LOCUS       sequence-41328-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG6c
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    299..420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(310..329)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     364..383
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        553..657
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(876..895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctgc atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 ctataccggt ctcgatatca ctactagtgt tctagaattg ttaatctaaa ggttcaaaaa
      181 ctcacatatt tttcacacag tgtccaaatt tcatttgtac gataatacat tgttagttag
      241 ttttcatttt catattcatt tttcgtaaac attcaacggc cgcttcgagc agacatgata
      301 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      361 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      421 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      481 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccagg tggcactttt
      541 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      601 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      661 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      721 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      781 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      841 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      901 attgacgccg ggcaagagca cctcggtcgc cgcatacact attctcagaa tgacctggtt
      961 gagtactcac
//
