LOCUS       sequence-413280-       10941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP10A mutant E203Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin HW
  TITLE     Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is
            Involved in Plasma Membrane Dynamics.
  JOURNAL   J Biol Chem. 2015 Jun 12;290(24):15004-17. doi: 
            10.1074/jbc.M115.655191. Epub 2015 May 6.
  PUBMED    25947375
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.655191"; journalName: "J Biol Chem"; date: 
            "2015-06-12- 12"; volume: "290"; issue: "24"; pages: "15004-17"
COMMENT     Sequence Label: pMx/ATP10A(E203Q)-HA
FEATURES             Location/Qualifiers
     source          1..10941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6517..6541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6573..6599
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6768..6788)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6773..7102
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6953..7088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7459..8253
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7513..7532)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8123..8142
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8446..8915
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9045..9062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9216..9804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9296..9315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9968..10759,10760..10828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10591..10610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10829..10933)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ctctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccaccatg gagcgggagc cggcggggac cgaggagccc gggcctccgg
     2041 gacggcggag gcgccgagag ggcaggacgc gcacggtgcg ctccaacctg ctgccgcccc
     2101 cgggcgccga ggaccctgcg gctggcgcgg ccaagggcga gcggcgacgg cggcgcgggt
     2161 gtgcccagca cctggccgac aaccggctca agactaccaa gtacacgctg ctgtccttcc
     2221 tgcccaagaa cctgttcgag cagttccacc gcccggccaa cgtgtacttt gtcttcatcg
     2281 cgctgctcaa cttcgtgccg gcggtgaacg ccttccagcc cggcctggca ctggcgccgg
     2341 tgctcttcat cctggccatc acggccttca gggacctgtg ggaggactac agccgccacc
     2401 gctccgacca caagatcaac cacctgggct gcctggtctt cagcagggaa gaaaagaaat
     2461 acgtgaaccg attctggaaa gaaatccacg tgggagactt tgtgcgtctt cgctgcaacg
     2521 aaatcttccc tgcggacatt ctgctgctct cctccagtga ccccgacggg ctatgccaca
     2581 tcgagaccgc caacctggat ggacagacca acctgaagcg gcggcaggtg gtccgcggct
     2641 tctcggagct tgtctccgaa ttcaatcctt tgacgttcac cagcgtgatc gaatgcgaga
     2701 agccaaacaa cgacctgagt aggtttcgcg gctgcatcat acatgacaac gggaaaaagg
     2761 ccgggctgta taaagaaaac ctgctgctga ggggctgcac ccttaggaac acggacgcag
     2821 tcgtcggcat tgtcatctac gcaggacatg aaaccaaggc tctgctgaac aacagtgggc
     2881 cccgctacaa gcgcagcaag ctggagaggc agatgaactg cgacgtgctc tggtgtgtcc
     2941 tgctccttgt ttgcatgtct ctgttttcag cagtcggaca tggactgtgg atatggcggt
     3001 atcaagagaa gaagtcatta ttttatgtcc ccaagtctga tggaagctcc ttatccccag
     3061 tcacagctgc agtttactca tttttaacaa tgataatagt tctgcaggtt ttgatcccaa
     3121 tttccttata cgtttccatt gaaattgtta aagcatgcca agtgtacttc attaaccagg
     3181 acatgcagtt gtatgacgaa gaaacagact cgcagctgca gtgccgagct ctgaacatca
     3241 cggaagactt aggacagata cagtacattt tctcagataa aactggcact ttgacagaga
     3301 ataagatggt tttccgaaga tgcactgtgt ctggtgtaga atattctcat gatgcaaatg
     3361 cgcagcgtct ggccaggtac caagaggcag actcggagga ggaggaggtg gtgcccagag
     3421 ggggctcggt gtcccagcgc ggcagcatcg gcagccacca gagtgtccgg gtggtgcaca
     3481 gaacccagag caccaagtcc caccggcgca cgggcagccg ggccgaggcc aagagggcca
     3541 gcatgctgtc caagcacacg gccttcagca gccccatgga gaaggatatc acgcccgacc
     3601 caaagctgct ggagaaggtg agtgagtgtg acaagagcct agccgtggcg aggcatcagg
     3661 agcacctgct ggcccacctc tcgcccgagc tgtctgacgt ctttgatttc ttcatcgcac
     3721 tcaccatctg caacacagtc gtcgtcacgt ccccggatca gccacgaaca aaggtgaggg
     3781 tgaggtttga gctgaagtcc ccggtgaaga cgatagaaga cttcctgcgg aggttcacac
     3841 ccagctgcct gacctcaggc tgcagcagca tcgggagcct ggccgccaac aagtccagcc
     3901 acaagttggg ctccagcttc ccgtccaccc cgtccagcga cggcatgctt ctcaggctgg
     3961 aggagaggct gggccagccc acctcggcca tcgccagcaa cggctacagc agccaggcgg
     4021 acaactgggc ctcggagctt gctcaggagc aggagtcaga gcgcgagctg cggtacgagg
     4081 cggagagccc ggatgaggcc gcactggtgt atgcggccag agcctacaac tgcgtgcttg
     4141 tggagcggct gcacgaccaa gtgtcagtgg agctgcccca cctgggcagg ctcaccttcg
     4201 agctcctgca cacactgggt ttcgattccg tccgcaagag gatgtcagtg gtgatccggc
     4261 acccgcttac cgatgagatc aacgtctaca ccaagggggc cgactcagtg gtcatggatc
     4321 tcctgcagcc ctgctcttca gttgacgcca gagggaggca tcaaaaaaag attcggagca
     4381 aaactcagaa ttacctcaac gtgtatgcgg cggaaggcct gcgcaccttg tgcatcgcca
     4441 agagagttct gagtaaagaa gagtatgcct gctggttgca aagccaccta gaagccgaat
     4501 cctccctgga aaacagcgag gagctcctct tccagtctgc cattcgcctg gagaccaacc
     4561 tgcacttgtt aggtgccact gggattgaag accgcctgca ggacggagtc cctgaaacta
     4621 tttctaaatt gcgtcaagcg ggcctgcaga tttgggttct cactggtgac aaacaagaaa
     4681 cagctgtcaa cattgcatat gcctgcaaac tgctggacca cgacgaggag gtcatcaccc
     4741 tgaatgccac ctcccaggag gcgtgtgcag ccctgctaga ccagtgccta tgctacgtgc
