LOCUS       sequence-413247-       10655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP10A mutant E203Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin HW
  TITLE     Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is
            Involved in Plasma Membrane Dynamics.
  JOURNAL   J Biol Chem. 2015 Jun 12;290(24):15004-17. doi: 
            10.1074/jbc.M115.655191. Epub 2015 May 6.
  PUBMED    25947375
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.655191"; journalName: "J Biol Chem"; date: 
            "2015-06-12- 12"; volume: "290"; issue: "24"; pages: "15004-17"
COMMENT     Sequence Label: pCAG/ATP10A(E203Q)-HA
FEATURES             Location/Qualifiers
     source          1..10655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1918..2934
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2858..2880
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2942..2961
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3030..3054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7609..7633)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7677..7703
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7791..7810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7856..7911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7910..7929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8272..8288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8272..8288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8285..8307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8296..8312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8320..8326,8327..8344,8345..8350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8365..8386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8445..8640
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8491..8626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8646..8780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8683..8702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8737..8756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8848..8865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9019..9607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9099..9118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9778..10569,10570..10638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10401..10420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10639..10655,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg ggcgccctgg gggggggggg ggggggggtg
     1801 gggggggggc gggggggggg ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     1861 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     1921 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     1981 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     2041 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     2101 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     2161 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     2221 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     2281 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     2341 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     2401 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     2461 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     2521 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     2581 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     2641 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     2701 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     2761 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     2821 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     2881 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     2941 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaatttgc tagcgagctc
     3001 gcggccgcag atctctagtt aagctatcaa caagtttgta caaaaaagca ggctctttaa
     3061 aggaaccaat tcagtcgact ggatccacca tggagcggga gccggcgggg accgaggagc
     3121 ccgggcctcc gggacggcgg aggcgccgag agggcaggac gcgcacggtg cgctccaacc
     3181 tgctgccgcc cccgggcgcc gaggaccctg cggctggcgc ggccaagggc gagcggcgac
     3241 ggcggcgcgg gtgtgcccag cacctggccg acaaccggct caagactacc aagtacacgc
     3301 tgctgtcctt cctgcccaag aacctgttcg agcagttcca ccgcccggcc aacgtgtact
     3361 ttgtcttcat cgcgctgctc aacttcgtgc cggcggtgaa cgccttccag cccggcctgg
     3421 cactggcgcc ggtgctcttc atcctggcca tcacggcctt cagggacctg tgggaggact
     3481 acagccgcca ccgctccgac cacaagatca accacctggg ctgcctggtc ttcagcaggg
     3541 aagaaaagaa atacgtgaac cgattctgga aagaaatcca cgtgggagac tttgtgcgtc
     3601 ttcgctgcaa cgaaatcttc cctgcggaca ttctgctgct ctcctccagt gaccccgacg
     3661 ggctatgcca catcgagacc gccaacctgg atggacagac caacctgaag cggcggcagg
     3721 tggtccgcgg cttctcggag cttgtctccg aattcaatcc tttgacgttc accagcgtga
     3781 tcgaatgcga gaagccaaac aacgacctga gtaggtttcg cggctgcatc atacatgaca
     3841 acgggaaaaa ggccgggctg tataaagaaa acctgctgct gaggggctgc acccttagga
     3901 acacggacgc agtcgtcggc attgtcatct acgcaggaca tgaaaccaag gctctgctga
     3961 acaacagtgg gccccgctac aagcgcagca agctggagag gcagatgaac tgcgacgtgc
     4021 tctggtgtgt cctgctcctt gtttgcatgt ctctgttttc agcagtcgga catggactgt
     4081 ggatatggcg gtatcaagag aagaagtcat tattttatgt ccccaagtct gatggaagct
     4141 ccttatcccc agtcacagct gcagtttact catttttaac aatgataata gttctgcagg
     4201 ttttgatccc aatttcctta tacgtttcca ttgaaattgt taaagcatgc caagtgtact
     4261 tcattaacca ggacatgcag ttgtatgacg aagaaacaga ctcgcagctg cagtgccgag
     4321 ctctgaacat cacggaagac ttaggacaga tacagtacat tttctcagat aaaactggca
     4381 ctttgacaga gaataagatg gttttccgaa gatgcactgt gtctggtgta gaatattctc
