LOCUS       sequence-413148-        2973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express CD44 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Du Y, Bradshaw WJ, Leisner TM, Annor-Gyamfi JK, Qian K, Bashore FM, 
            Sikdar A, Nwogbo FO, Ivanov AA, Frye SV, Gileadi O, Brennan PE, 
            Levey AI, Axtman AD, Pearce KH, Fu H, Katis VL
  TITLE     Discovery of FERM domain protein-protein interaction inhibitors for 
            MSN and CD44 as a potential therapeutic approach for Alzheimer's 
            disease.
  JOURNAL   J Biol Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382.
  PUBMED    37866628
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382."
COMMENT     Sequence Label: pFUW-VenusFlag-hCD44
FEATURES             Location/Qualifiers
     source          1..2973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             757..780
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2924..2948)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga
      721 gggggtggat ctggaggggg tggatctaag cttatggact acaaggacga cgatgacaag
      781 ggtggtggat ccgaattatc aacaagtttg tacaaaaaag ttggcatgga caagttttgg
      841 tggcacgcag cctggggact ctgcctcgtg ccgctgagcc tggcgcagat cgatttgaat
      901 ataacctgcc gctttgcagg tgtattccac gtggagaaaa atggtcgcta cagcatctct
      961 cggacggagg ccgctgacct ctgcaaggct ttcaatagca ccttgcccac aatggcccag
     1021 atggagaaag ctctgagcat cggatttgag acctgcaggt atgggttcat agaagggcat
     1081 gtggtgattc cccggatcca ccccaactcc atctgtgcag caaacaacac aggggtgtac
     1141 atcctcacat ccaacacctc ccagtatgac acatattgct tcaatgcttc agctccacct
     1201 gaagaagatt gtacatcagt cacagacctg cccaatgcct ttgatggacc aattaccata
     1261 actattgtta accgtgatgg cacccgctat gtccagaaag gagaatacag aacgaatcct
     1321 gaagacatct accccagcaa ccctactgat gatgacgtga gcagcggctc ctccagtgaa
     1381 aggagcagca cttcaggagg ttacatcttt tacacctttt ctactgtaca ccccatccca
     1441 gacgaagaca gtccctggat caccgacagc acagacagaa tccctgctac cagtacgtct
     1501 tcaaatacca tctcagcagg ctgggagcca aatgaagaaa atgaagatga aagagacaga
     1561 cacctcagtt tttctggatc aggcattgat gatgatgaag attttatctc cagcaccatt
     1621 tcaaccacac cacgggcttt tgaccacaca aaacagaacc aggactggac ccagtggaac
     1681 ccaagccatt caaatccgga agtgctactt cagacaacca caaggatgac tgatgtagac
     1741 agaaatggca ccactgctta tgaaggaaac tggaacccag aagcacaccc tcccctcatt
     1801 caccatgagc atcatgagga agaagagacc ccacattcta caagcacaat ccaggcaact
     1861 cctagtagta caacggaaga aacagctacc cagaaggaac agtggtttgg caacagatgg
     1921 catgagggat atcgccaaac acccagagaa gactcccatt cgacaacagg gacagctgca
     1981 gcctcagctc ataccagcca tccaatgcaa ggaaggacaa caccaagccc agaggacagt
     2041 tcctggactg atttcttcaa cccaatctca caccccatgg gacgaggtca tcaagcagga
     2101 agaaggatgg atatggactc cagtcatagt acaacgcttc agcctactgc aaatccaaac
     2161 acaggtttgg tggaagattt ggacaggaca ggacctcttt caatgacaac gcagcagagt
     2221 aattctcaga gcttctctac atcacatgaa ggcttggaag aagataaaga ccatccaaca
     2281 acttctactc tgacatcaag caataggaat gatgtcacag gtggaagaag agacccaaat
     2341 cattctgaag gctcaactac tttactggaa ggttatacct ctcattaccc acacacgaag
     2401 gaaagcagga ccttcatccc agtgacctca gctaagactg ggtcctttgg agttactgca
     2461 gttactgttg gagattccaa ctctaatgtc aatcgttcct tatcaggaga ccaagacaca
     2521 ttccacccca gtggggggtc ccataccact catggatctg aatcagatgg acactcacat
     2581 gggagtcaag aaggtggagc aaacacaacc tctggtccta taaggacacc ccaaattcca
     2641 gaatggctga tcatcttggc atccctcttg gccttggctt tgattcttgc agtttgcatt
     2701 gcagtcaaca gtcgaagaag gtgtgggcag aagaaaaagc tagtgatcaa cagtggcaat
     2761 ggagctgtgg aggacagaaa gccaagtgga ctcaacggag aggccagcaa gtctcaggaa
     2821 atggtgcatt tggtgaacaa ggagtcgtca gaaactccag accagtttat gacagctgat
     2881 gagacaagga acctgcagaa tgtggacatg aagattgggg tgtacccaac tttcttgtac
     2941 aaagtggttg atctagaggg ccctattcta tag
//
