LOCUS       sequence-413252-       10600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP10B mutant E210Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin HW
  TITLE     Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is
            Involved in Plasma Membrane Dynamics.
  JOURNAL   J Biol Chem. 2015 Jun 12;290(24):15004-17. doi: 
            10.1074/jbc.M115.655191. Epub 2015 May 6.
  PUBMED    25947375
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.655191"; journalName: "J Biol Chem"; date: 
            "2015-06-12- 12"; volume: "290"; issue: "24"; pages: "15004-17"
COMMENT     Sequence Label: pCAG/ATP10B(E210Q)-HA
FEATURES             Location/Qualifiers
     source          1..10600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     366..388
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     450..469
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    538..562
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1645..1656)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5006..5030)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5074..5100
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5188..5207)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5253..5308
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5307..5326)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5669..5685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5669..5685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5682..5704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5693..5709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5717..5723,5724..5741,5742..5747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5762..5783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5842..6037
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5888..6023
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6043..6177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6080..6099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6134..6153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6245..6262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6416..7004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6496..6515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7175..7966,7967..8035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7798..7817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8036..8140)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8452..9255
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8515..8534)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9125..9144
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9262..9310
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9320..9699
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          join(10026..10600,1..442)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
ORIGIN
        1 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
       61 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
      121 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
      181 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
      241 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
      301 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
      361 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
      421 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
      481 ttttggcaaa gaatttgcta gcgagctcgc ggccgcagat ctctagttaa gctatcaaca
      541 agtttgtaca aaaaagcagg ctctttaaag gaaccaattc agtcgactgg atccggtacc
      601 gaattcacca tggccctctc agtggactca tcgtggcatc ggtggcagtg gagagtcaga
      661 gatggcttcc cccattgtcc atcggaaacc acaccgctgc tctctccaga gaaagggaga
      721 cagagctaca acttgacaca gcagcgggtc gtgttcccca acaacagcat attccatcaa
      781 gattgggaag aggtctccag gagataccct ggcaacagaa cctgcacaac caaatacacc
      841 ctcttcacct tcctgccccg gaatctcttt gagcaatttc atagatgggc taacctctat
      901 ttcctgttcc tggtgatttt gaactggatg ccctccatgg aagtcttcca cagagaaatc
      961 accatgttac cattggccat tgtcctgttc gtcatcatga tcaaggatgg catggaggac
     1021 ttcaagagac accgctttga taaagcaata aactgctcca acattcgaat ttatgaaaga
     1081 aaagagcaga cctatgtgca gaagtgctgg aaggatgtgc gtgtgggaga cttcatccaa
     1141 atgaaatgca atgagattgt cccagcagac atactcctcc ttttttcctc tgaccccaat
     1201 gggatatgcc atctggaaac tgccagcttg gatggacaga caaacctcaa gcaaagacgt
     1261 gtcgtgaagg gcttctcaca gcaggaggta cagttcgaac cagagctttt ccacaatacc
     1321 atcgtgtgtg agaaacccaa caaccacctc aacaaattta agggttatat ggagcatcct
     1381 gaccagacca ggactggctt tggctgtgag agtcttctgc ttcgaggctg caccatcaga
     1441 aacaccgaga tggctgttgg cattgtcatc tatgcaggcc atgagacgaa agccatgctg
     1501 aacaacagtg gcccccggta caaacgcagc aagattgagc ggcgcatgaa tatagacatc
     1561 ttcttctgca ttgggatcct catcctcatg tgccttattg gagctgtagg tcacagcatc
     1621 tggaatggga cctttgaaga acaccctccc ttcgatgtgc cagatgccaa tggcagcttc
