LOCUS       sequence-413274-       10451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP10D mutant E215Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin HW
  TITLE     Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is
            Involved in Plasma Membrane Dynamics.
  JOURNAL   J Biol Chem. 2015 Jun 12;290(24):15004-17. doi: 
            10.1074/jbc.M115.655191. Epub 2015 May 6.
  PUBMED    25947375
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.655191"; journalName: "J Biol Chem"; date: 
            "2015-06-12- 12"; volume: "290"; issue: "24"; pages: "15004-17"
COMMENT     Sequence Label: pCAG/ATP10D(E215Q)-HA
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1942..2958
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2882..2904
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2966..2985
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3054..3078
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7405..7429)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7473..7499
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7587..7606)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7652..7707
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7706..7725)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8068..8084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8068..8084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8081..8103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8092..8108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8116..8122,8123..8140,8141..8146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8161..8182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8241..8436
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8287..8422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8442..8576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8479..8498)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8533..8552
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8644..8661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8815..9403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8895..8914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9574..10365,10366..10434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10197..10216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10435..10451,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg gggggggggg gggggggggg gggggggggg
     1801 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag
     1861 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
     1921 aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc
     1981 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     2041 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
     2101 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
     2161 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
     2221 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
     2281 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
     2341 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
     2401 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
     2461 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
     2521 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc
     2581 ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     2641 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa
     2701 tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc
     2761 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct
     2821 ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
     2881 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
     2941 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     3001 ggcaaagaat ttgctagcga gctcgcggcc gcagatctct agttaagcta tcaacaagtt
     3061 tgtacaaaaa agcaggctct ttaaaggaac caattcagtc gactggatcc accatgactg
     3121 aggctctcca atgggccaga tatcactggc gacggctgat cagaggtgca accagggatg
     3181 atgattcagg gccatacaac tattcctcgt tgctcgcctg tgggcgcaag tcctctcaga
     3241 tccctaaact gtcaggaagg caccggattg ttgttcccca catccagccc ttcaaggatg
     3301 agtatgagaa gttctccgga gcctatgtga acaatcgaat acgaacaaca aagtacacac
     3361 ttctgaattt tgtgccaaga aatttatttg aacaatttca cagagctgcc aatttatatt
     3421 tcctgttcct agttgtcctg aactgggtac ctttggtaga agccttccaa aaggaaatca
     3481 ccatgttgcc tctggtggtg gtccttacaa ttatcgcaat taaagatggc ctggaagatt
     3541 atcggaaata caaaattgac aaacagatca ataatttaat aactaaagtt tatagtagga
     3601 aagagaaaaa atacattgac cgatgctgga aagacgttac tgttggggac tttattcgcc
     3661 tctcctgcaa cgaggtcatc cctgcagaca tggtactact cttttccact gatccagatg
     3721 gaatctgtca cattgagact tctggtcttg atggacagag caatttaaaa cagaggcagg
     3781 tggttcgggg atatgcagaa caggactctg aagttgatcc tgagaagttt tccagtagga
     3841 tagaatgtga aagcccaaac aatgacctca gcagattccg aggcttccta gaacattcca
     3901 acaaagaacg cgtgggtctc agtaaagaaa atttgttgct tagaggatgc accattagaa
     3961 acacagaggc tgttgtgggc attgtggttt atgcaggcca tgaaaccaaa gcaatgctga
     4021 acaacagtgg gccacggtat aagcgcagca aattagaaag aagagcaaac acagatgtcc
     4081 tctggtgtgt catgcttctg gtcataatgt gcttaactgg cgcagtaggt catggaatct
     4141 ggctgagcag gtatgaaaag atgcattttt tcaatgttcc cgagcctgat ggacatatca
     4201 tatcaccact gttggcagga ttttatatgt tttggaccat gatcattttg ttacaggtct
     4261 tgattcctat ttctctctat gtttccatcg aaattgtgaa gcttggacaa atatatttca
