LOCUS       sequence-413043-        9733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B2 mutant E171Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pCAG/ATP8B2(E171Q)-HA
FEATURES             Location/Qualifiers
     source          1..9733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1939..2955
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2879..2901
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2963..2982
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3051..3075
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6687..6711)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6755..6781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6869..6888)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6934..6989
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6988..7007)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7350..7366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7350..7366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7363..7385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7374..7390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7398..7404,7405..7422,7423..7428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7443..7464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7523..7718
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7569..7704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7724..7858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7761..7780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7815..7834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7926..7943)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8097..8685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8177..8196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8856..9647,9648..9716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9479..9498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9717..9733,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg gggggggggg ggggcgggcg gggggggggg
     1801 ggggggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
     1861 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     1921 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
     1981 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     2041 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     2101 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     2161 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     2221 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     2281 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     2341 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     2401 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     2461 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     2521 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     2581 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     2641 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
     2701 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     2761 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     2821 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     2881 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     2941 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     3001 aaagaatttg ctagcgagct cgcggccgca gatctctagt taagctatca acaagtttgt
     3061 acaaaaaagc aggctcttta aaggaaccaa ttcagtcgac accatggcag tgtgtgcaaa
     3121 aaagcgcccc ccagaagaag aaaggagggc gcgggctaat gaccgagaat acaatgagaa
     3181 attccagtat gcgagtaact gcatcaagac ctccaagtac aatattctca ccttcctgcc
     3241 tgtcaacctc tttgagcagt tccaggaagt tgccaacact tacttcctgt tcctcctcat
     3301 tctgcagttg atcccccaga tctcttccct gtcctggttc accaccattg tgcctttggt
     3361 tcttgtcctc accatcacag ctgttaaaga tgccactgat gactatttcc gccacaagag
     3421 cgataaccag gtgaataacc gccagtctca ggtgctgatc aatggaatcc tccagcagga
     3481 gcagtggatg aatgtctgtg ttggtgatat tatcaagcta gaaaataacc agtttgtggc
     3541 ggcggatctc ctcctccttt ccagcagtga gccccatggg ctgtgttaca tagagacagc
     3601 agaacttgat ggccagacca acatgaaagt acgtcaggcg attccagtca cctcagaatt
     3661 gggagacatc agtaagcttg ccaagtttga cggtgaagtg atctgtgaac ctcccaacaa
     3721 caaactggac aaattcagcg gaaccctcta ctggaaggaa aataagttcc ctctgagcaa
     3781 ccagaacatg ctgctgcggg gctgtgtgct gcgaaacacc gagtggtgct tcgggctggt
     3841 catctttgca ggtcccgaca ctaagctgat gcaaaacagc ggcagaacaa agttcaaaag
     3901 aacgagtatc gatcgcctaa tgaataccct ggtgctctgg atttttggat tcctggtttg
     3961 catgggggtg atcctggcca ttggcaatgc catctgggag cacgaggtgg ggatgcgttt