     4801 agtccagagg cctccagaga gcccctgaga agaccaaggg caaagtgagc atgaggttct
     4861 cctctctctg cccaccctcc acgtccactg cctctggccg cagacccagc ctcgtgatcg
     4921 atgggagaag cctggcctac gctctcgaga aaaacctgga ggacaaattc ctcttccttg
     4981 ccaagcagtg ccgctccgtc ctctgctgtc ggtcgacgcc tctgcagaag agcatggtgg
     5041 tgaagctggt gcggagcaag ctcaaggcca tgaccctggc cataggtgat ggagccaatg
     5101 atgtcagcat gatccaggtg gcagatgtgg gtgtgggaat ctccggccag gagggtatgc
     5161 aggcagtgat ggccagcgac tttgcagtgc cgaaattccg atacctggag aggctcttga
     5221 ttcttcacgg gcattggtgc tactcccgac ttgccaacat ggtgctgtac ttcttctaca
     5281 aaaacacaat gttcgtgggc ctcctgtttt ggttccagtt tttctgtggc ttctctgcat
     5341 ctaccatgat tgaccagtgg tatctaatct tctttaatct gctcttctcg tcacttcccc
     5401 cgctcgtgac tggggtgctg gacagggatg tgccagccaa tgtgctgctg accaacccgc
     5461 agctctacaa gagtggccag aacatggagg aataccggcc acgaacgttc tggtttaaca
     5521 tggccgacgc cgccttccag agcctggttt gcttttccat tccttacctg gcctactatg
     5581 actcgaacgt ggacctgttt acctggggga cccctattgt gacaatcgcg ctgctcactt
     5641 tcctgctcca cctgggcatt gaaaccaaaa cctggacctg gctcaactgg ataacgtgtg
     5701 gcttcagtgt ccttttgttt ttcaccgtgg ctttgattta caatgcgtct tgtgccacgt
     5761 gctatcctcc gtccaaccct tactggacta tgcaagcctt actgggtgac ccagtgtttt
     5821 acttgacttg cctgatgacg cctgtcgctg cactgctgcc cagattgttt ttcagatccc
     5881 tccaggggag ggttttcccc acacaacttc agctggcacg tcagttgacc aggaagtccc
     5941 ccaggagatg cagtgctccc aaagagacct ttgctcaggg acgcctcccg aaggactcgg
     6001 gaaccgagca ctcatcaggg aggacagtca agacctctgt gcccctgtcc cagccttctt
     6061 ggcacacaca gcagccggtc tgctccctgg aggccagcgg ggagcccagc acagtggaca
     6121 tgagcatgcc agtgagggag cacaccctgc tggaggggct gagcgcaccg gcccccatgt
     6181 cctctgcgcc aggggaggct gtcctgagga gtccaggagg gtgtcctgag gagtccaagg
     6241 tgagagctgc cagcaccggc agggtgaccc ccctgtcttc cctcttcagc ctgcctacct
     6301 tcagcttact caactggatt tcctcctggt cgctggtcag caggctgggg agtgtcttac
     6361 agttctcccg gacggagcag cttgcagatg gacaagcggg acgtggactt cctgtccagc
     6421 cccactcagg ccgatcagga cttcaagggc cagaccacag actacttata ggagcatctt
     6481 caaggcggtc acagcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg
     6541 ttgatctaga gggcccgcgg ttcgaactcg agtatccata cgatgtacca gactacgcag
     6601 agatccacta gttcaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg
     6661 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat
     6721 tatgtactat ttaaactcga gcggccgcca gcacagtggt cgactgtgga atgtgtgtca
     6781 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6841 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6901 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6961 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7021 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7081 tggaggccta ggcttttgca aaaagcttca cgctgccgca agcactcagg gcgcaagggc
     7141 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     7201 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     7261 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     7321 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     7381 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     7441 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7501 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     7561 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     7621 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     7681 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     7741 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     7801 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7861 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7921 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7981 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8041 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     8101 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     8161 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     8221 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcatcgtcga
     8281 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     8341 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     8401 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
     8461 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
     8521 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     8581 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
     8641 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     8701 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     8761 ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     8821 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     8881 tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt
     8941 tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa
     9001 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9061 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9901 acctagatcc ttttgcggcc ggccgcaaat caatctaaag tatatatgag taaacttggt
     9961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10081 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10261 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10561 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10921 taggggttcc gcgcacattt c
//
