LOCUS       sequence-413213-        9382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Cloning Vector for CRISPRoffv2.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pENTR1a-CRISPRoffv2.1
FEATURES             Location/Qualifiers
     source          1..9382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2416..2442,2443..2445,2446..4932,4933..4935,
                     4936..6519)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6523..6549
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6529..6555
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             6568..6588
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6595..6615
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6655..7350
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             7396..7581
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     protein_bind    complement(7611..7710)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7822..7841)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7833..8642
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7900..7919)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8732..9320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9221..9240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
      541 accatgaacc atgaccagga atttgacccc ccaaaggttt acccacctgt gccagctgag
      601 aagaggaagc ccatccgcgt gctgtctctc tttgatggga ttgctacagg gctcctggtg
      661 ctgaaggacc tgggcatcca agtggaccgc tacattgcct ccgaggtgtg tgaggactcc
      721 atcacggtgg gcatggtgcg gcaccaggga aagatcatgt acgtcgggga cgtccgcagc
      781 gtcacacaga agcatatcca ggagtggggc ccattcgacc tggtgattgg aggcagtccc
      841 tgcaatgacc tctccattgt caaccctgcc cgcaagggac tttatgaggg tactggccgc
      901 ctcttctttg agttctaccg cctcctgcat gatgcgcggc ccaaggaggg agatgatcgc
      961 cccttcttct ggctctttga gaatgtggtg gccatgggcg ttagtgacaa gagggacatc
     1021 tcgcgatttc ttgagtctaa ccccgtgatg attgacgcca aagaagtgtc tgctgcacac
     1081 agggcccgtt acttctgggg taaccttcct ggcatgaaca ggcctttggc atccactgtg
     1141 aatgataagc tggagctgca agagtgtctg gagcacggca gaatagccaa gttcagcaaa
     1201 gtgaggacca ttaccaccag gtcaaactct ataaagcagg gcaaagacca gcatttcccc
     1261 gtcttcatga acgagaagga ggacatcctg tggtgcactg aaatggaaag ggtgtttggc
     1321 ttccccgtcc actacacaga cgtctccaac atgagccgct tggcgaggca gagactgctg
     1381 ggccgatcgt ggagcgtgcc ggtcatccgc cacctcttcg ctccgctgaa ggaatatttt
     1441 gcttgtgtgt ctagcggcaa tagtaacgct aacagccgcg ggccgagctt cagcagcggc
     1501 ctggtgccgt taagcttgcg cggcagccat atgggcccta tggagatata caagacagtg
     1561 tctgcatgga agagacagcc agtgcgggta ctgagcctct tcagaaacat cgacaaggta
     1621 ctaaagagtt tgggcttctt ggaaagcggt tctggttctg ggggaggaac gctgaagtac
     1681 gtggaagatg tcacaaatgt cgtgaggaga gacgtggaga aatggggccc ctttgacctg
     1741 gtgtacggct cgacgcagcc cctaggcagc tcttgtgatc gctgtcccgg ctggtacatg
     1801 ttccagttcc accggatcct gcagtatgcg ctgcctcgcc aggagagtca gcggcccttc
     1861 ttctggatat tcatggacaa tctgctgctg actgaggatg accaagagac aactacccgc
     1921 ttccttcaga cagaggctgt gaccctccag gatgtccgtg gcagagacta ccagaatgct
     1981 atgcgggtgt ggagcaacat tccagggctg aagagcaagc atgcgcccct gaccccaaag
     2041 gaagaagagt atctgcaagc ccaagtcaga agcaggagca agctggacgc cccgaaagtt
     2101 gacctcctgg tgaagaactg ccttctcccg ctgagagagt acttcaagta tttttctcaa
     2161 aactcacttc ctcttggagg gccgagctct ggcgcacccc caccaagtgg agggtctcct
     2221 gccgggtccc caacatctac tgaagaaggc accagcgaat ccgcaacgcc cgagtcaggc
     2281 cctggtacct ccacagaacc atctgaaggt agtgcgcctg gttccccagc tggaagccct
     2341 acttccaccg aagaaggcac gtcaaccgaa ccaagtgaag gatctgcccc tgggaccagc
     2401 actgaaccat ctgagatgga caagaagtat tctatcggac tggccatcgg gactaatagc
     2461 gtcgggtggg ccgtgatcac tgacgagtac aaggtgccct ctaagaagtt caaggtgctc
     2521 gggaacaccg accggcattc catcaagaaa aatctgatcg gagctctcct ctttgattca
     2581 ggggagaccg ctgaagcaac ccgcctcaag cggactgcta gacggcggta caccaggagg
     2641 aagaaccgga tttgttacct tcaagagata ttctccaacg aaatggcaaa ggtcgacgac
