LOCUS       sequence-413114-       11632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acetylation mutant for human raptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Son SM, Park SJ, Lee H, Siddiqi F, Lee JE, Menzies FM, Rubinsztein 
            DC
  TITLE     Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A.
  JOURNAL   Cell Metab. 2019 Jan 8;29(1):192-201.e7. doi: 
            10.1016/j.cmet.2018.08.013. Epub 2018 Sep 6.
  PUBMED    30197302
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2018.08.013"; journalName: "Cell Metab"; date: 
            "2019-01-8- 8"; volume: "29"; issue: "1"; pages: "192-201.e7"
COMMENT     Sequence Label: pRK5-HA-YFP-Raptor K840R
FEATURES             Location/Qualifiers
     source          1..11632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        21..400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        401..604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     555..575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     601..625
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        835..853
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     835..852
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     866..885
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      970..979
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             982..1008
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1039..1048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1045..1047,1048..1050,1051..1761)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1090..1111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1351..1370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1698..1719
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      7939..7948
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7952..8086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7989..8008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8043..8062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        8155..8512
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8155..8346
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8178..8198)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8363..8498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8532..8549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8532..8548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8541..8563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8761..9216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8848..8867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9058..9079
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9233..9252)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9352..9374
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9412..9430)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9498..9602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9603..9671,9672..10463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9821..9840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10634..11222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11123..11142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11376..11393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11510..11531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11546..11551,11552..11569,11570..11576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11584..11600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11589..11611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11608..11624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11608..11624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattaattc gagctcgccc gacattgatt attgactagt tattaatagt aatcaattac
       61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      601 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      661 tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat
      721 tccccgtgcc aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc
      781 ttcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      841 gtgacactat agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc
      901 caactgcacc tcggttctat cgattgaatt cccgaaccga cagtcggtct cttcaccaag
      961 gccattcgcg ccaccatggg ctacccctat gatgtgcctg actacgcaga tcgcaatggt
     1021 ggtggtggtg gaccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1081 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1141 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1201 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     1261 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1321 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1381 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1441 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1501 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1561 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1621 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaaactgag caaagacccc
