctgt aattctagcc
    11761 tgagtctgtc atctcactgg atcaggttga tttacaagat agtgaagact accagactcg
    11821 ttggcagcat caaggatcta tccagagaag tggaaagaca ccttcatagg tacaacaggt
    11881 ggatcaccct agaggatatc agatctagat catccctact agactacagt tgcctgtgaa
    11941 ccggatactc ctggaagcct gcccatgcta agactcttgt gtgatgtatc ttgaaaaaaa
    12001 caagatccta aatctgaacc tttggttgtt tgattgtttt tctcattttt gttgtttatt
    12061 tgttaagcgt gggtcggcat ggcatctcca cctcctcgcg gtccgacctg ggcatccgaa
    12121 ggaggacgca cgtccactcg gatggctaag ggagaagggc gaattccagc acactggcgg
    12181 ccgttactag tggatccgag ctcggtaccg agctcggatc cactagtcca gtgtggtgga
    12241 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg
    12301 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    12361 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    12421 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    12481 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
    12541 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
    12601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    12661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    12721 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    12781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    12841 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    12901 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    12961 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
    13021 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    13081 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    13141 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    13201 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    13261 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
    13321 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
    13381 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
    13441 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
    13501 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
    13561 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
    13621 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
    13681 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
    13741 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
    13801 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
    13861 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
    13921 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
    13981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    14041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    14101 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
    14161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    14221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    14281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    14341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    14401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    14461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    14521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    14581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    14641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    14701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    14761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    14821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    14881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    14941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    15001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    15061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    15121 aaaaagcatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    15181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    15241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    15301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    15361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    15421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    15481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    15541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    15601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    15661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    15721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    15781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    15841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    15901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    15961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    16021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    16081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    16141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    16201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    16261 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
