LOCUS       sequence-413014-        7436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat beta1b calcium channel auxillary subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7436)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: cacnb1b pCAGGS
FEATURES             Location/Qualifiers
     source          1..7436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4457..4476)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4522..4577
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4576..4595)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4938..4954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4938..4954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4951..4973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4962..4978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4986..4992,4993..5010,5011..5016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5031..5052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5111..5306
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5157..5292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5312..5446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5349..5368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5403..5422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5514..5531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5685..6273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5765..5784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6444..7235,7236..7304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7067..7086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7305..7409)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggsgcg ccaggggggg gggggggggg ggaggggggg
      541 ggsggggsga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccggggr gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cggcacgaga
     1741 gcgtgcagct gggcgactgc acccggtgct ggctgcgcga cgccgcgctg ctctcggctc
     1801 ggacggcctc tcccatgcgc tgagggcccc cggctgtgcc gggagggcgg ccggaccgga
     1861 ggatcctctc catggtccag aagagcggca tgtcccgggg cccttaccca ccttcccaag
     1921 agatccctat ggaggtcttc gaccccagcc cacagggcaa atacagcaag aggaaagggc
     1981 ggttcaaaag gtcagatggg agcacctcct cagataccac atccaacagc tttgtccgtc
     2041 agggctcagc agagtcctac acgagccggc cgtcagactc cgacgtgtcc ctggaggagg
     2101 accgggaagc cttaaggaag gaggcagagc gccaggcctt agcccagctc gagaaagcca
     2161 agaccaaacc agtggccttt gctgttcgga caaatgttgg ctacaatccg tctccaggag
     2221 atgaggtgcc agtgcaggga gtggccatca cctttgagcc caaggacttc ctgcacatca
     2281 aggagaaata caataatgac tggtggatcg ggaggctggt gaaggaaggc tgtgaggttg
     2341 gtttcatccc cagccctgtc aaactggaca gccttcgcct gctgcaggaa cagacgctgc
     2401 gccaaaaccg cctcagctcc agcaagtcag gtgacaactc cagttccagt ctgggagatg
     2461 tggtgactgg cacccgccgc cccacacccc ccgccagtgc caaacagaag cagaagtcga
     2521 cagagcacgt gcccccctat gacgtggtgc cttccatgag gcccatcatc ctggtgggac
     2581 catcgctcaa gggctatgag gtaactgaca tgatgcagaa ggcgttgttt gacttcttga
     2641 agcatcggtt tgatggcagg atttccatca cccgggtaac ggctgacatt tccctggcca
     2701 agcgctcggt cctcaacaac cccagcaaac acattatcat cgagcgctcc aacactcgct
     2761 ccagcctggc tgaggtacag agtgaaatcg agcggatctt cgagctggcc cggaccttgc
     2821 agctggttgc cctggacgcc gacaccatca accacccagc ccagctctct aaaacctcgc
     2881 tggcccccat cattgtttac atcaagatca catcccccaa ggtactgcag aggctcatca
     2941 aatcccgagg gaagtctcaa tccaaacacc tcaatgtcca aatagcagcc tcggagaagc
     3001 tggcacagtg tccccccgaa atgtttgaca taatcctgga cgagaaccaa ttggaagatg
     3061 cctgcgagca cctggcggag tacttggaag cctactggaa ggccacacac ccgcctagca
     3121 gcacgccacc caatccgctg ctgaaccgca ccatggctac cgccgctctg gctgccagcc
     3181 ctgcccccgt ctccaacctc cagggaccct accttgcttc cggggaccag ccgctggacc
     3241 gggccactgg ggagcatgcc agtgtgcacg agtaccccgg ggagctgggc cagcccccag
     3301 gcctttaccc cagcaaccac ccacctggcc gggcaggcac cctgcgggcg ctatcccgcc
     3361 aagacacctt tgatgctgac acccccggca gccgaaactc tgcctacacg gagccaggag
     3421 actcgtgtgt ggacatggag acagacccct cagagggccc agggcctgga gaccctgcag
     3481 ggggaggtac accaccagct cggcagggct cctgggaaga ggaggaagat tatgaggagg
     3541 agatgaccga caacaggaac cggggccgga ataaggcccg ctactgtgcg gagggtggtg
     3601 ggccggttct ggggcgcaat aagaatgagc tggagggctg gggacaaggc gtctacatcc
     3661 gctgagaggc aggggcagtt ctgggagaga agaggctctg aactcgggga ggggagggca
     3721 gaggggcgca ccacccaaga tgtgtcttgc ctccaggggg cgctttgtcc ctcattttca
     3781 gatgcctttg ctcaaagctt ggggtttctt tggtggttat cattcccaac tcctgggagg
     3841 tccttaatcc ccagctgtag gtttttccct aaactgctgt gggtggatgg gggaatacct
     3901 actttctgaa gtgccccctt tccccatctt tggggctttc tccctcccag agaaaaggtg
     3961 cacttcctta actttttact cggggctgta agcggaggtc ctaattgggg tggggtggag
     4021 gggtttcttc taccaacgca ggggtacttg ggaaaggttg ggtgcttttc ctgaagagaa
     4081 cccgctgaat cttccacaaa ccccagaccc gggatccccc accatatcca ttccataacc
     4141 tagtttccta gcagcattct ctggtgggtg aaagccacag cttggaaacc catgaagagc
     4201 ttgctttggg gagcagagct gggtgcaaac tgacgggcat cagtgtgtgc tctctgccct
     4261 ccatggtcat ggcaagaaag cagggcctag aaacttgagg agtgagcaac tcaagatgct
     4321 gcctcgtgcc gaattcgcat gcggccgcta gctcgaggta ccgatatcga gctcaattca
     4381 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     4441 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     4501 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     4561 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     4621 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     4681 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     4741 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     4801 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     4861 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     4921 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4981 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5041 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5101 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5161 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5221 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5281 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     5341 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5401 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     5461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgg
//