     4321 ttcaaagtga tgtggatttc tacaatgaaa aaatggattc tattgttcag tgccgagccc
     4381 tgaacatcgc cgaggatctg ggacagattc agtacctctt ttccgataag acaggaaccc
     4441 tcactgagaa taagatggtt tttcgaagat gtagtgtggc aggatttgat tactgccatg
     4501 aagaaaatgc caggaggttg gagtcctatc aggaagctgt ctctgaagat gaagatttta
     4561 tagacacagt cagtggttcc ctcagcaata tggcaaaacc gagagccccc agctgcagga
     4621 cagttcataa tgggcctttg ggaaataagc cctcaaatca tcttgctggg agctctttta
     4681 ctctaggaag tggagaagga gccagtgaag tgcctcattc cagacaggct gctttcagta
     4741 gccccattga aacagacgtg gtaccagaca ccaggctttt agacaaattt agtcagatta
     4801 cacctcggct ctttatgcca ctagatgaga ccatccaaaa tccaccaatg gaaactttgt
     4861 acattatcga ctttttcatt gcattggcaa tttgcaacac agtagtggtt tctgctccta
     4921 accaaccccg acaaaagatc agacaccctt cactgggggg gttgcccatt aagtctttgg
     4981 aagagattaa aagtcttttc cagagatggt ctgtccgaag atcaagttct ccatcgctta
     5041 acagtgggaa agagccatct tctggagttc caaacgcctt tgtgagcaga ctccctctct
     5101 ttagtcgaat gaaaccagct tcacctgtgg aggaagaggt ctcccaggtg tgtgagagcc
     5161 cccagtgctc cagtagctca gcttgctgca cagaaacaga gaaacaacac ggtgatgcag
     5221 gcctcctgaa tggcaaggca gagtccctcc ctggacagcc attggcctgc aacctgtgtt
     5281 atgaggccga gagcccagac gaagcggcct tagtgtatgc cgccagggct taccaatgca
     5341 ctttacggtc tcggacacca gagcaggtca tggtggactt tgctgctttg ggaccattaa
     5401 catttcaact cctacacatc ctgccctttg actcagtaag aaaaagaatg tctgttgtgg
     5461 tccgacaccc tctttccaat caagttgtgg tgtatacgaa aggcgctgat tctgtgatca
     5521 tggagttact gtcggtggct tccccagatg gagcaagtct ggagaaacaa cagatgatag
     5581 taagggagaa aacccagaag cacttggatg actatgccaa acaaggcctt cgtactttat
     5641 gtatagcaaa gaaggtcatg agtgacactg aatatgcaga gtggctgagg aatcattttt
     5701 tagctgaaac cagcattgac aacagggaag aattactact tgaatctgcc atgaggttgg
     5761 agaacaaact tacattactt ggtgctactg gcattgaaga ccgtctgcag gagggagtcc
     5821 ctgaatctat agaagctctt cacaaagcgg gcatcaagat ctggatgctg acaggggaca
     5881 agcaggagac agctgtcaac atagcttatg catgcaaact actggagcca gatgacaagc
     5941 tttttatcct caatacccaa agtaaagatg cctgtgggat gctgatgagc acaattttga
     6001 aagaacttca gaagaaaact caagccctgc cagagcaagt gtcattaagt gaagatttac
     6061 ttcagcctcc tgtcccccgg gactcagggt tacgagctgg actcattatc actgggaaga
     6121 ccctggagtt tgccctgcaa gaaagtctgc aaaagcagtt cctggaactg acatcttggt
     6181 gtcaagctgt ggtctgctgc cgagccacac cgctgcagaa aagtgaagtg gtgaaattgg
     6241 tccgcagcca tctccaggtg atgacccttg ctattggtga tggtgccaat gatgttagca
     6301 tgatacaagt ggcagacatt gggatagggg tctcaggtca agaaggcatg caggctgtga
     6361 tggccagtga ctttgccgtt tctcagttca aacatctcag caagctcctt cttgtccatg
     6421 gacactggtg ttatacacgg ctttccaaca tgattctcta ttttttctat aagaatgtgg
     6481 cctatgtgaa cctccttttc tggtaccagt tcttttgtgg attttcagga acatccatga
     6541 ctgattactg ggttttgatc ttcttcaacc tcctcttcac atctgcccct cctgtcattt
     6601 atggtgtttt ggagaaagat gtgtctgcag agaccctcat gcaactgcct gaactttaca
     6661 gaagtggtca gaaatcagag gcatacttac cccatacctt ctggatcacc ttattggatg
     6721 ctttttatca aagcctggtc tgcttctttg tgccttattt tacctaccag ggctcagata
     6781 ctgacatctt tgcatttgga aaccccctga acacagccgc tctgttcatc attctcctcc
     6841 atctggtcat tgaaagcaag agtttgactt ggattcactt gctggtcatc attggtagca
     6901 tcttgtctta ttttttattt gccatagttt ttggagccat gtgtgtaact tgcaacccac
     6961 catccaaccc ttactggatt atgcaggagc acatgctgga tccagtattc tacttagttt
     7021 gtatcctcac gacgtccatt gctcttctgc ccaggtttgt atacagagtt cttcagggat
     7081 ccctgtttcc atctccaatt ctgagagcta agcactttga cagactaact ccagaggaga
     7141 ggactaaagc tctcaagaag tggagagggg ctggaaagat gaatcaagtg acatcaaagt
     7201 atgctaacca atcagctggc aagtcaggaa gaagacccat gcctggccct tctgctgtat
     7261 ttgcaatgaa gtcagcaagt tcctgtgcta ttgagcaagg aaacttatct ctgtgtgaaa
     7321 ctgctttaga tcaaggctac tctgaaacta aggcctttga gatggctgga ccctccaaag
     7381 gtaaagaaag ctcgagatat ctagacccag ctttcttgta caaagtggtt gatctagagg
     7441 gcccgcggtt cgaactcgag gtaccgaatt cgtacccata cgacgtccca gactacgctt
     7501 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     7561 tggctcacaa ataccactga gatcgatctt tttccctctg ccaaaaatta tggggacatc
     7621 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     7681 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     7741 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     7801 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     7861 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     7921 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     7981 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     8041 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8101 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8161 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8221 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8281 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8341 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8401 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     8461 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8521 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     8581 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8641 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8701 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10441 cctttttcaa t
//