     4441 atgatgcaaa tgcgcagcgt ctggccaggt accaagaggc agactcggag gaggaggagg
     4501 tggtgcccag agggggctcg gtgtcccagc gcggcagcat cggcagccac cagagtgtcc
     4561 gggtggtgca cagaacccag agcaccaagt cccaccggcg cacgggcagc cgggccgagg
     4621 ccaagagggc cagcatgctg tccaagcaca cggccttcag cagccccatg gagaaggata
     4681 tcacgcccga cccaaagctg ctggagaagg tgagtgagtg tgacaagagc ctagccgtgg
     4741 cgaggcatca ggagcacctg ctggcccacc tctcgcccga gctgtctgac gtctttgatt
     4801 tcttcatcgc actcaccatc tgcaacacag tcgtcgtcac gtccccggat cagccacgaa
     4861 caaaggtgag ggtgaggttt gagctgaagt ccccggtgaa gacgatagaa gacttcctgc
     4921 ggaggttcac acccagctgc ctgacctcag gctgcagcag catcgggagc ctggccgcca
     4981 acaagtccag ccacaagttg ggctccagct tcccgtccac cccgtccagc gacggcatgc
     5041 ttctcaggct ggaggagagg ctgggccagc ccacctcggc catcgccagc aacggctaca
     5101 gcagccaggc ggacaactgg gcctcggagc ttgctcagga gcaggagtca gagcgcgagc
     5161 tgcggtacga ggcggagagc ccggatgagg ccgcactggt gtatgcggcc agagcctaca
     5221 actgcgtgct tgtggagcgg ctgcacgacc aagtgtcagt ggagctgccc cacctgggca
     5281 ggctcacctt cgagctcctg cacacactgg gtttcgattc cgtccgcaag aggatgtcag
     5341 tggtgatccg gcacccgctt accgatgaga tcaacgtcta caccaagggg gccgactcag
     5401 tggtcatgga tctcctgcag ccctgctctt cagttgacgc cagagggagg catcaaaaaa
     5461 agattcggag caaaactcag aattacctca acgtgtatgc ggcggaaggc ctgcgcacct
     5521 tgtgcatcgc caagagagtt ctgagtaaag aagagtatgc ctgctggttg caaagccacc
     5581 tagaagccga atcctccctg gaaaacagcg aggagctcct cttccagtct gccattcgcc
     5641 tggagaccaa cctgcacttg ttaggtgcca ctgggattga agaccgcctg caggacggag
     5701 tccctgaaac tatttctaaa ttgcgtcaag cgggcctgca gatttgggtt ctcactggtg
     5761 acaaacaaga aacagctgtc aacattgcat atgcctgcaa actgctggac cacgacgagg
     5821 aggtcatcac cctgaatgcc acctcccagg aggcgtgtgc agccctgcta gaccagtgcc
     5881 tatgctacgt gcagtccaga ggcctccaga gagcccctga gaagaccaag ggcaaagtga
     5941 gcatgaggtt ctcctctctc tgcccaccct ccacgtccac tgcctctggc cgcagaccca
     6001 gcctcgtgat cgatgggaga agcctggcct acgctctcga gaaaaacctg gaggacaaat
     6061 tcctcttcct tgccaagcag tgccgctccg tcctctgctg tcggtcgacg cctctgcaga
     6121 agagcatggt ggtgaagctg gtgcggagca agctcaaggc catgaccctg gccataggtg
     6181 atggagccaa tgatgtcagc atgatccagg tggcagatgt gggtgtggga atctccggcc
     6241 aggagggtat gcaggcagtg atggccagcg actttgcagt gccgaaattc cgatacctgg
     6301 agaggctctt gattcttcac gggcattggt gctactcccg acttgccaac atggtgctgt
     6361 acttcttcta caaaaacaca atgttcgtgg gcctcctgtt ttggttccag tttttctgtg
     6421 gcttctctgc atctaccatg attgaccagt ggtatctaat cttctttaat ctgctcttct
     6481 cgtcacttcc cccgctcgtg actggggtgc tggacaggga tgtgccagcc aatgtgctgc
     6541 tgaccaaccc gcagctctac aagagtggcc agaacatgga ggaataccgg ccacgaacgt
     6601 tctggtttaa catggccgac gccgccttcc agagcctggt ttgcttttcc attccttacc
     6661 tggcctacta tgactcgaac gtggacctgt ttacctgggg gacccctatt gtgacaatcg
     6721 cgctgctcac tttcctgctc cacctgggca ttgaaaccaa aacctggacc tggctcaact
     6781 ggataacgtg tggcttcagt gtccttttgt ttttcaccgt ggctttgatt tacaatgcgt
     6841 cttgtgccac gtgctatcct ccgtccaacc cttactggac tatgcaagcc ttactgggtg
     6901 acccagtgtt ttacttgact tgcctgatga cgcctgtcgc tgcactgctg cccagattgt
     6961 ttttcagatc cctccagggg agggttttcc ccacacaact tcagctggca cgtcagttga
     7021 ccaggaagtc ccccaggaga tgcagtgctc ccaaagagac ctttgctcag ggacgcctcc
     7081 cgaaggactc gggaaccgag cactcatcag ggaggacagt caagacctct gtgcccctgt
     7141 cccagccttc ttggcacaca cagcagccgg tctgctccct ggaggccagc ggggagccca
     7201 gcacagtgga catgagcatg ccagtgaggg agcacaccct gctggagggg ctgagcgcac
     7261 cggcccccat gtcctctgcg ccaggggagg ctgtcctgag gagtccagga gggtgtcctg
     7321 aggagtccaa ggtgagagct gccagcaccg gcagggtgac ccccctgtct tccctcttca
     7381 gcctgcctac cttcagctta ctcaactgga tttcctcctg gtcgctggtc agcaggctgg
     7441 ggagtgtctt acagttctcc cggacggagc agcttgcaga tggacaagcg ggacgtggac
     7501 ttcctgtcca gccccactca ggccgatcag gacttcaagg gccagaccac agactactta
     7561 taggagcatc ttcaaggcgg tcacagcggc cgcactcgag atatctagac ccagctttct
     7621 tgtacaaagt ggttgatcta gagggcccgc ggttcgaact cgaggtaccg aattcgtacc
     7681 catacgacgt cccagactac gcttaattca ctcctcaggt gcaggctgcc tatcagaagg
     7741 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatcga tctttttccc
     7801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     7861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     7921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     7981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     8041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     8101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     8161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     8221 cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct
     8281 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8341 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8401 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca
     8461 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8521 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8581 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8641 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8701 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8761 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8821 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8881 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8941 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9001 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9061 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10621 gaaatgttga atactcatac tcttcctttt tcaat
//
