LOCUS       sequence-413246-        8573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EGFR in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8573)
  TITLE     Ullrich lab EGFR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRK5-EGFR
FEATURES             Location/Qualifiers
     source          1..8573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(1067..1138,1139..3001,3002..3070,3071..4699)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     regulatory      4873..4882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4886..5020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4923..4942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4977..4996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5089..5446
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5089..5280
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5112..5132)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5297..5432
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5466..5483)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5466..5482)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5475..5497)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5695..6150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5782..5801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5992..6013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6167..6186)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6286..6308
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6346..6364)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6432..6536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6537..6605,6606..7397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7568..8156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8057..8076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8310..8327
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8444..8465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8480..8485,8486..8503,8504..8510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8518..8534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8523..8545
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8542..8558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8542..8558
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgcgatt gaattccccg gggatcctct agagtcagct tatcgattct
      961 agccgagtcc ccgcctcgcc gccaacgcca caaccaccgc gcacggcccc ctgactccgt
     1021 ccagtattga tcgggagagc cggagcgagc tcttcgggga gcagcgatgc gaccctccgg
     1081 gacggccggg gcagcgctcc tggcgctgct ggctgcgctc tgcccggcga gtcgggctct
     1141 ggaggaaaag aaagtttgcc aaggcacgag taacaagctc acgcagttgg gcacttttga
     1201 agatcatttt ctcagcctcc agaggatgtt caataactgt gaggtggtcc ttgggaattt
     1261 ggaaattacc tatgtgcaga ggaattatga tctttccttc ttaaagacca tccaggaggt
     1321 ggctggttat gtcctcattg ccctcaacac agtggagcga attcctttgg aaaacctgca
     1381 gatcatcaga ggaaatatgt actacgaaaa ttcctatgcc ttagcagtct tatctaacta
     1441 tgatgcaaat aaaaccggac tgaaggagct gcccatgaga aatttacagg aaatcctgca
     1501 tggcgccgtg cggttcagca acaaccctgc cctgtgcaac gtggagagca tccagtggcg
     1561 ggacatagtc agcagtgact ttctcagcaa catgtcgatg gacttccaga accacctggg
     1621 cagctgccaa aagtgtgatc caagctgtcc caatgggagc tgctggggtg caggagagga
     1681 gaactgccag aaactgacca aaatcatctg tgcccagcag tgctccgggc gctgccgtgg
     1741 caagtccccc agtgactgct gccacaacca gtgtgctgca ggctgcacag gcccccggga
     1801 gagcgactgc ctggtctgcc gcaaattccg agacgaagcc acgtgcaagg acacctgccc
     1861 cccactcatg ctctacaacc ccaccacgta ccagatggat gtgaaccccg agggcaaata
     1921 cagctttggt gccacctgcg tgaagaagtg tccccgtaat tatgtggtga cagatcacgg
     1981 ctcgtgcgtc cgagcctgtg gggccgacag ctatgagatg gaggaagacg gcgtccgcaa
     2041 gtgtaagaag tgcgaagggc cttgccgcaa agtgtgtaac ggaataggta ttggtgaatt
     2101 taaagactca ctctccataa atgctacgaa tattaaacac ttcaaaaact gcacctccat
     2161 cagtggcgat ctccacatcc tgccggtggc atttaggggt gactccttca cacatactcc
     2221 tcctctggat ccacaggaac tggatattct gaaaaccgta aaggaaatca cagggttttt
     2281 gctgattcag gcttggcctg aaaacaggac ggacctccat gcctttgaga acctagaaat
     2341 catacgcggc aggaccaagc aacatggtca gttttctctt gcagtcgtca gcctgaacat
     2401 aacatccttg ggattacgct ccctcaagga gataagtgat ggagatgtga taatttcagg
     2461 aaacaaaaat ttgtgctatg caaatacaat aaactggaaa aaactgtttg ggacctccgg
     2521 tcagaaaacc aaaattataa gcaacagagg tgaaaacagc tgcaaggcca caggccaggt
     2581 ctgccatgcc ttgtgctccc ccgagggctg ctggggcccg gagcccaggg actgcgtctc
     2641 ttgccggaat gtcagccgag gcagggaatg cgtggacaag tgcaaccttc tggagggtga
     2701 gccaagggag tttgtggaga actctgagtg catacagtgc cacccagagt gcctgcctca
     2761 ggccatgaac atcacctgca caggacgggg accagacaac tgtatccagt gtgcccacta
     2821 cattgacggc ccccactgcg tcaagacctg cccggcagga gtcatgggag aaaacaacac
     2881 cctggtctgg aagtacgcag acgccggcca tgtgtgccac ctgtgccatc caaactgcac
     2941 ctacggatgc actgggccag gtcttgaagg ctgtccaacg aatgggccta agatcccgtc
     3001 catcgccact gggatggtgg gggccctcct cttgctgctg gtggtggccc tggggatcgg
     3061 cctcttcatg cgaaggcgcc acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga
     3121 gagggagctt gtggagcctc ttacacccag tggagaagct cccaaccaag ctctcttgag
     3181 gatcttgaag gaaactgaat tcaaaaagat caaagtgctg ggctccggtg cgttcggcac
     3241 ggtgtataag ggactctgga tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa
     3301 ggaattaaga gaagcaacat ctccgaaagc caacaaggaa atcctcgatg aagcctacgt