     1681 cttcccagtg cccttggggg cttctacatg ttcctcacaa tgatcatcct gctccaggtg
     1741 ctgatcccca tctctttgta tgtctccatt gagctggtga agctcgggca agtgttcttc
     1801 ttgagcaatg accttgacct gtatgatgaa gagaccgatt tatccattca atgtcgagcc
     1861 ctcaacatcg cagaggactt gggccagatc cagtacatct tctccgataa gacggggacc
     1921 ctgacagaga acaagatggt gttccgacgt tgcaccatca tgggcagcga gtattctcac
     1981 caagaaaatg ctaagcgact ggagacccca aaggagctgg actcagatgg tgaagagtgg
     2041 acccaatacc aatgcctgtc cttctcggct agatgggccc aggatccagc aactatgaga
     2101 agccaaaaag gtgctcagcc tctgaggagg agccagagtg cccgggtgcc catccagggc
     2161 cactaccggc aaaggtctat ggggcaccgt gaaagctcac agcctcctgt ggccttcagc
     2221 agctccatag aaaaagatgt aactccagat aaaaacctac tgaccaaggt tcgagatgct
     2281 gccctgtggt tggagacctt gtcagacagc agacctgcca aggcttccct ctccaccacc
     2341 tcctccattg ctgatttctt ccttgcctta accatctgca actctgtcat ggtgtccaca
     2401 accaccgagc ccaggcagag ggtcaccatc aaaccctcaa gcaaggctct ggggacgtcc
     2461 ctggagaaga ttcagcagct cttccagaag ttgaagctat tgagcctcag ccagtcattc
     2521 tcatccactg caccctctga cacagacctc ggggagagct taggggccaa cgtggccacc
     2581 acagactcgg atgagagaga tgatgcatct gtgtgcagtg gaggtgactc cactgatgac
     2641 ggtggctaca ggagcagcat gtgggaccag ggcgacatcc tggagtctgg gtcaggcact
     2701 tccttggagg aggcattgga ggccccagcc acagacctgg ccaggcctga gttctgttac
     2761 gaggctgaga gccctgatga ggccgccctg gtgcacgctg cccatgccta cagcttcaca
     2821 ctagtgtccc ggacacctga gcaggtgact gtgcgcctgc cccagggcac ctgcctcacc
     2881 ttcagcctcc tctgcaccct gggctttgac tctgtcagga agagaatgtc tgtggttgtg
     2941 aggcacccac tgactggcga gattgttgtc tacaccaagg gtgctgactc ggtcatcatg
     3001 gacctgctgg aagacccagc ctgcgtacct gacattaata tggaaaagaa gctgagaaaa
     3061 atccgagccc ggacccaaaa gcatctagac ttgtatgcaa gagatggcct gcgcacacta
     3121 tgcattgcca agaaggttgt aagcgaagag gacttccgga gatgggccag tttccggcgt
     3181 gaggctgagg catccctcga caaccgagat gagcttctca tggaaactgc acagcatctg
     3241 gagaatcaac tcaccttact tggagccact gggatcgaag accggctgca ggaaggagtt
     3301 ccagatacga ttgccactct gcgggaggct gggatccagc tctgggtcct gactggagat
     3361 aagcaggaga cagcggtcaa cattgcccat tcctgcagac tgttaaatca gaccgacact
     3421 gtttatacca tcaatacaga gaatcaggag acctgtgaat ccatcctcaa ttgtgcattg
     3481 gaagagctaa agcaatttcg tgaactacag aagccagacc gcaagctctt tggattccgc
     3541 ttaccttcca agacaccatc catcacctca gaagctgtgg ttccagaagc tggattggtc
     3601 atcgatggga agacattgaa tgccatcttc cagggaaagc tagagaagaa gtttctggaa
     3661 ttgacccagt attgtcggtc cgtcctgtgc tgccgctcca cgccactcca gaagagtatg
     3721 atagtcaagc tggtgcgaga caagttgcgc gtcatgaccc tttccatagg tgatggagca
     3781 aatgatgtaa gcatgattca agctgctgat attggaattg gaatatctgg acaggaaggc
     3841 atgcaggctg tcatgtccag cgactttgcc atcacccgct ttaagcatct caagaagttg
     3901 ctgctcgtgc atggccactg gtgttactcg cgcctggcca ggatggtggt gtactacctc
     3961 tacaagaacg tgtgctacgt caacctgctc ttctggtatc agttcttctg tggtttctcc
     4021 agctccacca tgattgatta ctggcagatg atattcttca atctcttctt tacctccttg
     4081 cctcctcttg tctttggagt ccttgacaaa gacatctctg cagaaacact cctggcattg
     4141 cctgagctat acaagagtgg ccagaactct gagtgctata acctgtcgac tttctggatt
     4201 tctatggtgg atgcattcta ccagagcctc atctgtttct ttatccctta cctggcctat
     4261 aagggctctg atatagatgt ctttaccttt gggacaccaa tcaacaccat ctccctcacc
     4321 acaatccttt tgcaccaggc aatggaaatg aagacatgga ccattttcca cggagtcgtg
     4381 ctcctcggca gcttcctgat gtactttctg gtatccctcc tgtacaatgc cacctgcgtc
     4441 atctgcaaca gccccaccaa tccctattgg gtgatggaag gccagctctc aaaccccact
     4501 ttctacctcg tctgctttct cacaccagtt gttgctcttc tcccaagata ctttttcctg
     4561 tctctgcaag gaacttgtgg gaagtctcta atctcaaaag ctcagaaaat tgacaaactc
     4621 cccccagaca aaagaaacct ggaaatccag agttggagaa gcagacagag gcctgcccct
     4681 gtccccgaag tggctcgacc aactcaccac ccagtgtcat ctatcacagg acaggacttc
     4741 agtgccagca ccccaaagag ctctaaccct cccaagagga agcatgtgga agagtcagta
     4801 ctccacgaac agagatgtgg cacggagtgc atgagggatg actcatgctc aggggactcc
     4861 tcagctcaac tctcatccgg ggagcacctg ctgggaccta acaggataat ggcctactca
     4921 agaggacaga ctgatatgtg ccggtgctca aagaggagca gccatcgccg atcccagagt
     4981 tcactgacca tctcgagata tctagaccca gctttcttgt acaaagtggt tgatctagag
     5041 ggcccgcggt tcgaactcga ggtaccgaat tcgtacccat acgacgtccc agactacgct
     5101 taattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     5161 ctggctcaca aataccactg agatcgatct ttttccctct gccaaaaatt atggggacat
