LOCUS       sequence-413214-       10376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-EgI-BlastR
FEATURES             Location/Qualifiers
     source          1..10376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2256..2272)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2257..2273)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        2380..3566
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2611..3557
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3514..3534
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3639..3763
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3788..3793,3794..3811,3812..3818)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3872..4531
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3938..3957)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4873..5178
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5057..5076
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5219..5343)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5488..5950
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5544..5561)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5771..5790
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5954..6352
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7585..7818
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7890..8011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7927..7946)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7981..8000
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8113..8132
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8397..8415)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8483..8587
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8588..8656,8657..9448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8806..8825)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9619..10207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10108..10127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgacct cgacggatcg
     2281 ggagatctcg accgagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2341 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2401 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     2461 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2521 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2581 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     2641 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     2701 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     2761 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     2821 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     2881 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     2941 ggccaagatc gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3001 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3061 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3121 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3181 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3241 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3301 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgatcgagct
     3361 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     3421 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     3481 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3541 tttttcttcc atttcaggtg tcgtgaggaa ttagcttgac caagcttggt accgagctcg
     3601 gatccactag taacggccgc cagtgtgctg gaattatcac aagtttgtac aaaaaagctg
     3661 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     3721 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
     3781 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
     3841 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     3901 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     3961 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     4021 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     4081 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     4141 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     4201 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     4261 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     4321 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     4381 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     4441 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     4501 gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag
     4561 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
     4621 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
     4681 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
     4741 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
     4801 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
     4861 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     4921 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
     4981 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     5041 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     5101 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     5161 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
     5221 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     5281 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     5341 ggtgataatt ctgcagatat ccatcacact ggcggccgct cgagcgcccc tctcccctaa
     5401 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5461 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5521 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5581 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5641 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5701 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5761 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5821 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5881 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5941 acgatgataa tccatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     6001 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     6061 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     6121 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     6181 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     6241 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     6301 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagctagcct
     6361 agtgccattt gttcagtggt tcgtagggct ttcccccact gtttggcttt cagttatatg
     6421 gatgatgtgg tattgggggc caagtctgta cagcatcttg agtccctttt taccgctgtt
     6481 accaattttc ttttgtcttt gggtatacat ttaaacccta acaaaacaaa gagatggggt
     6541 tactctctaa attttatggg ttatgtcatt ggatgttatg ggtccttgcc acaagaacac
     6601 atcatacaaa aaatcaaaga atgttttaga aaacttccta ttaacaggcc tattgattgg
     6661 aaagtatgtc aacgaattgt gggtcttttg ggttttgctg ccccttttac acaatgtggt
     6721 tatcctgcgt tgatgccttt gtatgcatgt attcaatcta agcaggcttt cactttctcg
     6781 ccaacttaca aggcctttct gtgtaaacaa tacctgaacc tttaccccgt tgcccggcaa
     6841 cggccaggtc tgtgccaagt gtttgctgac gcaaccccca ctggctgggg cttggtcatg
     6901 ggccatcagc gcatgcgtgg aaccttttcg gctcctctgc cgatccatac tgcggaactc
     6961 ctagccgctt gttttgctcg cagcaggtct ggagcaaaca ttatcgggac tgataactct
     7021 gttgtcctat cccgcaaata tacatcgttt ccatggctgc taggctgtgc tgccaactgg
     7081 atcctgcgcg ggacgtcctt tgtttacgtc ccgtcggcgc tgaatcctgc ggacgaccct
     7141 tctcggggtc gcttgggact ctctcgtccc cttctccgtc tgccgttccg accgaccacg
     7201 gggcgcacct ctctttacgc ggactccccg tctgtgcctt ctcatctgcc ggaccgtgtg
     7261 cacttcgctt cacctctgca cgtcgcatgg agaccaccgt gaacgcccac caaatattgc
     7321 ccaaggtctt acataagagg actcttggac tctcagcaat gtcaacgacc gaccttgagg
     7381 catacttcaa agactgtttg tttaaagact gggaggagtt gggggaggag attaggttaa
     7441 aggtctttgt actaggaggc tgtaggcata aattggtctg cgcaccagca ccatggcgca
     7501 atcactagag cggggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7561 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7621 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7681 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7741 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7801 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7861 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7921 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7981 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8041 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8101 actaattttt tttatttatg cagaggccga ggccggatcc cttgagtggc tttcatcctg
     8161 gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc
     8221 tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg
     8281 gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag
     8341 agggcattag caatagtgtt tataaggccc ccttgttaat tcttgaagac gaaagggcct
     8401 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8461 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8521 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8581 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8641 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8701 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8761 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8821 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8881 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8941 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9001 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9061 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9121 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9181 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9241 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9301 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9361 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9421 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9481 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9541 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9601 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9661 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9721 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     9781 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9841 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9901 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9961 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10021 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10081 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10141 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10201 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag
    10261 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
    10321 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcg
//
