LOCUS       sequence-413042-        9911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B1 mutant E234Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pCAG/ATP8B1(E234Q)-HA
FEATURES             Location/Qualifiers
     source          1..9911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     intron          1930..2946
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2870..2892
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2954..2973
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3042..3066
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5200..5211
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(5565..5576)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(6865..6889)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6933..6959
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7047..7066)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7112..7167
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7166..7185)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7528..7544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7528..7544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7541..7563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7552..7568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7576..7582,7583..7600,7601..7606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7621..7642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7701..7896
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7747..7882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7902..8036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7939..7958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7993..8012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8104..8121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8275..8863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8355..8374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9034..9825,9826..9894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9657..9676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9895..9911,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact atcctgtatg tgctaccacc
     1621 ccctaagtat tctccgccct tctcaccccg cgcccgctcc ccccccccta tttctctcat
     1681 ccctccaccc cccccccccc ccccacccca gtgtgtggtt gtttgtgtgt tatatgggct
     1741 gggggggggg gggggggggg gggggggggg gggcgggggg gggggggggg ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattt
     3001 gctagcgagc tcgcggccgc agatctctag ttaagctatc aacaagtttg tacaaaaaag
     3061 caggctcttt aaaggaacca attcagtcga ctggatccat gagtacagaa agagactcag
     3121 aaacgacgtt tgacgaggat tctcagccta atgacgaagt ggttccctac agtgatgatg
     3181 aaacagaaga tgaacttgat gaccaggggt ctgctgttga accagaacaa aaccgagtca
     3241 acagggaagc agaggagaac cgggagccat tcagaaaaga atgtacatgg caagtcaaag
     3301 caaacgatcg caagtaccac gaacaacctc actttatgaa cacaaaattc ttgtgtatta
     3361 aggagagtaa atatgcgaat aatgcaatta aaacatacaa gtacaacgca tttaccttta
     3421 taccaatgaa tctgtttgag cagtttaaga gagcagccaa tttatatttc ctggctcttc
     3481 ttatcttaca ggcagttcct caaatctcta ccctggcttg gtacaccaca ctagtgcccc
     3541 tgcttgtggt gctgggcgtc actgcaatca aagacctggt ggacgatgtg gctcgccata
     3601 aaatggataa ggaaatcaac aataggacgt gtgaagtcat taaggatggc aggttcaaag
     3661 ttgctaagtg gaaagaaatt caagttggag acgtcattcg tctgaaaaaa aatgactttg
     3721 ttccagctga cattctcctg ctgtctagct ctgagcctaa cagcctctgc tatgtggaaa
     3781 cagcagaact ggacggacaa accaatttaa aatttaagat gtcacttgaa atcacagacc
     3841 agtacctcca aagagaagat acattggcta catttgatgg ttttattgaa tgtgaagaac
     3901 ccaataaccg actggataag tttacaggaa cactattttg gagaaacaca agttttcctt
     3961 tggatgctga taaaattttg ttacgtggct gtgtaattag gaacaccgat ttctgccacg
     4021 gcttagtcat ttttgcaggt gctgacacta aaataatgaa gaatagtggg aaaaccagat
     4081 ttaaaagaac taaaattgat tacttgatga actacatggt ttacacgatc tttgttgttc
     4141 ttattctgct ttctgctggt cttgccatcg gccatgctta ttgggaagca caggtgggca
     4201 attcctcttg gtacctctat gatggagaag acgatacacc ctcctaccgt ggattcctca
     4261 ttttctgggg ctatatcatt gttctcaaca ccatggtacc catctctctc tatgtcagcg
     4321 tggaagtgat tcgtcttgga cagagtcact tcatcaactg ggacctgcaa atgtactatg
     4381 ctgagaagga cacacccgca aaagctagaa ccaccacact caatgaacag ctcgggcaga