     1681 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1741 ggcatggacg agctgtacaa gtccggactc agatctggtg gagggcctgg tggaggtggg
     1801 cctggaggtg gaggacctgg tggtggtggg tcgacgatgg agtccgaaat gctgcaatcg
     1861 cctcttctgg gcctggggga ggaagatgag gctgatctta cagactggaa cctacctttg
     1921 gcttttatga aaaagaggca ctgtgagaaa attgaaggct ccaaatcctt agctcagagc
     1981 tggaggatga aggatcggat gaagacagtc agtgttgcct tagttttgtg cctgaatgtt
     2041 ggtgtggacc ctcccgatgt ggtgaagacc acgccctgtg cacgcttgga atgctggatc
     2101 gatcctctgt cgatgggtcc tcagaaagct ctggaaacca tcggtgcaaa tttacagaag
     2161 cagtacgaga actggcagcc aagggcccgg tacaagcaga gccttgaccc aactgtggat
     2221 gaagtcaaga agctctgcac gtccttacgt cgcaacgcca aggaggagcg agtcctcttt
     2281 cactacaatg gccacggggt gccccggccc acagtcaacg gggaggtctg ggtcttcaac
     2341 aagaactaca cgcagtacat ccctctgtcc atatatgacc tgcagacgtg gatgggcagc
     2401 ccgtcgatct tcgtctacga ctgctccaat gctggcttga tcgtcaagtc cttcaagcag
     2461 ttcgcactac agcgggagca ggagctggag gtagctgcaa tcaacccaaa tcaccctctt
     2521 gctcagatgc ctttgcctcc gtcgatgaaa aactgcatcc agctggcagc ctgcgaggcc
     2581 accgagctgc tgcccatgat ccccgacctc ccggctgacc tattcacctc ctgcctcacc
     2641 acccccatca agatcgccct gcgctggttt tgcatgcaga aatgtgtcag tctggtgcct
     2701 ggcgtcacac tggatttgat agaaaagatc cctggccgcc tgaacgacag gaggacgccc
     2761 ctgggtgaac tgaactggat cttcacagcc atcacagaca ccatcgcgtg gaacgtgctc
     2821 ccccgggatc tcttccaaaa gctcttcaga caggacttgc tggtggctag tctgtttcga
     2881 aattttttat tggcggaaag gattatgagg tcgtataact gcactcccgt cagcagcccg
     2941 cgtctgccgc ccacgtacat gcacgccatg tggcaagcct gggacctggc tgttgacatc
     3001 tgtctgtctc agctgccgac gatcatcgag gaaggcactg cgtttcggca cagcccgttc
     3061 ttcgccgagc agctgaccgc attccaggtg tggctcacca tgggcgtgga gaaccgaaac
     3121 ccacccgaac agctgcccat cgtcctgcag gtgctgttaa gccaagtgca ccggctgaga
     3181 gcattggact tgcttggaag atttttggac ctgggtccct gggcagtgag cctggccttg
     3241 tctgtcggca tcttccccta cgtgctgaag ctgctccaga gctcggcccg agagctgcgg
     3301 ccacttctcg ttttcatctg ggccaagatc ctcgcagtgg acagctcgtg ccaggcggac
     3361 ctcgtgaagg acaacggcca caagtacttc ctgtcggtcc tggcggaccc ctacatgcca
     3421 gctgaacacc ggaccatgac ggctttcatt ctcgccgtga tcgtcaacag ctatcacacg
     3481 gggcaggaag cctgccttca gggaaacctc attgccatct gcctggagca gctcaacgac
     3541 ccgcacccct tgctgcgcca gtgggtggcc atctgcctcg gcaggatctg gcagaacttc
     3601 gactcggcga ggtggtgcgg cgtgagggac agcgctcatg agaagctcta cagcctcctc
     3661 tccgacccca ttcccgaggt ccgctgcgca gcggtcttcg cccttggcac gttcgtgggc
     3721 aactctgcag agaggacgga ccactccacc accatcgacc acaacgtggc catgatgctg
     3781 gcccagctgg tcagcgacgg gagccccatg gtccggaagg agctggtggt ggctctgagt
     3841 catcttgtgg ttcagtatga aagcaatttc tgcaccgtgg ccctgcagtt catagaagag
     3901 gaaaagaact acgccttgcc ttctccagca accacagagg gagggagttt gaccccagtg
     3961 cgagacagcc cgtgcacccc cagacttcgt tctgtgagct cctatggaaa catccgtgct
     4021 gtcgccacag ccaggagcct caacaaatct ttgcagaacc tgagtttgac agaggaatct
     4081 ggtggcgcgg tggcgttctc ccccggaaac ctcagcacca gcagcagcgc cagcagcacc
     4141 ctgggcagcc ccgagaatga ggagcatatc ctgtccttcg agaccatcga caagatgcgc
     4201 cgcgccagct cctactcctc cctcaactcc ctcatcggag tttcctttaa cagtgtttac
     4261 actcagattt ggagagtcct gctgcacctg gctgctgacc cctatccaga ggtctcggac
     4321 gtggccatga aagtactcaa cagcatcgcc tacagggcca ccgtgaacgc ccggccgcag
     4381 cgcgtcctgg acacctcctc cctcacgcag tcggcccccg ccagccccac caacaagggc
     4441 gtgcacatcc accaggcggg gggctcccct ccggcgtcca gcaccagcag ctccagcctg
     4501 accaacgatg tggccaagca gccggtcagc cgagacttgc cttctggccg gccgggcacc
     4561 acaggccccg ctggggcgca gtacacccct cactcccacc agttcccccg gacacggaag
     4621 atgttcgaca agggcccaga gcagactgcg gacgacgcgg acgatgctgc tggacacaaa
     4681 agtttcatct ccgccacggt gcagacgggg ttctgcgact ggagcgcccg ctattttgcc
     4741 cagcccgtca tgaagatccc agaagagcac gacctggaga gtcagatccg caaggagcgg
     4801 gagtggcggt tcctgcgaaa cagccgtgtc aggaggcagg cccagcaagt cattcagaag
     4861 ggcattacga gattggacga ccaaatattt ctgaacagga accccggcgt cccctctgtg
     4921 gtgaaattcc accccttcac gccgtgcatc gctgtagccg acaaggacag catctgcttt
     4981 tgggactggg agaaagggga gaagctggat tatttccaca atgggaaccc tcggtacacg
     5041 agggtcactg ccatggagta tctgaatggc caggactgct cgcttctgct gacggccaca
     5101 gacgatggtg ccatcagggt ctggaagaat tttgctgatt tggaaaagaa cccagagatg
     5161 gtgaccgcgt ggcaggggct ctcggacatg ctgccaacga cgcgaggagc tgggatggtg
     5221 gtggactggg agcaggagac cggcctcctc atgagctcag gagacgtgcg gatcgtccgg
     5281 atctgggaca cagaccgtga gatgaaggtg caggacatcc ctacgggcgc agacagctgt
     5341 gtgacgagtc tgtcctgtga ttcccaccgc tcactcatcg tggctggcct cggtgacggc
     5401 tccatccgcg tctacgacag aaggatggca ctcagcgaat gccgcgtcat gacgtaccgg
     5461 gagcacacag cctgggtggt gaaggcctcc ctgcagaagc gtcccgacgg ccacatcgtg
     5521 agtgtgagcg tcaatggaga tgtgcgcatc tttgatcccc ggatgcctga gtcggtaaat
