LOCUS       sequence-413045-        9618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP11A mutant E186Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pCAG/ATP11A(E186Q)-HA
FEATURES             Location/Qualifiers
     source          1..9618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1982..2998
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2922..2944
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3006..3025
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3094..3118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6572..6596)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6640..6666
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6754..6773)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6819..6874
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6873..6892)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7235..7251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7235..7251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7248..7270)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7259..7275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7283..7289,7290..7307,7308..7313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7328..7349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7408..7603
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7454..7589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7609..7743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7646..7665)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7700..7719
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7811..7828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7982..8570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8062..8081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8741..9532,9533..9601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9364..9383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9602..9618,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg gggcgcccgg gggggggggg gggggggggg
     1801 gggggggggg gggggggggg gggggggggg gggggggggg gcgggggggg gcggggggcg
     1861 ggggggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     1921 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     1981 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     2041 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     2101 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     2161 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     2221 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     2281 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     2341 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     2401 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     2461 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     2521 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     2581 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     2641 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     2701 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     2761 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     2821 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     2881 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     2941 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     3001 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat ttgctagcga
     3061 gctcgcggcc gcagatctct agttaagcta tcaacaagtt tgtacaaaaa agcaggctct
     3121 ttaaaggaac caattcagtc gactggatcc accatggact gcagcctcgt gcggacgctc
     3181 gtgcacagat actgtgcagg agaagagaat tgggtggaca gcaggaccat ctacgtggga
     3241 cacagggagc cacctccggg cgcagaggcc tacatcccac agagataccc agacaacagg
     3301 atcgtctcgt ccaagtacac attttggaac tttataccca agaatttatt tgaacaattc
     3361 agaagagtag ccaactttta tttccttatc atatttctgg tgcagttgat tattgataca
     3421 cccacaagtc cagtgacaag cggacttcca ctcttctttg tcattactgt gacggctatc
     3481 aaacagggtt atgaagactg gcttcgacat aaagcagaca atgccatgaa ccagtgtcct
     3541 gttcatttca ttcagcacgg caagctcgtt cggaaacaaa gtcgaaagct gcgagttggg
     3601 gacattgtca tggttaagga ggacgagacc tttccctgcg acttgatctt cctttccagc
     3661 aaccggggag atgggacgtg ccacgtcacc accgccagct tggatggaca atccagccat
     3721 aaaacgcatt acgcagtcca ggacaccaaa ggcttccaca cagaggagga tatcggcgga
     3781 cttcacgcca ccatcgagtg tgagcagccc cagcccgacc tctacaagtt cgtgggtcgc
     3841 atcaacgttt acagtgacct gaatgacccc gtggtgaggc ccttaggatc ggaaaacctg
     3901 ctgcttagag gagctacact gaagaacact gagaaaatct ttggtgtggc tatttacacg
     3961 ggaatggaaa ccaagatggc attaaattat caatcaaaat ctcagaagcg atctgccgtg
     4021 gaaaaatcga tgaatgcgtt cctcattgtg tatctctgca ttctgatcag caaagccctg
     4081 ataaacactg tgctgaaata cgtgtggcag agtgagccct ttcgggatga gccgtggtat
     4141 aatcagaaaa cggagtcgga aaggcagagg aatctgttcc tcaaggcatt cacggacttc
     4201 ctggccttca tggtcctctt taactacatc atccctgtgt ccatgtacgt cacggtcgag
     4261 atgcagaagt tcctcggctc ttacttcatc acctgggacg aagacatgtt tgacgaggag
     4321 actggcgagg ggcctctggt gaacacgtcg gacctcaatg aagagctggg acaggtggag
     4381 tacatcttca cagacaagac cggcaccctc acggaaaaca acatggagtt caaggagtgc