     3361 gatggccagc gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac
     3421 cgtgcaactc atcacgcagc tcatgccctt cggctgcctc ctggactatg tccgggaaca
     3481 caaagacaat attggctccc agtacctgct caactggtgt gtgcagatcg caaagggcat
     3541 gaactacttg gaggaccgtc gcttggtgca ccgcgacctg gcagccagga acgtactggt
     3601 gaaaacaccg cagcatgtca agatcacaga ttttgggctg gccaaactgc tgggtgcgga
     3661 agagaaagaa taccatgcag aaggaggcaa agtgcctatc aagtggatgg cattggaatc
     3721 aattttacac agaatctata cccaccagag tgatgtctgg agctacgggg tgaccgtttg
     3781 ggagttgatg acctttggat ccaagccata tgacggaatc cctgccagcg agatctcctc
     3841 catcctggag aaaggagaac gcctccctca gccacccata tgtaccatcg atgtctacat
     3901 gatcatggtc aagtgctgga tgatagacgc agatagtcgc ccaaagttcc gtgagttgat
     3961 catcgaattc tccaaaatgg cccgagaccc ccagcgctac cttgtcattc agggggatga
     4021 aagaatgcat ttgccaagtc ctacagactc caacttctac cgtgccctga tggatgaaga
     4081 agacatggac gacgtggtgg atgccgacga gtacctcatc ccacagcagg gcttcttcag
     4141 cagcccctcc acgtcacgga ctcccctcct gagctctctg agtgcaacca gcaacaattc
     4201 caccgtggct tgcattgata gaaatgggct gcaaagctgt cccatcaagg aagacagctt
     4261 cttgcagcga tacagctcag accccacagg cgccttgact gaggacagca tagacgacac
     4321 cttcctccca gtgcctgaat acataaacca gtccgttccc aaaaggcccg ctggctctgt
     4381 gcagaatcct gtctatcaca atcagcctct gaaccccgcg cccagcagag acccacacta
     4441 ccaggacccc cacagcactg cagtgggcaa ccccgagtat ctcaacactg tccagcccac
     4501 ctgtgtcaac agcacattcg acagccctgc ccactgggcc cagaaaggca gccaccaaat
     4561 tagcctggac aaccctgact accagcagga cttctttccc aaggaagcca agccaaatgg
     4621 catctttaag ggctccacag ctgaaaatgc agaataccta agggtcgcgc cacaaagcag
     4681 tgaatttatt ggagcatgac cacggaggat agtatgagcc ctaaaaatcc agactctttc
     4741 gatacccagg accaagccac agcaggtcct ccatcccaac agccatgccc gcattagctc
     4801 ttagacccac agactggttt tgcaacgttt acaccgacta gccaggaagt atccttcgcg
     4861 ggatcaagct tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata
     4921 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4981 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat tcggcgcagc
     5041 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
     5101 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5161 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5221 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5281 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5341 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5401 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc
     5461 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5521 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5581 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     5641 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     5701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5881 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     5941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6001 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     6061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6121 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     6181 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6241 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6301 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6361 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6421 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6481 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6541 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6601 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6661 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6721 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6781 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6841 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6901 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6961 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7021 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7081 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7141 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7201 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7261 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7321 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7381 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7441 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7501 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7561 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7621 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7681 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7741 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7801 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7861 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7921 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7981 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8041 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8101 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8161 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8221 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8281 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8341 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8401 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8461 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8521 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat taa
//