     5221 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5281 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5341 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5401 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5461 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5521 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5581 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     5641 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5821 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5881 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5941 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6001 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     6061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     6181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6241 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6301 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6361 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6421 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6481 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6541 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6601 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6661 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6721 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6781 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6841 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6901 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6961 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7021 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7081 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7141 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7201 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7261 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7321 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7381 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7441 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7501 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7561 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7621 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7681 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7741 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7801 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7861 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7921 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7981 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8041 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8101 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8161 cacctgggtc gagcagtgtg gttttcaaga ggaagcaaaa agcctctcca cccaggcctg
     8221 gaatgtttcc acccaatgtc gagcagtgtg gttttgcaag aggaagcaaa aagcctctcc
     8281 acccaggcct ggaatgtttc cacccaatgt cgagcaaacc ccgcccagcg tcttgtcatt
     8341 ggcgaattaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc
     8401 atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagccaa tatgggatcg
     8461 gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     8521 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     8581 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     8641 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     8701 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     8761 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     8821 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     8881 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     8941 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     9001 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     9061 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     9121 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     9181 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     9241 gacgagttct tctgagggga tcggcaataa aaagacagaa taaaacgcac gggtgttggg
     9301 tcgtttgttc ggatccgtcg acattgatta ttgactagtt attaatagta atcaattacg
     9361 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9481 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9541 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9601 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9661 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     9721 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     9781 taattatttt gtgcagcgat gggggcgggg gggggggggg ggggcgcgcc gggggggggg
     9841 gggggggggg gggggggggg gggggggggg gggggggggg gggggcgggg cggggagagg
     9901 gggggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     9961 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
    10021 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
    10081 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
    10141 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
    10201 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
    10261 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
    10321 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
    10381 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
    10441 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
    10501 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
    10561 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
//