     4441 tccattatat cttctctgat aagacgggga cactcacaca aaatatcatg acctttaaaa
     4501 agtgctgtat caacgggcag atatatgggg accatcggga tgcctctcaa cacaaccaca
     4561 acaaaataga gcaagttgat tttagctgga atacatatgc tgatgggaag cttgcatttt
     4621 atgaccacta tcttattgag caaatccagt cagggaaaga gccagaagta cgacagttct
     4681 tcttcttgct cgcagtttgc cacacagtca tggtggatag gactgatggt cagctcaact
     4741 accaggcagc ctctcccgat gaaggtgccc tggtaaacgc tgccaggaac tttggctttg
     4801 ccttcctcgc caggacccag aacaccatca ccatcagtga actgggcact gaaaggactt
     4861 acaatgttct tgccattttg gacttcaaca gtgaccggaa gcgaatgtct atcattgtaa
     4921 gaaccccaga aggcaatatc aagctttact gtaaaggtgc tgacactgtt atttatgaac
     4981 ggttacatcg aatgaatcct actaagcaag aaacacagga tgccctggat atctttgcaa
     5041 atgaaactct tagaacccta tgcctttgct acaaggaaat tgaagaaaaa gaatttacag
     5101 aatggaataa aaagtttatg gctgccagtg tggcctccac caaccgggac gaagctctgg
     5161 ataaagtata tgaggagatt gaaaaagact taattctcct gggagctaca gctattgaag
     5221 acaagctaca ggatggagtt ccagaaacca tttcaaaact tgcaaaagct gacattaaga
     5281 tctgggtgct tactggagac aaaaaggaaa ctgctgaaaa tataggattt gcttgtgaac
     5341 ttctgactga agacaccacc atctgctatg gggaggatat taattctctt cttcatgcaa
     5401 ggatggaaaa ccagaggaat agaggtggcg tctacgcaaa gtttgcacct cctgtgcagg
     5461 aatctttttt tccacccggt ggaaaccgtg ccttaatcat cactggttct tggttgaatg
     5521 aaattcttct cgagaaaaag accaagagaa ataagattct gaagctgaag ttcccaagaa
     5581 cagaagaaga aagacggatg cggacccaaa gtaaaaggag gctagaagct aagaaagagc
     5641 agcggcagaa aaactttgtg gacctggcct gcgagtgcag cgcagtcatc tgctgccgcg
     5701 tcacccccaa gcagaaggcc atggtggtgg acctggtgaa gaggtacaag aaagccatca
     5761 cgctggccat cggagatggg gccaatgacg tgaacatgat caaaactgcc cacattggcg
     5821 ttggaataag tggacaagaa ggaatgcaag ctgtcatgtc gagtgactat tcctttgctc
     5881 agttccgata tctgcagagg ctactgctgg tgcatggccg atggtcttac ataaggatgt
     5941 gcaagttcct acgatacttc ttttacaaaa actttgcctt tactttggtt catttctggt
     6001 actccttctt caatggctac tctgcgcaga ctgcatacga ggattggttc atcaccctct
     6061 acaacgtgct gtacaccagc ctgcccgtgc tcctcatggg gctgctcgac caggatgtga
     6121 gtgacaaact gagcctccga ttccctgggt tatacatagt gggacaaaga gacttactat
     6181 tcaactataa gagattcttt gtaagcttgt tgcatggggt cctaacatcg atgatcctct
     6241 tcttcatacc tcttggagct tatctgcaaa ccgtagggca ggatggagag gcaccttccg
     6301 actaccagtc ttttgccgtc accattgcct ctgctcttgt aataacagtc aatttccaga
     6361 ttggcttgga tacttcttat tggacttttg tgaatgcttt ttcaattttt ggaagcattg
     6421 cactttattt tggcatcatg tttgactttc atagtgctgg aatacatgtt ctctttccat
     6481 ctgcatttca atttacaggc acagcttcaa acgctctgag acagccatac atttggttaa
     6541 ctatcatcct gactgttgct gtgtgcttac tacccgtcgt tgccattcga ttcctgtcaa
     6601 tgaccatctg gccatcagaa agtgataaga tccagaagca tcgcaagcgg ttgaaggcgg
     6661 aggagcagtg gcagcgacgg cagcaggtgt tccgccgggg cgtgtcaacg cggcgctcgg
     6721 cctacgcctt ctcgcaccag cggggctacg cggacctcat ctcctccggg cgcagcatcc
     6781 gcaagaagcg ctcgccgctt gatgccatcg tggcggatgg caccgcggag tacaggcgca
     6841 ccggggactc gtcgagatat ctagacccag ctttcttgta caaagtggtt gatctagagg
     6901 gcccgcggtt cgaactcgag gtaccgaatt cgtacccata cgacgtccca gactacgctt
     6961 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     7021 tggctcacaa ataccactga gatcgatctt tttccctctg ccaaaaatta tggggacatc
     7081 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     7141 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     7201 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     7261 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     7321 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     7381 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     7441 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     7501 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7681 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7741 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7801 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7861 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     7921 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7981 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     8041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8701 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9901 cctttttcaa t
//
