LOCUS       sequence-413245-        2787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human SYK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2787)
  TITLE     TREAT-AD Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDEST-GST-hSYK
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             119..772
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     730..752
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2730..2754)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcctaggc cgcggaccat
      121 ggcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt
      181 ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg
      241 gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg
      301 tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat
      361 gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga
      421 tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga
      481 ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac
      541 atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt
      601 tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa
      661 acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg
      721 gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct
      781 ggttccgcgt tctagatcaa caagtttgta caaaaaagtt ggcaccatgg ccagcagcgg
      841 catggctgac agcgccaacc acctgccctt ctttttcggc aacatcaccc gggaggaggc
      901 agaagattac ctggtccagg ggggcatgag tgatgggctt tatttgctgc gccagagccg
      961 caactacctg ggtggcttcg ccctgtccgt ggcccacggg aggaaggcac accactacac
     1021 catcgagcgg gagctgaatg gcacctacgc catcgccggt ggcaggaccc atgccagccc
     1081 cgccgacctc tgccactacc actcccagga gtctgatggc ctggtctgcc tcctcaagaa
     1141 gcccttcaac cggccccaag gggtgcagcc caagactggg ccctttgagg atttgaagga
     1201 aaacctcatc agggaatatg tgaagcagac atggaacctg cagggtcagg ctctggagca
     1261 ggccatcatc agtcagaagc ctcagctgga gaagctgatc gctaccacag cccatgaaaa
     1321 aatgccttgg ttccatggaa aaatctctcg ggaagaatct gagcaaattg tcctgatagg
     1381 atcaaagaca aatggaaagt tcctgatccg agccagagac aacaacggct cctacgccct
     1441 gtgcctgctg cacgaaggga aggtgctgca ctatcgcatc gacaaagaca agacagggaa
     1501 gctctccatc cccgagggaa agaagttcga cacgctctgg cagctagtcg agcattattc
     1561 ttataaagca gatggtttgt taagagttct tactgtccca tgtcaaaaaa tcggcacaca
     1621 gggaaatgtt aattttggag gccgtccaca acttccaggt tcccatcctg cgacttggtc
     1681 agcgggtgga ataatctcaa gaatcaaatc atactccttc ccaaagcctg gccacagaaa
     1741 gtcctcccct gcccaaggga accggcaaga gagtactgtg tcattcaatc cgtatgagcc
     1801 agaacttgca ccctgggctg cagacaaagg cccccagaga gaagccctac ccatggacac
     1861 agaggtgtac gagagcccct acgcggaccc tgaggagatc aggcccaagg aggtttacct
     1921 ggaccgaaag ctgctgacgc tggaagacaa agaactgggc tctggtaatt ttggaactgt
     1981 gaaaaagggc tactaccaaa tgaaaaaagt tgtgaaaacc gtggctgtga aaatactgaa
     2041 aaacgaggcc aatgaccccg ctcttaaaga tgagttatta gcagaagcaa atgtcatgca
     2101 gcagctggac aacccgtaca tcgtgcgcat gatcgggata tgcgaggccg agtcctggat
     2161 gctagttatg gagatggcag aacttggtcc cctcaataag tatttgcagc agaacagaca
     2221 tgtcaaggat aagaacatca tagaactggt tcatcaggtt tccatgggca tgaagtactt
     2281 ggaggagagc aattttgtgc acagagatct ggctgcaaga aatgtgttgc tagttaccca
     2341 acattatgcc aagatcagtg atttcggact ctccaaagca ctgcgtgctg atgaaaacta
     2401 ctacaaggcc cagacccatg gaaagtggcc tgtcaagtgg tacgctccgg aatgcatcaa
     2461 ctactacaag ttctccagca aaagcgatgt ctggagcttt ggagtgttga tgtgggaagc
     2521 attctcctat gggcagaagc catatcgagg gatgaaagga agtgaagtca ccgctatgtt
     2581 agagaaagga gagcggatgg ggtgccctgc agggtgtcca agagagatgt acgatctcat
     2641 gaatctgtgc tggacatacg atgtggaaaa caggcccgga ttcgcagcag tggaactgcg
     2701 gctgcgcaat tactactatg acgtggtgaa cccaactttc ttgtacaaag tggttgatcg
     2761 cgtgcatgcg acgtcatagc tctctcc
//
