LOCUS       sequence-413067-        5830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes the human vasopressin receptor 2, tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Di Giglio MG, Muttenthaler M, Harpsoe K, Liutkeviciute Z, Keov P, 
            Eder T, Rattei T, Arrowsmith S, Wray S, Marek A, Elbert T, Alewood 
            PF, Gloriam DE, Gruber CW
  TITLE     Development of a human vasopressin V(1a)-receptor antagonist from an
            evolutionary-related insect neuropeptide.
  JOURNAL   Sci Rep. 2017 Feb 1;7:41002. doi: 10.1038/srep41002.
  PUBMED    28145450
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-02-1"; volume: "7:41002. doi"; pages: " 
            10.1038/srep41002"
COMMENT     Sequence Label: V2R-GFP
FEATURES             Location/Qualifiers
     source          1..5830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1770..1779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1776..1778,1779..1781,1782..2495)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1821..1842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2082..2101)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2429..2450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2618..2739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2655..2674)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2709..2728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2746..3201)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2883..2904)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3095..3114
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3228..3332
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3334..3691
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3334..3525
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3357..3377)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3542..3677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3726..4520
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3726..4520
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3780..3799)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4390..4409
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4708..4727)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4752..4799
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5128..5716
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5617..5636
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct cggatcgatc ccgggaattc ctgccccagc cccaccatgc
      661 tcatggcgtc caccacttcc gctgtgcctg ggcatccctc tctgcccagc ctgcccagca
      721 acagcagcca ggagaggcca ctggacaccc gggacccgct gctagcccgg gcggagctgg
      781 cgctgctctc catagtcttt gtggctgtgg ccctgagcaa tggcctggtg ctggcggccc
      841 tagctcggcg gggccggcgg ggccactggg cacccataca cgtcttcatt ggccacttgt
      901 gcctggccga cctggccgtg gctctgttcc aagtgctgcc ccagctggcc tggaaggcca
      961 ccgaccgctt ccgtgggcca gatgccctgt gtcgggccgt gaagtatctg cagatggtgg
     1021 gcatgtatgc ctcctcctac atgatcctgg ccatgacgct ggaccgccac cgtgccatct
     1081 gccgtcccat gctggcgtac cggcatggaa gtggggctca ctggaaccgg ccggtgctag
     1141 tggcttgggc cttctcgctc cttctcagcc tgccccagct cttcatcttc gcccagcgca
     1201 acgtggaagg tggcagcggg gtcactgact gctgggcctg ctttgcggag ccctggggcc
     1261 gtcgcaccta tgtcacctgg attgccctga tggtgttcgt ggcacctacc ctgggtatcg
     1321 ccgcctgcca ggtgctcatc ttccgggaga ttcatgccag tctggtgcca gggccatcag
     1381 agaggcctgg ggggcgccgc aggggacgcc ggacaggcag ccccggtgag ggagcccacg
     1441 tgtcagcagc tgtggccaag actgtgagga tgacgctagt gattgtggtc gtctatgtgc
     1501 tgtgctgggc acccttcttc ctggtgcagc tgtgggccgc gtgggacccg gaggcacctc
     1561 tggaaggggc gccctttgtg ctactcatgt tgctggccag cctcaacagc tgcaccaacc
     1621 cctggatcta tgcatctttc agcagcagcg tgtcctcaga gctgcgaagc ttgctctgct
     1681 gtgcccgggg acgcacccca cccagcctgg gtccccaaga tgagtcctgc accaccgcca
     1741 gctcctccct ggccaaggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1861 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1981 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2041 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2221 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2281 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2461 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat
     2521 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2581 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2641 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2701 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     2761 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     2821 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2881 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     2941 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3001 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3061 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3121 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3181 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3241 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3301 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     3361 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3421 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3481 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3541 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3601 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3661 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     3721 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     3781 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     3841 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     3901 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3961 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4021 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4081 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4141 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4201 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     4261 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4321 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4381 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4441 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4501 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     4561 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     4621 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     4681 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     4741 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     4801 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4861 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4921 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     4981 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5041 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5101 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5161 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5221 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5281 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5341 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5401 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5461 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5521 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5581 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5641 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5701 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5761 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5821 cgccatgcat
//