     4441 tgcatcgaag gccatgtcta cgtgccccac gtcatctgca acgggcaggt cctcccagag
     4501 tcgtcaggaa tcgacatgat tgactcgtcc cccagcgtca acgggaggga gcgcgaggag
     4561 ctgtttttcc gggccctctg tctctgccac accgtccagg tgaaagacga tgacagcgta
     4621 gacggcccca ggaaatcgcc ggacgggggg aaatcctgtg tgtacatctc atcctcgccc
     4681 gacgaggtgg cgctggtcga aggtgtccag agacttggct ttacctacct aaggctgaag
     4741 gacaattaca tggagatatt aaacagggag aaccacatcg aaaggtttga attgctggaa
     4801 attttgagtt ttgactcagt cagaaggaga atgagtgtaa ttgtaaaatc tgctacagga
     4861 gaaatttatc tgttttgcaa aggagcagat tcttcgatat tcccccgagt gatagaaggc
     4921 aaagttgacc agatccgagc cagagtggag cgtaacgcag tggaggggct ccgaactttg
     4981 tgtgttgctt ataaaaggct gatccaagaa gaatatgaag gcatttgtaa gctgctgcag
     5041 gctgccaaag tggcccttca agatcgagag aaaaagttag cagaagccta tgagcaaata
     5101 gagaaagatc ttactctgct tggtgctaca gctgttgagg accggctgca ggagaaagct
     5161 gcagacacca tcgaggccct gcagaaggcc gggatcaaag tctgggttct cacgggagac
     5221 aagatggaga cggccgcggc cacgtgctac gcctgcaagc tcttccgcag gaacacgcag
     5281 ctgctggagc tgaccaccaa gaggatcgag gagcagagcc tgcacgacgt cctgttcgag
     5341 ctgagcaaga cggtcctgcg ccacagcggg agcctgacca gagacaacct ctccggactt
     5401 tcagcagata tgcaggacta cggtttaatt atcgacggag ctgcactgtc tctgataatg
     5461 aagcctcgag aagacgggag ttccggcaac tacagggagc tcttcctgga aatctgccgg
     5521 agctgcagcg cggtgctctg ctgccgcatg gcgcccttgc agaaggctca gattgttaaa
     5581 ttaatcaaat tttcaaaaga gcacccaatc acgttagcaa ttggcgatgg tgcaaatgat
     5641 gtcagcatga ttctggaagc gcacgtgggc ataggtgtca tcggcaagga aggccgccag
     5701 gctgccagga acagcgacta tgcaatccca aagtttaagc atttgaagaa gatgctgctt
     5761 gttcacgggc atttttatta cattaggatc tctgagctcg tgcagtactt cttctataag
     5821 aacgtctgct tcatcttccc tcagttttta taccagttct tctgtgggtt ttcacaacag
     5881 actttgtacg acaccgcgta tctgaccctc tacaacatca gcttcacctc cctccccatc
     5941 ctcctgtaca gcctcatgga gcagcatgtt ggcattgacg tgctcaagag agacccgacc
     6001 ctgtacaggg acgtcgccaa gaatgccctg ctgcgctggc gcgtgttcat ctactggacg
     6061 ctcctgggac tgtttgacgc actggtgttc ttctttggtg cttatttcgt gtttgaaaat
     6121 acaactgtga caagcaacgg gcagatattt ggaaactgga cgtttggaac gctggtattc
     6181 accgtgatgg tgttcacagt tacactaaag cttgcattgg acacacacta ctggacttgg
     6241 atcaaccatt ttgtcatctg ggggtcgctg ctgttctacg ttgtcttttc acttctctgg
     6301 ggaggagtga tctggccgtt cctcaactac cagaggatgt actacgtgtt catccagatg
     6361 ctgtccagcg ggcccgcctg gctggccatc gtgctgctgg tgaccatcag cctccttccc
     6421 gacgtcctca agaaagtcct gtgccggcag ctgtggccaa cagcaacaga gagagtccag
     6481 actaagagcc agtgcctttc tgtcgagcag tcaaccatct ttatgctttc tcagacttcc
     6541 agcagcctga gtttcgcgtc gagatatcta gacccagctt tcttgtacaa agtggttgat
     6601 ctagagggcc cgcggttcga actcgaggta ccgaattcgt acccatacga cgtcccagac
     6661 tacgcttaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     6721 aatgccctgg ctcacaaata ccactgagat cgatcttttt ccctctgcca aaaattatgg
     6781 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     6841 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     6901 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     6961 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     7021 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     7081 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     7141 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     7201 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7261 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7321 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7381 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     7441 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7501 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7561 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     7621 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7681 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7741 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7801 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7861 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8401 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8641 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8701 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8761 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8821 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8881 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8941 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9001 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9061 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9121 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9181 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9241 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9301 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9361 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9421 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9481 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9541 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9601 tactcttcct ttttcaat
//
