LOCUS       sequence-413301-        1494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of EcSTH which 
            can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio
            in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Yang R, Yang C, Ma L, Zhao Y, Guo Z, Niu J, Chu Q, Ma Y, Li B
  TITLE     Identification of purine biosynthesis as an NADH-sensing pathway to 
            mediate energy stress.
  JOURNAL   Nat Commun. 2022 Nov 17;13(1):7031. doi: 10.1038/s41467-022-34850-0.
  PUBMED    36396642
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-17"; volume: "13(1):7031. doi"; pages: " 
            10.1038/s41467-022-34850-0"
COMMENT     Sequence Label: EcSTH
FEATURES             Location/Qualifiers
     source          1..1494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
       61 gatgacaagg gaggaggagg atcaggagga ggaatgcccc actcctacga ctacgacgcc
      121 atcgtgatcg gctccggccc cggcggcgag ggcgccgcca tgggcctggt gaagcagggc
      181 gcccgcgtgg ccgtgatcga gcgctaccag aacgtgggcg gcggctgcac ccactggggc
      241 accatcccct ccaaggccct gcgccacgcc gtgtcccgca tcatcgagtt caaccagaac
      301 cccctgtact ccgaccactc ccgcctgctg cgctcctcct tcgccgacat cctgaaccac
      361 gccgacaacg tgatcaacca gcagacccgc atgcgccagg gcttctacga gcgcaaccac
      421 tgcgagatcc tgcagggcaa cgcccgcttc gtggacgagc acaccctggc cctggactgc
      481 cccgacggct ccgtggagac cctgaccgcc gagaagttcg tgatcgcctg cggctcccgc
      541 ccctaccacc ccaccgacgt ggacttcacc cacccccgca tctacgactc cgactccatc
      601 ctgtccatgc accacgagcc ccgccacgtg ctgatctacg gcgccggcgt gatcggctgc
      661 gagtacgcct ccatcttccg cggcatggac gtgaaggtgg acctgatcaa cacccgcgac
      721 cgcctgctgg ccttcctgga ccaggagatg tccgactccc tgtcctacca cttctggaac
      781 tccggcgtgg tgatccgcca caacgaggag tacgagaaga tcgagggctg cgacgacggc
      841 gtgatcatgc acctgaagtc cggcaagaag ctgaaggccg actgcctgct gtacgccaac
      901 ggccgcaccg gcaacaccga ctccctggcc ctgcagaaca tcggcctgga gaccgactcc
      961 cgcggccagc tgaaggtgaa ctccatgtac cagaccgccc agccccacgt gtacgccgtg
     1021 ggcgacgtga tcggctaccc ctccctggcc tccgccgcct acgaccaggg ccgcatcgcc
     1081 gcccaggccc tggtgaaggg cgaggccacc gcccacctga tcgaggacat ccccaccggc
     1141 atctacacca tccccgagat ctcctccgtg ggcaagaccg agcagcagct gaccgccatg
     1201 aaggtgccct acgaggtggg ccgcgcccag ttcaagcacc tggcccgcgc ccagatcgtg
     1261 ggcatgaacg tgggcaccct gaagatcctg ttccaccgcg agaccaagga gatcctgggc
     1321 atccactgct tcggcgagcg cgccgccgag atcatccaca tcggccaggc catcatggag
     1381 cagaagggcg gcggcaacac catcgagtac ttcgtgaaca ccaccttcaa ctaccccacc
     1441 atggccgagg cctaccgcgt ggccgccctg aacggcctga accgcctgtt ctag
//