     2701 agcttcttcc ataggctgga agaatcattc ctcgtggaag aggataagaa gcatgaacgg
     2761 catcccatct tcggtaatat cgtcgacgag gtggcctatc acgagaaata cccaaccatc
     2821 taccatcttc gcaaaaagct ggtggactca accgacaagg cagacctccg gcttatctac
     2881 ctggccctgg cccacatgat caagttcaga ggccacttcc tgatcgaggg cgacctcaat
     2941 cctgacaata gcgatgtgga taaactgttc atccagctgg tgcagactta caaccagctc
     3001 tttgaagaga accccatcaa tgcaagcgga gtcgatgcca aggccattct gtcagcccgg
     3061 ctgtcaaaga gccgcagact tgagaatctt atcgctcagc tgccgggtga aaagaaaaat
     3121 ggactgttcg ggaacctgat tgctctttca cttgggctga ctcccaattt caagtctaat
     3181 ttcgacctgg cagaggatgc caagctgcaa ctgtccaagg acacctatga tgacgatctc
     3241 gacaacctcc tggcccagat cggtgaccaa tacgccgacc ttttccttgc tgctaagaat
     3301 ctttctgacg ccatcctgct gtctgacatt ctccgcgtga acactgaaat caccaaggcc
     3361 cctctttcag cttcaatgat taagcggtat gatgagcacc accaggacct gaccctgctt
     3421 aaggcactcg tccggcagca gcttccggag aagtacaagg aaatcttctt tgaccagtca
     3481 aagaatggat acgccggcta catcgacgga ggtgcctccc aagaggaatt ttataagttt
     3541 atcaaaccta tccttgagaa gatggacggc accgaagagc tcctcgtgaa actgaatcgg
     3601 gaggatctgc tgcggaagca gcgcactttc gacaatggga gcattcccca ccagatccat
     3661 cttggggagc ttcacgccat ccttcggcgc caagaggact tctacccctt tcttaaggac
     3721 aacagggaga agattgagaa aattctcact ttccgcatcc cctactacgt gggacccctc
     3781 gccagaggaa atagccggtt tgcttggatg accagaaagt cagaagaaac tatcactccc
     3841 tggaacttcg aagaggtggt ggacaaggga gccagcgctc agtcattcat cgaacggatg
     3901 actaacttcg ataagaacct ccccaatgag aaggtcctgc cgaaacattc cctgctctac
     3961 gagtacttta ccgtgtacaa cgagctgacc aaggtgaaat atgtcaccga agggatgagg
     4021 aagcccgcat tcctgtcagg cgaacaaaag aaggcaattg tggaccttct gttcaagacc
     4081 aatagaaagg tgaccgtgaa gcagctgaag gaggactatt tcaagaaaat tgaatgcttc
     4141 gactctgtgg agattagcgg ggtcgaagat cggttcaacg caagcctggg tacctaccat
     4201 gatctgctta agatcatcaa ggacaaggat tttctggaca atgaggagaa cgaggacatc
     4261 cttgaggaca ttgtcctgac tctcactctg ttcgaggacc gggaaatgat cgaggagagg
     4321 cttaagacct acgcccatct gttcgacgat aaagtgatga agcaacttaa acggagaaga
     4381 tataccggat ggggacgcct tagccgcaaa ctcatcaacg gaatccggga caaacagagc
     4441 ggaaagacca ttcttgattt ccttaagagc gacggattcg ctaatcgcaa cttcatgcaa
     4501 cttatccatg atgattccct gacctttaag gaggacatcc agaaggccca agtgtctgga
     4561 caaggtgact cactgcacga gcatatcgca aatctggctg gttcacccgc tattaagaag
     4621 ggtattctcc agaccgtgaa agtcgtggac gagctggtca aggtgatggg tcgccataaa
     4681 ccagagaaca ttgtcatcga gatggccagg gaaaaccaga ctacccagaa gggacagaag
     4741 aacagcaggg agcggatgaa aagaattgag gaagggatta aggagctcgg gtcacagatc
     4801 cttaaagagc acccggtgga aaacacccag cttcagaatg agaagctcta tctgtactac
     4861 cttcaaaatg gacgcgatat gtatgtggac caagagcttg atatcaacag gctctcagac
     4921 tacgacgtgg acgccatcgt ccctcagagc ttcctcaaag acgactcaat tgacaataag
     4981 gtgctgactc gctcagacaa gaaccgggga aagtcagata acgtgccctc agaggaagtc
     5041 gtgaaaaaga tgaagaacta ttggcgccag cttctgaacg caaagctgat cactcagcgg
     5101 aagttcgaca atctcactaa ggctgagagg ggcggactga gcgaactgga caaagcagga
     5161 ttcattaaac ggcaacttgt ggagactcgg cagattacta aacatgtcgc ccaaatcctt
     5221 gactcacgca tgaataccaa gtacgacgaa aacgacaaac ttatccgcga ggtgaaggtg
     5281 attaccctga agtccaagct ggtcagcgat ttcagaaagg actttcaatt ctacaaagtg
     5341 cgggagatca ataactatca tcatgctcat gacgcatatc tgaatgccgt ggtgggaacc
     5401 gccctgatca agaagtaccc aaagctggaa agcgagttcg tgtacggaga ctacaaggtc
     5461 tacgacgtgc gcaagatgat tgccaaatct gagcaggaga tcggaaaggc caccgcaaag
     5521 tacttcttct acagcaacat catgaatttc ttcaagaccg aaatcaccct tgcaaacggt
     5581 gagatccgga agaggccgct catcgagact aatggggaga ctggcgaaat cgtgtgggac
     5641 aagggcagag atttcgctac cgtgcgcaaa gtgctttcta tgcctcaagt gaacatcgtg
     5701 aagaaaaccg aggtgcaaac cggaggcttt tctaaggaat caatcctccc caagcgcaac
     5761 tccgacaagc tcattgcaag gaagaaggat tgggacccta agaagtacgg cggattcgat
