LOCUS       sequence-413153-        4845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Un1Cas12f1 Gateway entry plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Tang X, Eid A, Zhang R, Cheng Y, Liu A, Chen Y, Chen P, Zhang Y, Qi 
            Y
  TITLE     Genome editing in rice and tomato with a small Un1Cas12f1 nuclease.
  JOURNAL   Plant Genome. 2024 May 28:e20465. doi: 10.1002/tpg2.20465.
  PUBMED    38807445
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2024 May 28:e20465. doi: 10.1002/tpg2.20465."
COMMENT     Sequence Label: pYPQ-Un1Cas12f1
FEATURES             Location/Qualifiers
     source          1..4845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2585..2605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4217..4264
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4295..4547
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4592..4715
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4732..4751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4733..4751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4756..4772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4756..4772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggctccgaag aagaagagga aggttggcat ccacggggtg ccagctgcta tggccaagaa
     2641 cactataact aagacgttaa aactacggat tgttcgtcct tataactccg ctgaagtcga
     2701 gaagatcgtc gctgatgaaa agaacaacag ggagaagata gctttggaaa agaataagga
     2761 taaggtaaaa gaggcgtgtt cgaagcatct gaaggtggcg gcctactgca cgacccaggt
     2821 ggagaggaat gcatgtctgt tctgcaaggc caggaagctc gatgacaaat tctaccagaa
     2881 gctacgcggc cagttccccg acgctgtgtt ctggcaggag atttctgaaa ttttccgcca
     2941 actgcagaag caagcagcgg agatctacaa ccagagcttg atcgagctgt actacgaaat
     3001 attcattaag ggcaagggta tcgcaaacgc ctcctcagtt gagcactacc tcagcgacgt
     3061 ctgctatacc agggccgcgg agcttttcaa aaatgcagca atagcatccg gcctccgctc
     3121 caagatcaag agtaatttca ggctcaagga gctgaagaac atgaaatcag ggctgccgac
     3181 caccaaatct gacaactttc ctataccgct agtgaagcag aaaggggggc agtacaccgg
     3241 ctttgaaatt agcaaccaca actctgattt tatcatcaag attccatttg gaagatggca
     3301 agttaagaag gaaattgaca agtatagacc ttgggagaag tttgatttcg agcaggttca
     3361 aaagagcccc aagccaatat cactcctcct ctcgacccaa cggcgcaaga ggaacaaggg
     3421 gtggtctaag gacgagggga cagaagcaga aattaaaaaa gtgatgaatg gtgattatca
     3481 aacatcttac atcgaggtga aaagaggaag caagataggc gagaaaagtg cgtggatgct
     3541 gaacctttcg attgatgtac ctaaaattga taaaggagtt gatccttcaa tcattggcgg
     3601 catcgacgtg ggtgtcaagt cgccgctcgt gtgcgccatc aacaacgcgt tctccaggta
     3661 ttcaatctcc gacaatgatt tgttccattt taataaaaag atgtttgcac gacgccgtat
     3721 cttgcttaag aaaaatcgcc acaagcgcgc cggccacgga gctaagaata agctcaagcc
     3781 catcacaata ttaactgaaa aatcagagcg gttccggaag aagctcatcg aacggtgggc
     3841 gtgcgagatc gccgacttct tcatcaaaaa caaggtcggc acagtgcaga tggagaattt
     3901 ggagagcatg aagaggaaag aagattcata cttcaacatc aggctcagag gtttttggcc
     3961 gtacgccgag atgcagaata aaattgaatt taagctgaaa caatatggta ttgagataag
     4021 aaaggttgct ccaaataata caagtaaaac ctgctctaag tgtggacatt tgaacaatta
     4081 tttcaatttt gagtatagga agaagaacaa gttcccacac ttcaagtgtg agaaatgcaa
     4141 ctttaaggaa aatgctgact acaatgccgc cctgaatatt tctaacccaa aactaaaaag
     4201 tactaaggag gagccgaagc ggccagcggc gacgaagaag gcggggcagg cgaagaagaa
     4261 gaagtgagac gactagtggc ggccgccgac gtccgatcgt tcaaacattt ggcaataaag
     4321 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     4381 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     4441 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     4501 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattgatccc
     4561 ccctcgacag cttccggaaa gggcgaattc gcaactttgt atacaaaagt tgaacgagaa
     4621 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     4681 taatactgta aaacacaaca tatccagtca ctatgccatc cagctgatat cccctatagt
     4741 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     4801 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctc
//
