LOCUS       sequence-413249-        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FCER1G in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  TITLE     TREAT-AD Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3-hFCER1G-VenusFlag
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(394..418)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             429..452
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(483..485,486..488,489..1202)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(528..549)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(789..808)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1136..1157
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttgctagtta agctagcaac aagtttgtac aaaaaagcag
      121 gctccaccat gattccagca gtggtcttgc tcttactcct tttggttgaa caagcagcgg
      181 ccctgggaga gcctcagctc tgctatatcc tggatgccat cctgtttctg tatggaattg
      241 tcctcaccct cctctactgt cgactgaagg taatccaagt gcgaaaggca gctataacca
      301 gctatgagaa atcagatggt gtttacacgg gcctgagcac caggaaccag gagacttacg
      361 agactctgaa gcatgagaaa ccaccacagt tggacccagc tttcttgtac aaagtggttg
      421 atctcgagga ctacaaggac gacgatgaca agggaggggg tggatctgga gggggtggat
      481 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      541 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      601 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
      661 ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      721 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      781 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      841 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      901 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
      961 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     1021 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1081 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     1141 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     1201 aaggtac
//
