LOCUS       sequence-413205-        2610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human MSN in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Du Y, Bradshaw WJ, Leisner TM, Annor-Gyamfi JK, Qian K, Bashore FM, 
            Sikdar A, Nwogbo FO, Ivanov AA, Frye SV, Gileadi O, Brennan PE, 
            Levey AI, Axtman AD, Pearce KH, Fu H, Katis VL
  TITLE     Discovery of FERM domain protein-protein interaction inhibitors for 
            MSN and CD44 as a potential therapeutic approach for Alzheimer's 
            disease.
  JOURNAL   J Biol Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382.
  PUBMED    37866628
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382."
COMMENT     Sequence Label: pDEST27-GST-hMSN
FEATURES             Location/Qualifiers
     source          1..2610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             113..766
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     724..746
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     protein_bind    794..818
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2559..2583)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
       61 agaagacacc gggaccgatc cagcctccgg actctagcct aggccgcgga ccatggcccc
      121 tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc ttttggaata
      181 tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa
      241 caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt
      301 taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg
      361 tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag
      421 atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct
      481 tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt
      541 aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt
      601 atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat
      661 tgaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt
      721 gcagggctgg caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg atctggttcc
      781 gcgttctaga tcaacaagtt tgtacaaaaa agcaggctcc accatgccca aaacgatcag
      841 tgtgcgtgtg accaccatgg atgcagagct ggagtttgcc atccagccca acaccaccgg
      901 gaagcagcta tttgaccagg tggtgaaaac tattggcttg agggaagttt ggttctttgg
      961 tctgcagtac caggacacta aaggtttctc cacctggctg aaactcaata agaaggtgac
     1021 tgcccaggat gtgcggaagg aaagccccct gctctttaag ttccgtgcca agttctaccc
     1081 tgaggatgtg tccgaggaat tgattcagga catcactcag cgcctgttct ttctgcaagt
     1141 gaaagagggc attctcaatg atgatattta ctgcccgcct gagaccgctg tgctgctggc
     1201 ctcgtatgct gtccagtcta agtatggcga cttcaataag gaagtgcata agtctggcta
     1261 cctggccgga gacaagttgc tcccgcagag agtcctggaa cagcacaaac tcaacaagga
     1321 ccagtgggag gagcggatcc aggtgtggca tgaggaacac cgtggcatgc tcagggagga
     1381 tgctgtcctg gaatatctga agattgctca agatctggag atgtatggtg tgaactactt
     1441 cagcatcaag aacaagaaag gctcagagct gtggctgggg gtggatgccc tgggtctcaa
     1501 catctatgag cagaatgaca gactaactcc caagataggc ttcccctgga gtgaaatcag
     1561 gaacatctct ttcaatgata agaaatttgt catcaagccc attgacaaaa aagccccgga
     1621 cttcgtcttc tatgctcccc ggctgcggat taacaagcgg atcttggcct tgtgcatggg
     1681 gaaccatgaa ctatacatgc gccgtcgcaa gcctgatacc attgaggtgc agcagatgaa
     1741 ggcacaggcc cgggaggaga agcaccagaa gcagatggag cgtgctatgc tggaaaatga
     1801 gaagaagaag cgtgaaatgg cagagaagga gaaagagaag attgaacggg agaaggagga
     1861 gctgatggag aggctgaagc agatcgagga acagactaag aaggctcagc aagaactgga
     1921 agaacagacc cgtagggctc tggaacttga gcaggaacgg aagcgtgccc agagcgaggc
     1981 tgaaaagctg gccaaggagc gtcaagaagc tgaagaggcc aaggaggcct tgctgcaggc
     2041 ctcccgggac cagaaaaaga ctcaggaaca gctggccttg gaaatggcag agctgacagc
     2101 tcgaatctcc cagctggaga tggcccgaca gaagaaggag agtgaggctg tggagtggca
     2161 gcagaaggcc cagatggtac aggaagactt ggagaagacc cgtgctgagc tgaagactgc
     2221 catgagtaca cctcatgtgg cagagcctgc tgagaatgag caggatgagc aggatgagaa
     2281 tggggcagag gctagtgctg acctacgggc tgatgctatg gccaaggacc gcagtgagga
     2341 ggaacgtacc actgaggcag agaagaatga gcgtgtgcag aagcacctga aggccctcac
     2401 ttcggagctg gccaatgcca gagatgagtc caagaagact gccaatgaca tgatccatgc
     2461 tgagaacatg cgactgggcc gagacaaata caagaccctg cgccagatcc ggcagggcaa
     2521 caccaagcag cgcattgacg aatttgagtc tatgttggac ccagctttct tgtacaaagt
     2581 ggttgatcgc gtgcatgcga cgtcatagct
//
