LOCUS       sequence-413020-        9669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8A1 mutant E191Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pCAG/ATP8A1(E191Q)-HA
FEATURES             Location/Qualifiers
     source          1..9669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..22)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    68..123
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(122..141)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(484..500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(484..500)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(497..519)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    508..524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(532..538,539..556,557..562))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    577..598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        657..852
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      703..838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    858..992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(895..914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     949..968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1060..1077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1231..1819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1311..1330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1990..2781,2782..2850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2613..2632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2851..2955)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3267..4070
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3330..3349)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3940..3959
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4077..4125
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4135..4514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4855..5871
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5795..5817
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5879..5898
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    5967..5991
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9490..9514)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9558..9584
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
       61 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
      121 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
      181 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
      241 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
      301 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
      361 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
      421 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
      481 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      541 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      601 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
      661 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
      721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
      781 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
      841 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      901 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      961 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     1021 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1081 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1141 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1201 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1261 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1321 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1381 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1441 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1501 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1561 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1621 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1681 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1741 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1801 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1861 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1921 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1981 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2041 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2101 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2161 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2221 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2281 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2341 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2401 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2461 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2521 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2581 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2641 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2701 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2761 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2821 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2881 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2941 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgagca gtgtggtttt
     3001 caagaggaag caaaaagcct ctccacccag gcctggaatg tttccaccca atgtcgagca
     3061 gtgtggtttt gcaagaggaa gcaaaaagcc tctccaccca ggcctggaat gtttccaccc
     3121 aatgtcgagc aaaccccgcc cagcgtcttg tcattggcga attaattcga acacgcagat
     3181 gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
     3241 gaacaccgag cgaccctgca gccaatatgg gatcggccat tgaacaagat ggattgcacg
     3301 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3361 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3421 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3481 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3541 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3601 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3661 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3721 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3781 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg
     3841 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3901 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3961 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4021 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcggc
     4081 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcggatc cgtcgacatt
