LOCUS       sequence-413152-       10185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B1 mutant E234Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pMx/ATP8B1(E234Q)-HA
FEATURES             Location/Qualifiers
     source          1..10185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4096..4107
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(4461..4472)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5761..5785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5817..5843
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6012..6032)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6017..6346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6197..6332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6703..7497
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6757..6776)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7367..7386
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7690..8159
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8289..8306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8460..9048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8540..8559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9212..10003,10004..10072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9835..9854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10073..10177)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ctctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccatgagt acagaaagag actcagaaac gacgtttgac gaggattctc
     2041 agcctaatga cgaagtggtt ccctacagtg atgatgaaac agaagatgaa cttgatgacc
     2101 aggggtctgc tgttgaacca gaacaaaacc gagtcaacag ggaagcagag gagaaccggg
     2161 agccattcag aaaagaatgt acatggcaag tcaaagcaaa cgatcgcaag taccacgaac
     2221 aacctcactt tatgaacaca aaattcttgt gtattaagga gagtaaatat gcgaataatg
     2281 caattaaaac atacaagtac aacgcattta cctttatacc aatgaatctg tttgagcagt
     2341 ttaagagagc agccaattta tatttcctgg ctcttcttat cttacaggca gttcctcaaa
     2401 tctctaccct ggcttggtac accacactag tgcccctgct tgtggtgctg ggcgtcactg
     2461 caatcaaaga cctggtggac gatgtggctc gccataaaat ggataaggaa atcaacaata
     2521 ggacgtgtga agtcattaag gatggcaggt tcaaagttgc taagtggaaa gaaattcaag
     2581 ttggagacgt cattcgtctg aaaaaaaatg actttgttcc agctgacatt ctcctgctgt
     2641 ctagctctga gcctaacagc ctctgctatg tggaaacagc agaactggac ggacaaacca
     2701 atttaaaatt taagatgtca cttgaaatca cagaccagta cctccaaaga gaagatacat
     2761 tggctacatt tgatggtttt attgaatgtg aagaacccaa taaccgactg gataagttta
     2821 caggaacact attttggaga aacacaagtt ttcctttgga tgctgataaa attttgttac
     2881 gtggctgtgt aattaggaac accgatttct gccacggctt agtcattttt gcaggtgctg
     2941 acactaaaat aatgaagaat agtgggaaaa ccagatttaa aagaactaaa attgattact
     3001 tgatgaacta catggtttac acgatctttg ttgttcttat tctgctttct gctggtcttg
     3061 ccatcggcca tgcttattgg gaagcacagg tgggcaattc ctcttggtac ctctatgatg
     3121 gagaagacga tacaccctcc taccgtggat tcctcatttt ctggggctat atcattgttc
     3181 tcaacaccat ggtacccatc tctctctatg tcagcgtgga agtgattcgt cttggacaga
     3241 gtcacttcat caactgggac ctgcaaatgt actatgctga gaaggacaca cccgcaaaag
     3301 ctagaaccac cacactcaat gaacagctcg ggcagatcca ttatatcttc tctgataaga
     3361 cggggacact cacacaaaat atcatgacct ttaaaaagtg ctgtatcaac gggcagatat
     3421 atggggacca tcgggatgcc tctcaacaca accacaacaa aatagagcaa gttgatttta
     3481 gctggaatac atatgctgat gggaagcttg cattttatga ccactatctt attgagcaaa
     3541 tccagtcagg gaaagagcca gaagtacgac agttcttctt cttgctcgca gtttgccaca
     3601 cagtcatggt ggataggact gatggtcagc tcaactacca ggcagcctct cccgatgaag
     3661 gtgccctggt aaacgctgcc aggaactttg gctttgcctt cctcgccagg acccagaaca
     3721 ccatcaccat cagtgaactg ggcactgaaa ggacttacaa tgttcttgcc attttggact
     3781 tcaacagtga ccggaagcga atgtctatca ttgtaagaac cccagaaggc aatatcaagc
     3841 tttactgtaa aggtgctgac actgttattt atgaacggtt acatcgaatg aatcctacta
     3901 agcaagaaac acaggatgcc ctggatatct ttgcaaatga aactcttaga accctatgcc
     3961 tttgctacaa ggaaattgaa gaaaaagaat ttacagaatg gaataaaaag tttatggctg
     4021 ccagtgtggc ctccaccaac cgggacgaag ctctggataa agtatatgag gagattgaaa
     4081 aagacttaat tctcctggga gctacagcta ttgaagacaa gctacaggat ggagttccag
     4141 aaaccatttc aaaacttgca aaagctgaca ttaagatctg ggtgcttact ggagacaaaa
     4201 aggaaactgc tgaaaatata ggatttgctt gtgaacttct gactgaagac accaccatct
     4261 gctatgggga ggatattaat tctcttcttc atgcaaggat ggaaaaccag aggaatagag
     4321 gtggcgtcta cgcaaagttt gcacctcctg tgcaggaatc tttttttcca cccggtggaa
     4381 accgtgcctt aatcatcact ggttcttggt tgaatgaaat tcttctcgag aaaaagacca
     4441 agagaaataa gattctgaag ctgaagttcc caagaacaga agaagaaaga cggatgcgga
     4501 cccaaagtaa aaggaggcta gaagctaaga aagagcagcg gcagaaaaac tttgtggacc
     4561 tggcctgcga gtgcagcgca gtcatctgct gccgcgtcac ccccaagcag aaggccatgg
     4621 tggtggacct ggtgaagagg tacaagaaag ccatcacgct ggccatcgga gatggggcca
     4681 atgacgtgaa catgatcaaa actgcccaca ttggcgttgg aataagtgga caagaaggaa
     4741 tgcaagctgt catgtcgagt gactattcct ttgctcagtt ccgatatctg cagaggctac
     4801 tgctggtgca tggccgatgg tcttacataa ggatgtgcaa gttcctacga tacttctttt
     4861 acaaaaactt tgcctttact ttggttcatt tctggtactc cttcttcaat ggctactctg
     4921 cgcagactgc atacgaggat tggttcatca ccctctacaa cgtgctgtac accagcctgc
     4981 ccgtgctcct catggggctg ctcgaccagg atgtgagtga caaactgagc ctccgattcc
