LOCUS       sequence-413172-        4415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-NL63-Envelope
FEATURES             Location/Qualifiers
     source          1..4415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(128..139)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(388..487)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(504..523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(505..523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(528..544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(528..544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             975..1766
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1859..2447
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2348..2367
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2601..2618
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2777..2804)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4234..4320)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4369..4391
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4383..4400
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4384..4400
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct tccttcgatt aattgatgac aatggtattg tcctcaattc cattttatgg
      181 ctccttgtta tgatattttt ctttgtgttg gcaatgacct ttattaaact gattcaattg
      241 tgttttactt gtcattattt ttttagtagg acattatatc aaccagttta taaaattttt
      301 cttgcttacc aagattatat gcaaatagca cctgttccag ctgaagtact aaatgtcctt
      361 aattaagacg cgatcgcggc ctcatgggcc cagctttctt gtacaaagtt ggcattataa
      421 aaaataattg ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
      481 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
      541 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
      601 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
      661 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
      721 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      781 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
      841 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
      901 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
      961 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1021 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1081 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     1141 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1201 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1261 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1321 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1381 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1441 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1501 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     1561 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1621 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1681 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     1741 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     1801 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1981 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2521 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2581 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2641 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2701 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     2761 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     2821 ggcactgatg aatcccctaa tgattttggt aaaaatcatt aagttaaggt ggatacacat
     2881 cttgtcatat gatcaaatgg tttcgcgaaa aatcaataat cagacaacaa gatgtgcgaa
     2941 ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc
     3001 aacagcttaa cgttggcttg ccacgcatta cttgactgta aaactctcac tcttaccgaa
     3061 cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga
     3121 ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc
     3181 tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga tattcgtgag
     3241 caaaaacgac ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt
     3301 tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta
     3361 gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc
     3421 tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta
     3481 agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta
     3541 catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat
     3601 ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc
     3661 tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag
     3721 ccatgggttc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat
     3781 atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac
     3841 ggactaggcc tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta
     3901 atcgccctga tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt
     3961 tgggacaagc acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc
     4021 ttaggcatgg aagttttgcg gcattctggc tacacaataa caagggaaga cttactcgtg
     4081 gctgcaaccc tactagctca aaatttattc acacatggtt acgctttggg gaaattatga
     4141 ggggatctct cagtgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     4201 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     4261 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     4321 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     4381 gttgtaaaac gacggccagt cttaagctcg ggccc
//