     5821 tcaccaactg tggcttattc tgtcctggtc gtggctaagg tggaaaaagg aaagtctaag
     5881 aagctcaaga gcgtgaagga actgctgggt atcaccatta tggagcgcag ctccttcgag
     5941 aagaacccaa ttgactttct cgaagccaaa ggttacaagg aagtcaagaa ggaccttatc
     6001 atcaagctcc caaagtatag cctgttcgaa ctggagaatg ggcggaagcg gatgctcgcc
     6061 tccgctggcg aacttcagaa gggtaatgag ctggctctcc cctccaagta cgtgaatttc
     6121 ctctaccttg caagccatta cgagaagctg aaggggagcc ccgaggacaa cgagcaaaag
     6181 caactgtttg tggagcagca taagcattat ctggacgaga tcattgagca gatttccgag
     6241 ttttctaaac gcgtcattct cgctgatgcc aacctcgata aagtccttag cgcatacaat
     6301 aagcacagag acaaaccaat tcgggagcag gctgagaata tcatccacct gttcaccctc
     6361 accaatcttg gtgcccctgc cgcattcaag tacttcgaca ccaccatcga ccggaaacgc
     6421 tatacctcca ccaaagaagt gctggacgcc accctcatcc accagagcat caccggactt
     6481 tacgaaactc ggattgacct ctcacagctc ggaggggatg cctatcccta tgacgtgccc
     6541 gattatgcca gcctgggcag cggctccccc aagaaaaaac gcaaggtgga agatcctaag
     6601 aaaaagcgga aagtggacgg cattggtagt gggagcaacg gcagcagcgg atccagcgag
     6661 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
     6721 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     6781 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
     6841 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
     6901 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     6961 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     7021 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
     7081 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
     7141 ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc
     7201 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
     7261 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
     7321 ctccctagca aactggggca caagcttaat ggcggtggcg gagggatgga tgctaagtca
     7381 ctaactgcct ggtcccggac actggtgacc ttcaaggatg tatttgtgga cttcaccagg
     7441 gaggagtgga agctgctgga cactgctcag cagatcgtgt acagaaatgt gatgctggag
     7501 aactataaga acctggtttc cttgggttat cagcttacta agccagatgt gatcctccgg
     7561 ttggagaagg gagaagagcc ctagtaagcg gccgcactcg agatatctag acccagcttt
     7621 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     7681 cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc
     7741 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     7801 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     7861 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     7921 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     7981 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     8041 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     8101 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     8161 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgtccctg cgccggttgc
     8221 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     8281 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     8341 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     8401 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     8461 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     8521 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     8581 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     8641 aatcagaatt ggttaattgg ttgtaacatt attcagattg ggccccgttc cactgagcgt
     8701 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8761 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8821 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8881 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8941 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9001 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9061 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9121 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9181 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9241 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9301 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9361 gctggccttt tgctcacatg tt
//
