LOCUS       sequence-413071-       10198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac vector backbone encoding doxycycline-inducible expression 
            of membrane-localized catalytic domain of SOS (SOScat).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Underhill EJ, Toettcher JE
  TITLE     Control of gastruloid patterning and morphogenesis by the Erk and 
            Akt signaling pathways.
  JOURNAL   Development. 2023 Aug 15;150(16):dev201663. doi: 10.1242/dev.201663.
            Epub 2023 Aug 17.
  PUBMED    37590131
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2023-08-15"; volume: "150(16):dev201663. 
            doi"; pages: " 10.1242/dev.201663. Epub 2023 Aug 17"
COMMENT     Sequence Label: Dox-SOScat
FEATURES             Location/Qualifiers
     source          1..10198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..770
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             780..836
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             837..1583
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    1688..2276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2159..2170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   2519..2581
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2693..2712)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2812..2834
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2872..2890)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2958..3062
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3063..3131,3132..3923)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3281..3300)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4094..4682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4583..4602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4836..4853
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4970..4991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5006..5011,5012..5029,5030..5036)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5044..5060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5049..5071
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5068..5084
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5068..5084
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5119..5153
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5536..5914
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    5544..5562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5580..5598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5616..5634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5652..5670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5688..5706
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5724..5742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5760..5778
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5823..5847
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5960..5984
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5990..6010
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             6038..6736
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8343..8931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8814..8825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    9176..9194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9197..9215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          9260..10198
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10155..10175
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gtgtcgtgag gggatctgga gctctcgaga attctcacgc gtcaagtgga gcaaggcagg
       61 tggacagtgg atccatggtg agcgagctga ttaaggagaa catgcccatg aagctgtaca
      121 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
      181 acgagggcac ccagaccatg agaatcaagg tcgtcgaggg cggccctctc cccttcgcct
      241 tcgacatcct ggctaccagc ttcatgtacg gcagcagaac cttcatcaag caccctccgg
      301 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
      361 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
      421 tcatctacaa cgtcaaggtt agaggggtga acttcccagc caacggccct gtgatgcaga
      481 agaaaacact cggctgggag gcctccaccg agacgatgta ccccgctgac ggcggcctgg
      541 aaggcgcatg tgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaaccttg
      601 agaccacata cagatccaag aaacccgcta cgaacctcaa gatgcccggc gtctacaacg
      661 tggaccacag actggaaaga atcaaggagg ccgacgatga gacctacgtc gagcagcacg
      721 aggtggctgt ggccagatac tctactggtg gcgctggtga tggaggtaaa ggaagcggag
      781 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatga
      841 gccggctgga caagagcaaa gtgatcaaca gcgccctgga actgctgaac ggcgtgggca
      901 tcgagggcct gaccacccgg aagctggccc agaaactggg cgtggaacag cccaccctgt
      961 actggcacgt gaagaacaag cgggccctgc tggacgccct gcccatcgag atgctggacc
     1021 ggcaccacac ccacagctgc cctctggaag gcgagagctg gcaggacttc ctgcggaaca
     1081 acgccaagag ctacagatgc gccctgctga gccaccggga cggcgccaaa gtgcacctgg
     1141 gcaccagacc caccgagaag cagtacgaga cactggaaaa ccagctggcc ttcctgtgcc
     1201 agcagggctt cagcctggaa aacgccctgt acgccctgag cgccgtgggc cacttcaccc
     1261 tgggctgcgt gctggaagaa caggaacacc aggtcgccaa agaggaaaga gagacaccca
     1321 ccaccgacag catgcccccc ctgctgaagc aggccatcga gctgttcgac agacagggcg
     1381 ccgagcccgc cttcctgttc ggcctggaac tgatcatctg cggcctcgag aagcagctga
     1441 agtgcgagag cggcggaccc accgacgccc tggacgactt cgacctggac atgctgcccg
     1501 ccgatgccct ggatgatttt gatctggata tgctccctgc tgacgctctc gatgacttcg
     1561 atctcgatat gctgccaggc tagcctgcag gcggccgcaa ctcccacctg caacatgcgt
     1621 gactgactga ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     1681 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1741 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1801 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1861 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1921 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1981 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2041 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2101 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2161 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2221 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2281 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgatact
     2341 agttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat
     2401 aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat
     2461 aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca
     2521 tgcgtcaatt ttacgcatta ttatctttaa cgtacgtcac aatatgatta tctttctagg
     2581 gttaatctag tatacgcgta tgcggccgct taattaatcc atggcgcctg atgcggtatt