     5041 ctgggttata catagtggga caaagagact tactattcaa ctataagaga ttctttgtaa
     5101 gcttgttgca tggggtccta acatcgatga tcctcttctt catacctctt ggagcttatc
     5161 tgcaaaccgt agggcaggat ggagaggcac cttccgacta ccagtctttt gccgtcacca
     5221 ttgcctctgc tcttgtaata acagtcaatt tccagattgg cttggatact tcttattgga
     5281 cttttgtgaa tgctttttca atttttggaa gcattgcact ttattttggc atcatgtttg
     5341 actttcatag tgctggaata catgttctct ttccatctgc atttcaattt acaggcacag
     5401 cttcaaacgc tctgagacag ccatacattt ggttaactat catcctgact gttgctgtgt
     5461 gcttactacc cgtcgttgcc attcgattcc tgtcaatgac catctggcca tcagaaagtg
     5521 ataagatcca gaagcatcgc aagcggttga aggcggagga gcagtggcag cgacggcagc
     5581 aggtgttccg ccggggcgtg tcaacgcggc gctcggccta cgccttctcg caccagcggg
     5641 gctacgcgga cctcatctcc tccgggcgca gcatccgcaa gaagcgctcg ccgcttgatg
     5701 ccatcgtggc ggatggcacc gcggagtaca ggcgcaccgg ggactcgtcg agatatctag
     5761 acccagcttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa ctcgagtatc
     5821 catacgatgt accagactac gcagagatcc actagttcaa ttccagctga gcgccggtcg
     5881 ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc
     5941 cgtatttcgc gtaaggaaat ccattatgta ctatttaaac tcgagcggcc gccagcacag
     6001 tggtcgactg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6301 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttcacgctgc
     6361 cgcaagcact cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg
     6421 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
     6481 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
     6541 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     6601 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     6661 ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6721 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6781 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6841 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6901 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6961 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     7021 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     7081 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     7141 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     7201 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     7261 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     7321 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7381 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7441 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     7501 ggactctggg gttcgcatcg tcgacgataa aataaaagat tttatttagt ctccagaaaa
     7561 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     7621 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     7681 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     7741 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     7801 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     7861 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     7921 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     7981 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     8041 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     8101 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     8161 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     8221 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8281 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8341 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8401 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8461 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8521 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8581 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8641 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8701 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8761 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8821 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8881 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8941 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9001 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9061 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9121 atgagattat caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct
     9181 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9241 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9301 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9361 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9421 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9481 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9541 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9601 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9661 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9721 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9781 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9841 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9901 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9961 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10021 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10081 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10141 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttc
//