     5581 gtgcttcaga tcgtgaaggg gctgacggcc ctggacatcc acccccaggc ggacctgatc
     5641 gcatgtggct ccgtcaatca gttcaccgcc atctacaaca gcagcggaga gctcatcaac
     5701 aacatcaagt actacgacgg cttcatgggc cagcgggtcg gcgccatcag ctgcctggcc
     5761 ttccacccgc actggcctca cctggccgtg ggaagcaacg actactacat ctccgtgtac
     5821 tcggtggaga agcgtgtcag atagcggcgt gacccgggcc caccaggcca cggccgcctg
     5881 ctgtacatag tgaagctgtc actcgccggg gcacggggcg tcggctgctg cggccccgca
     5941 gtgtgaacgt tggctgctgc cttagctgct gatgacggca ggagggccct gctactcgct
     6001 tttgtctgtc ttcgctgtcg tgtctggaat gtcagggaag gggagggctc gggttgacgg
     6061 tggcttccca ctgagcacca gcatccaggt gcacccccgc ggccacggcg cctctgtccc
     6121 tctcctgttc tgtgtttctc tgagacgctg aaaggggaaa cacctcactt tatttccatg
     6181 taatcagagc attagctgca gaaaaacccc cagacagagc cctggcggag aggcaggcgc
     6241 tggggctcct acgggtccct ggggcagctg tccccatcag gccaagagcg agcgagaggc
     6301 gctgccccag ccaggcccac cacctctcac agtcagtgca cgcaagcagg gacatttcct
     6361 agccagctgg gggacactgg aaattcggga aaccaagaga gaggaagaag gagacgcccc
     6421 tccaactggc gggtgtgaag gaagccgccc aggggtccgg gctgtccttg gccgctggca
     6481 gcatcactga gcaggaagcg cacagcccac cctccccgca cctccaggtc tctggactcc
     6541 agttttggcc cctctcacac agagctgtca gcaggggccg ctgtggcggt gcacagggga
     6601 ggcaggtcct tggcgaggta gcccctgcct taatccacgg ggctcctttc cctccgaagg
     6661 gctgctcttc cccacaggcg cggggacagc agcccgacct gtggtctcca tgcctgtgcc
     6721 ctcacacagg tgtagcacac gcatgtgcag atggcaccac ggccggcacc tgggggcaca
     6781 cacatgcagg cggcgtggtc tccctgctct gtccccacac gttcctcaca tacaggcaag
     6841 aggcactgcc gggtcccgga cggctccggg tgacaccagc cccgtctcca gccttgagcc
     6901 gcccatgctg atgcgacctc ggctgacagc tgggcctgtg gtgcagacag gagctgtgtg
     6961 gacagtcccg cccaggaggg gccgcagggc gtgtatgagc agttttgcaa acagaacaca
     7021 accacaatga tggtattttg aaaagtgttc tttccgtgtt cgtcgggaat caggattatt
     7081 gagaggtgaa ggagccaggt ggcttcattc tggcggtgag aggcccatga ccacgggagt
     7141 gagagctggt gtggcgaggc ccggctctcc tgcggtgtgg ctggtggcct gccgtggcca
     7201 agagcatctt ctgggtggat ggaaccctgc ctggtcacat ttggccagag acacacctgg
     7261 ccctcagggg gctgagctgg agactgagct ggggctggcc gggacgtgac aaggcaggac
     7321 agaggcggcc cctccgctgc tcctttttgg aatgcgagct cccaccagaa gaaggttccg
     7381 gcacgaatcc catccccacg tctgggccga gaaagcagcc cgggtccgga aggtgtagag
     7441 agtcccggcc tcactcagct cacagggcgt gccaggcggc aacaccagaa tcttccagaa
     7501 gcccagctcc acccgcacac gcagcttccc atccagtcct tcaactcaat tcttacccaa
     7561 cacgcgtttc cgtttgtttt gagacaaaat caccacctgt caaaaggcag gtggctccag
     7621 aggggtcaag accccccccc ccgcccccgc tccaccctgg agcccacccc catgggcact
     7681 gcgtgccgcc tgcacgtggg ctgtcttcac aggtctgatg tgaaaattca atcacgacgt
     7741 taaccggctc gagagagcgc cggcctagag gctcattatc tatttatttt accaaacgcg
     7801 aattgagacg gactttgaca aaacacgaaa tggtaatgtg aagctaagag cagagagtga
     7861 ccaacagtaa acaacacgcg cagactcaaa aaaaaaaaaa aaagggcggc cgctaagtaa
     7921 gtaaggatcc ccagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta
     7981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg
     8101 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     8161 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8341 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8401 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8461 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctgtt
     8521 aacagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8581 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8641 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     8701 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     8761 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8821 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8881 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8941 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     9001 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     9061 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     9121 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     9181 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     9241 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     9301 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     9361 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     9421 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     9481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9841 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10741 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11281 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11341 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11401 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    11461 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11521 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    11581 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt ac
//
