LOCUS       sequence-41330-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG7b
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    404..525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(415..434)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     469..488
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        658..762
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
       61 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc
      121 tataccggtc tcgatatcac tactagtgtt ctagatacaa ctaaatgtgg caaagcttct
      181 tcattgtttg aataatgaaa aacgaacact accaactttg aaatcaaaac attaaaactt
      241 acaacatttc gttcaataaa gtatcatcaa aagaagaaaa caaaagctga acatgagaat
      301 ttgggataag gagcagcaga tcggaattat gtgagaagca cgcggaaaac agggatatat
      361 aaacggggta aacgggaaaa tcggccgctt cgagcagaca tgataagata cattgatgag
      421 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      481 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      541 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      601 ctctacaaat gtggtaaaat cgataaggat ccaggtggca cttttcgggg aaatgtgcgc
      661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      721 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      781 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      841 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      901 ctggatctca acagcggta
//