     4021 ccaggtctac ctgccgtggg atgaggcagt ggacagtgcc ttcttctctg gcttcctctc
     4081 cttctggtcc tacatcatca tcctcaacac cgttgtgccc atttcactct atgtcagtgt
     4141 ggaggtcatc cgtctgggcc acagctactt catcaactgg gataagaaga tgttctgcat
     4201 gaagaagcgg acgcctgcag aagcccgcac caccacccta aacgaggagc tgggccaggt
     4261 ggagtacatc ttctccgaca agacgggcac cctcacccag aacatcatgg ttttcaacaa
     4321 gtgctccatc aatggccaca gctatggtga tgtgtttgac gtcctgggac acaaagctga
     4381 attgggagag aggcctgaac ctgttgactt ctccttcaat cctctggctg acaagaagtt
     4441 cttattttgg gaccccagcc tgctggaggc tgtcaagatc ggggaccccc acacgcatga
     4501 gttcttccgc ctcctttccc tgtgtcatac tgtcatgtca gaagaaaaga acgaaggaga
     4561 gctgtactac aaagctcagt ccccagatga gggggccctg gtcaccgcag ccaggaactt
     4621 tggttttgtt ttccgctctc gcacccccaa aacaatcacc gtccatgaga tgggcacagc
     4681 catcacctac cagctgctgg ccatcctgga cttcaacaac atccgcaagc ggatgtcggt
     4741 catagtgcgg aatccagagg ggaagatccg actctactgc aaaggggctg acactatcct
     4801 actggacaga ctgcaccact ccactcaaga gctgctcaac accaccatgg accaccttaa
     4861 tgagtacgca ggggaagggc tgaggaccct ggtgctggcc tacaaggatc tggatgaaga
     4921 gtactacgag gagtgggctg agcgacgcct ccaggccagc ctggcccagg acagccggga
     4981 ggacaggctg gctagcatct atgaggaggt tgagaacaac atgatgctgc tgggtgcaac
     5041 ggccattgag gacaaacttc agcaaggggt tccagagacc attgccctcc tgacactggc
     5101 caacatcaag atttgggtgc taaccggaga caagcaagag acggctgtga acatcggcta
     5161 ttcctgcaag atgctgacgg atgacatgac tgaggttttc atagtcactg gccatactgt
     5221 cctggaggtg cgggaggagc tcaggaaagc ccgggagaag atgatggact catcccgctc
     5281 tgtaggcaac ggcttcacct atcaggacaa gctttcttct tccaagctaa cttctgtcct
     5341 ggaggccgtt gctggggagt acgccctggt cataaatggt cacagcctgg cccacgcact
     5401 ggaggcagac atggagctgg agtttctgga gacagcgtgt gcctgcaaag ctgtcatctg
     5461 ctgccgggtg acccccttgc agaaggcaca ggtggtagaa ctggtcaaga agtacaagaa
     5521 ggctgtgacg cttgccattg gagacggagc caatgatgtc agcatgatca aaacggctca
     5581 cattggtgtg gggatcagtg ggcaggaagg gatccaggct gtcttggcct ccgattactc
     5641 cttctcccag ttcaagttcc tgcagcgcct cctgctggtg catgggcgct ggtcctacct
     5701 gcgaatgtgc aagtttcttt gctatttctt ctacaaaaac tttgctttca ccatggtcca
     5761 cttctggttt ggcttcttct gtggcttctc agcccagacc gtctatgacc agtatttcat
     5821 caccctgtat aacatcgtgt acacctccct gccagtcctg gctatggggg tctttgatca
     5881 ggatgtcccc gagcagcgga gcatggagta ccctaagctg tatgagccgg gccagctgaa
     5941 ccttctcttc aacaagcggg agttcttcat ctgcatcgcc cagggcatct acacctccgt
     6001 gctcatgttc ttcattccct atggggtgtt tgctgatgcc acccgggatg atggcactca
     6061 gctggctgac taccagtcct ttgcagtcac tgtggccaca tccttggtca ttgtggttag
     6121 cgtgcagatt gggctcgaca caggctactg gacggccatc aaccacttct tcatctgggg
     6181 aagccttgct gtttactttg ccatcctctt tgccatgcac agcaatgggc tcttcgacat
     6241 gtttcccaac cagttccggt ttgtggggaa tgcccagaac accttggccc agcccacggt
     6301 gtggctgacc attgtgctca ccacagtcgt ctgcatcatg cccgtggttg ccttccgatt
     6361 cctcaggctc aacctgaagc cggatctctc cgacacggtc cgctacacac agctcgtgag
     6421 gaagaagcag aaggcccagc accgctgcat gcggcgggtt ggccgcactg gctcccggcg
     6481 ctccggctat gccttctccc atcaggaggg cttcggggag ctcatcatgt ctggcaagaa
     6541 catgcggctg agctctctcg cgctctccag cttcaccacc cgctccagct ccagctggat
     6601 tgagagcctg cgcaggaaga agagtgacag tgccagtagc cccagtggcg gtgccgacaa
     6661 gcccctcaag ggctcgagat atctagaccc agctttcttg tacaaagtgg ttgatctaga
     6721 gggcccgcgg ttcgaactcg aggtaccgaa ttcgtaccca tacgacgtcc cagactacgc
     6781 ttaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     6841 cctggctcac aaataccact gagatcgatc tttttccctc tgccaaaaat tatggggaca
     6901 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     6961 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     7021 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     7081 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     7141 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     7201 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     7261 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     7321 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7381 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7441 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7501 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     7561 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7621 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7681 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     7741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     7861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9721 ttcctttttc aat
//