     4141 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4201 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     4261 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4321 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     4381 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4441 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4501 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     4561 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     4621 cggggggggg ggggggggcg cgccagtggg gggtgggggg ggcggggggg gggggggggg
     4681 gggggggggg ggggggggcg cggggggggc ggggcggggg ggggcggggg gcggggcggg
     4741 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     4801 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     4861 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     4921 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     4981 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     5041 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     5101 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     5161 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     5221 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     5281 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     5341 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     5401 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     5461 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     5521 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     5581 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     5641 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     5701 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     5761 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     5821 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     5881 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aatttgctag cgagctcgcg
     5941 gccgcagatc tctagttaag ctatcaacaa gtttgtacaa aaaagcaggc tctttaaagg
     6001 aaccaattca gtcgactgga tccaccatgc ccaccatgcg gaggaccgtg tcggagatcc
     6061 gctcgcgcgc cgaaggttat gagaagacag atgatgtttc agagaagacc tcactggctg
     6121 accaggagga agtaaggact attttcatca accagcccca gctgacaaaa ttctgcaata
     6181 accatgtcag cactgcaaaa tacaacataa tcacattcct tccaagattt ctctactctc
     6241 agttcagaag agctgctaat tcattttttc tctttattgc actgctgcag caaatacctg
     6301 atgtgtcacc aacaggtcgt tatacaacac tggttcctct cttatttatt ttagctgtgg
     6361 cagctatcaa agagataata gaagatatta aacgacataa agctgataat gcagtgaaca
     6421 agaaacaaac gcaagttttg agaaatggtg cttgggaaat tgtccactgg gaaaaggtaa
     6481 atgttggaga tatagttata ataaaaggca aagagtatat acctgctgac actgtacttc
     6541 tctcatcaag tgagccccaa gccatgtgct acattgaaac atccaactta gatggtcaaa
     6601 caaacttgaa aattagacag ggcttaccag caacatcaga tatcaaagac gttgacagtt
     6661 tgatgaggat ttctggcaga attgagtgtg aaagtccaaa cagacatctc tacgattttg
     6721 ttggaaacat aaggcttgat ggacatggca ccgttccact gggagcagat cagattcttc
     6781 ttcgaggagc tcagttgaga aatacacagt gggttcatgg aatagttgtc tacactggac
     6841 atgacaccaa gctgatgcag aattcaacaa gtccaccact taagctctca aatgtggaac
     6901 ggattacaaa tgtacaaatt ttgattttat tttgtatctt aattgccatg tctcttgtct
     6961 gttctgtggg ctcagccatt tggaatcgaa ggcattctgg aaaagactgg tatctcaatc
     7021 taaactatgg tggcgctagt aattttggac tgaatttctt gaccttcatc atccttttca
     7081 acaatctcat tcctatcagc ttattggtta cattagaagt tgtgaaattt acccaggcat
     7141 acttcataaa ttgggatctt gacatgcact atgaacccac agacactgct gctatggctc
     7201 gaacatctaa tctgaatgag gaacttggcc aggttaaata catattttct gacaaaactg
     7261 gtactctgac atgcaatgta atgcagttta agaagtgcac catagcggga gttgcttatg
     7321 ggcagaactc acagtttgga gatgaaaaaa catttagtga ttcatcattg ctggaaaatc
     7381 tccaaaataa tcatccaact gcacctataa tatgtgaatt tcttacaatg atggcagtct
     7441 gtcacacagc agtgccagag cgagaaggtg acaagattat ttatcaagca gcatctccag
     7501 atgagggagc attggtcaga gcagccaagc aattgaattt tgttttcact ggaagaacac
     7561 ccgactcggt gattatagat tcactggggc aggaagaaag atatgaattg ctcaatgtct
     7621 tggagtttac cagtgctagg aaaagaatgt cagtgattgt tcgcactcca tctggaaagt
     7681 tacgactcta ctgcaaagga gctgacactg taatttatga tcgactggca gagacgtcaa
     7741 aatacaaaga aattacccta aaacatttag agcagtttgc tacagaaggg ttaagaactt
     7801 tatgttttgc tgtggctgag atttcagaga gcgactttca ggagtggcga gcagtctatc
     7861 agcgagcatc tacatctgtg cagaacaggc tactcaaact cgaagagagt tatgagttga
     7921 ttgaaaagaa tcttcagcta cttggagcaa cagccattga ggataaatta caagatcaag
     7981 tgcctgaaac catagaaacg ctaatgaaag cagacatcaa aatctggatc cttacagggg
     8041 acaagcaaga aactgccatt aacatcggac actcctgcaa actgttgaag aagaacatgg
     8101 gaatgattgt tataaatgaa ggctctcttg atggaacaag ggaaactctc agtcgtcact
     8161 gtactaccct tggtgatgct ctccggaaag agaatgattt tgctcttata attgatggga
     8221 aaaccctcaa atatgcctta acctttggag tacgacagta tttcctggac ttagctttgt
     8281 catgcaaagc tgtcatttgc tgtcgggttt ctcctcttca aaaatctgaa gttgttgaga
     8341 tggttaagaa acaagtcaaa gtcgtaacgc ttgcaatcgg tgatggagca aatgatgtca
     8401 gcatgataca gacagcgcac gttggtgttg gtatcagtgg caatgaaggc ctgcaggcag
     8461 ctaattcctc tgactactcc atagctcagt tcaaatattt gaagaattta ctgatgattc
     8521 atggtgcctg gaactataac agagtctcca agtgcatctt atactgcttc tacaagaata
     8581 tagtgctcta tattatcgag atctggtttg cctttgttaa tggcttttct ggacagatcc
     8641 tctttgaaag atggtgtata ggtctctata acgtgatgtt tacagcaatg cctcctttaa
     8701 ctcttggaat atttgagaga tcatgcagaa aagagaacat gttgaagtac cctgaattat
     8761 acaaaacatc tcagaatgcc ctggacttca acaccaaggt tttctgggtt cattgtttaa
     8821 atggcctctt ccactcagtt attctgtttt ggtttccact aaaagccctt cagtatggta
     8881 ctgcatttgg aaatgggaaa acctcggatt atctgctact gggaaacttt gtgtacactt
     8941 ttgtggtgat aactgtgtgt ttgaaagctg gattggagac atcatattgg acatggttca
     9001 gccacatagc gatatggggg agcatcgcac tctgggtggt gttttttgga atctactcat
     9061 ctctgtggcc tgccattccg atggcccctg atatgtcagg agaggcagcc atgttgttca
     9121 gttctggagt cttttggatg ggcttgttat tcatccctgt ggcatctctg ctccttgatg
     9181 tggtgtacaa ggttatcaag aggactgctt ttaaaacatt ggtcgatgaa gttcaggagc
     9241 tggaggcaaa atctcaagac ccaggagcag ttgtacttgg aaaaagcctg accgagaggg
     9301 cgcaactgct caagaacgtc tttaagaaga accacgtgaa cttgtaccgc tctgaatcct
     9361 tgcaacaaaa tctgctccat gggtatgcgt tctctcaaga tgaaaatgga atcgtttcac
     9421 agtctgaagt gataagagca tatgatacca cgaaacagag gcccgacgaa tgggcctcga
     9481 gatatctaga cccagctttc ttgtacaaag tggttgatct agagggcccg cggttcgaac
     9541 tcgaggtacc gaattcgtac ccatacgacg tcccagacta cgcttaattc actcctcagg
     9601 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     9661 actgagatc
//
