LOCUS       sequence-413044-        9735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B4 mutant E170Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pCAG/ATP8B4(E170Q)-HA
FEATURES             Location/Qualifiers
     source          1..9735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1928..2944
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2868..2890
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2952..2971
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3040..3064
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6689..6713)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6757..6783
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6871..6890)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6936..6991
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6990..7009)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7352..7368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7352..7368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7365..7387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7376..7392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7400..7406,7407..7424,7425..7430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7445..7466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7525..7720
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7571..7706
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7726..7860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7763..7782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7817..7836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7928..7945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8099..8687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8179..8198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8858..9649,9650..9718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9481..9500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9719..9735,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acctcctatg tatcgatcag ttatgctgtc gtgagggggg
     1741 gggggggggg ggggaggggg gggggggggg gggggggcgg ggcggggggg gggggggggg
     1801 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     1861 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     1921 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     1981 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     2041 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     2101 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     2161 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     2221 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     2281 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     2341 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     2401 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     2461 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     2521 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     2581 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     2641 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     2701 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     2761 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     2821 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     2881 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     2941 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaatttgc
     3001 tagcgagctc gcggccgcag atctctagtt aagctatcaa caagtttgta caaaaaagca
     3061 ggctctttaa aggaaccaat tcagtcgact ggatccacca tgttctgcag tgaaaagaaa
     3121 ttgcgtgaag tggaacggat agtgaaagcc aatgaccgtg aatataatga aaagttccag
     3181 tatgcggata atcgtatcca cacatcgaaa tataatattc tcaccttctt gccaattaat
     3241 ttatttgaac agttccaaag agtggcaaat gcctattttc tttgccttct gattttacag
     3301 ctaattccag aaatttcctc cttgacctgg tttaccacca ttgtgccttt ggtcctggtg
     3361 ataactatga cagctgtcaa agatgccaca gatgactatt ttcgccacaa gagtgataat
     3421 caagtgaata atcggcagtc tgaagtgctc atcaacagca aactgcagaa tgaaaaatgg
     3481 atgaatgtca aagtgggaga catcattaaa ttagaaaata accaatttgt tgctgctgat
     3541 ttacttctcc tatcaagtag tgagccacat ggtctctgtt atgttgaaac tgctgagctt
     3601 gatgggcaaa cgaacctaaa agtccgccat gcactatcag ttacttcaga acttggagca
     3661 gatatcagca gacttgcagg gtttgatggg attgttgtct gtgaggtgcc taacaacaag
     3721 ttagataaat tcatgggaat cctttcttgg aaagacagca agcattccct caacaatgag
     3781 aagataatcc tgagaggctg catcctgaga aataccagct ggtgttttgg aatggttatt
     3841 tttgcaggtc ctgacactaa actaatgcag aatagtggta agacaaagtt taaaaggaca
     3901 agcattgata gattgatgaa tactctagta ctatggattt ttgggtttct gatatgcttg
     3961 ggaattattc ttgcaatagg aaattcaatc tgggagagtc aaactgggga ccaattcaga
     4021 actttcctct tttggaatga aggagagaag agctctgtgt tctccggatt cttaacattc