     2641 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     2701 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     2761 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2821 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     2881 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     2941 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3001 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3061 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3121 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3181 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3241 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3301 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3361 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3421 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3481 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3541 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3601 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3661 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3721 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3781 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3841 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3901 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3961 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4021 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4081 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4141 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4201 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4261 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4321 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4381 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4441 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4501 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4561 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4621 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4681 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4741 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4801 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4861 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4921 ggcacgacag gtttcccgac tggaaagcgg gcagtgatcg caacgcaatt aatgtgagtt
     4981 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5041 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccgggg
     5101 cgcgccattc tagattaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt
     5161 gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc
     5221 agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt
     5281 gaactataac gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag
     5341 atttaactca tacgataatt atattgttat ttcatgttct acttacgtga taacttatta
     5401 tatatatatt ttcttgttat agatatcaac tagaatgcta gcatgggccc agatctccga
     5461 ggggacccga caggcccgca gggacagcag agatccagtt tggatcgatc gatcacgaga
     5521 ctagcctcga gagttggctt tactccctat cagtgataga gaacgtatga agagtttact
     5581 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     5641 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     5701 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     5761 ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc
     5821 agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat
     5881 accaactttc cgtaccactt cctaccctcg taaaccggtt cgtcgactag tccagtgtgg
     5941 tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccacca tgggttcctc
     6001 caaatccaag cccaaggacg catctcagcg acgtcgcatg agcgagctga ttaaggagaa
     6061 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     6121 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     6181 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     6241 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     6301 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     6361 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     6421 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     6481 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     6541 ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa
     6601 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     6661 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     6721 ggggcacaag cttaatagtg ctggtggtga attcatgagt gctggtggtg aattcatgca
     6781 accactgaga ttaccaagtc ctgaagtata tcgttttgta gtaaaagact ctgaggaaaa
     6841 cattgttttt gaagacaact tgcaaagtag aagtggcatc cccattatta aaggaggaac
     6901 tgtagtgaaa ttaattgaaa ggttaacata tcatatgtat gcagatccca attttgttcg
     6961 tacttttctt accacatatc gttcattttg taaaccacag gaattgctga gcttactgat
     7021 tgaacggttt gaaattccag agccagaacc tactgacgca gacaaattgg caatagagaa
     7081 aggcgagcag ccaatcagtg cagaccttaa aagatttcgc aaggaatatg tccaaccagt
     7141 acaacttagg atcttaaatg tatttcggca ttgggttgaa catcattttt atgactttga
     7201 aagagacttg gaattgcttg aaagactaga atccttcatt tcaagtgtaa gagggaaagc
     7261 tatgaaaaaa tgggtagagt caattgctaa gatcatcagg aggaagaagc aagctcaggc
     7321 aaacggagta agccataata ttacctttga aagtccacct ccaccaattg aatggcatat
     7381 cagcaaacca ggacagtttg aaacatttga tctcatgaca cttcatccaa tagaaattgc
     7441 acgtcagctg acacttttgg agtctgatct ttacaggaaa gttcaaccgt ctgaacttgt
     7501 agggagtgtg tggaccaaag aagataaaga aataaattct ccaaatttat taaaaatgat
     7561 tcgccatacc acaaatctca ccctctggtt tgaaaaatgc attgtggaag cagaaaattt
     7621 tgaagaacgg gtggcagtac taagtagaat tatagaaatt ctgcaagttt ttcaagattt
     7681 gaataatttc aatggcgtat tggagatagt cagtgcagta aattcagtgt cagtatacag
     7741 actagaccat acctttgagg cactgcagga aaggaaaagg aaaattttgg acgaagctgt
     7801 ggaattaagt caagatcact ttaaaaaata cctagtaaaa cttaagtcaa tcaatccacc
     7861 ttgtgtgcct ttttttggaa tatatttaac aaatattctg aagaccgaag aagggaataa
     7921 tgatttttta aaaaagaaag ggaaagattt aatcaatttc agtaagagga ggaaagtagc
     7981 tgaaattact ggagaaattc agcagtatca gaatcagcct tactgtttac ggatagaacc
     8041 agatatgagg agattctttg aaaaccttaa ccccatggga agtgcatctg aaaaagagtt
     8101 tacagattat ttgttcaaca agtcactaga aattgaacct cgaaactgca aacagccacc
     8161 tcgatttcct aggaaatcaa ctttttcctt aaaatctcct ggaataaggc ctaacacagg
     8221 ccgacatggc tctacctgac ctgcaggggc cgcaactccc acctgcaaca tgcgtgactg
     8281 actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
     8341 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     8401 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     8461 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     8521 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     8581 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     8641 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8701 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8761 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8821 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8881 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgcccgc
     8941 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ggctccggtg
     9001 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     9061 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     9121 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca ggatctccct
     9181 atcagtgata gagatctccc tatcagtgat agagatcgtc gacgttcttt ttcgcaacgg
     9241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     9301 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     9361 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     9421 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     9481 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     9541 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     9601 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     9661 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     9721 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     9781 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     9841 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     9901 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     9961 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
    10021 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
    10081 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttaagtttgg
    10141 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcag
//