     4081 tggtcatata ttattattct caatacagtt gtacccattt ccttatatgt gagtgtggaa
     4141 gtaattcgtc taggacacag ttattttata aactgggacc ggaagatgta ttattctcga
     4201 aaagcaatac ctgcagtggc tcgaacgacc acgctcaatg aggaactggg gcagattgag
     4261 tacattttct ccgacaaaac gggtaccctc actcaaaaca tcatgacctt taaaagatgt
     4321 tccattaatg ggagaatcta tggtgaagta catgatgacc tggatcagaa gacagaaata
     4381 actcaggaaa aagagcctgt ggatttctca gtcaaatctc aagcggatag agaatttcag
     4441 ttctttgacc accatctgat ggaatccatt aaaatgggtg atcccaaagt tcatgaattc
     4501 cttaggttac ttgctctctg ccacactgta atgtcagaag agaatagcgc aggagagctg
     4561 atttaccaag ttcagtcacc tgatgaaggg gctctagtga ctgccgctag aaattttggg
     4621 ttcattttta aatcccggac cccagagacc ataacaatag aagaattggg aacactagtt
     4681 acttatcaat tacttgcctt tttggatttc aacaacacca gaaaaaggat gtctgtcata
     4741 gttcgaaacc cagaaggaca gataaagctt tattccaaag gagcagatac tattctgttt
     4801 gaaaaacttc atccttccaa tgaagtcctt ttgtctttga cgtcagacca cctcagtgaa
     4861 tttgcagggg aaggccttcg gaccttggcc atcgcataca gagacctgga tgacaagtac
     4921 tttaaagagt ggcataagat gcttgaagat gcgaatgctg ccacagaaga gagggatgaa
     4981 cgaatagctg agctatatga agaaattgaa agagatttga tgctactagg tgccactgct
     5041 gtagaagata agttacagga gggtgttatt gaaacagtta caagtttatc actagccaat
     5101 attaagatct gggtcctaac aggagacaaa caagaaactg ccatcaacat cggttatgcc
     5161 tgcaacatgc tgactgacga catgaatgat gtgtttgtga tagcagggaa taatgctgtg
     5221 gaagtgagag aagaactcag gaaagcaaaa caaaatttgt ttggacaaaa cagaaatttt
     5281 tccaatggcc atgtagtttg tgaaaaaaag cagcagctgg agttggattc tattgtagaa
     5341 gaaaccataa caggagatta tgccttaatc ataaatggcc acagtttggc tcatgcccta
     5401 gaaagtgatg tcaagaatga tctcctagaa cttgcttgca tgtgtaagac tgtaatttgc
     5461 tgcagggtca ctccactcca gaaagcccaa gtggtagagc tggtgaagaa gtacagaaat
     5521 gctgttactt tggccattgg tgatggagcc aatgatgtca gcatgattaa aagtgctcac
     5581 attggtgttg gcatcagcgg ccaggaagga ttgcaagcag tcttagccag cgactattca
     5641 tttgcacagt ttagatatct ccaaaggctt ctccttgttc atggaaggtg gtcttatttc
     5701 cgaatgtgca aattcttatg ctatttcttc tataagaatt ttgcatttac acttgtgcat
     5761 ttctggtttg gtttcttctg tggtttctca gcccagactg tttatgacca gtggttcatc
     5821 acccttttta acattgttta cacatcactg cctgttttag ccatggggat ttttgaccag
     5881 gatgtgagtg accagaacag cgtggactgt ccccagctct acaaaccagg acagctgaat
     5941 ctgcttttta acaagcgtaa atttttcatt tgcgtgttgc atggaatcta cacctcatta
     6001 gtccttttct tcatccccta tggggccttt tacaacgtgg ctggagaaga tgggcaacat
     6061 attgctgact accagtcctt tgcagttacc atggccacat ctttggtcat tgtggtcagt
     6121 gtgcagatag ccttggatac cagttactgg actttcatta atcacgtctt catctggggg
     6181 agcattgcca tttatttctc cattttattt acaatgcaca gtaatggcat ctttggcatc
     6241 ttcccaaacc agtttccatt tgttggtaat gcacgacatt ccctgaccca gaagtgcatc
     6301 tggcttgtaa ttctcttaac aacagtggct tcagttatgc cagtggtggc attcagattt
     6361 ttgaaggtgg atttataccc aaccctgagt gatcagatcc gccggtggca gaaggctcaa
     6421 aagaaggcaa ggcctccaag tagccgaagg cctcggaccc gcaggtcaag ctcaagaagg
     6481 tctggatatg cttttgctca ccaagaaggc tatggagagc ttatcacatc tggaaaaaat
     6541 atgcgagcta aaaatccacc cccaacatca gggctggaaa agacacatta taatagcact
     6601 agctggattg aaaatttatg taagaaaacc acagacaccg tgagcagctt tagccaggat
     6661 aaaacagtga aactctcgag atatctagac ccagctttct tgtacaaagt ggttgatcta
     6721 gagggcccgc ggttcgaact cgaggtaccg aattcgtacc catacgacgt cccagactac
     6781 gcttaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     6841 gccctggctc acaaatacca ctgagatcga tctttttccc tctgccaaaa attatgggga
     6901 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     6961 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     7021 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     7081 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     7141 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     7201 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     7261 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     7321 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7381 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7441 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7501 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     7561 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7621 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7681 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     7741 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7801 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     7861 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7921 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7981 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9721 tcttcctttt tcaat
//
