LOCUS       sequence-41333-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG8b
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    356..477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(367..386)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     421..440
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
       61 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc
      121 tataccggtc tcgatatcac tactagtgtt ctagattgga aaatgtgatg tttttctata
      181 aataaatatt ctcacaactc tttttcatgt tttatataac actacatgca catcaagcag
      241 aaaaatttca acataaagtt tacaccagaa gtgaatttag ggatgaagag gaaccaaatt
      301 acgtaaacac tacagtatcg aaacatgata gatcggccgc ttcgagcaga catgataaga
      361 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      421 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
      481 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
      541 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg cccccgaaga
      901 acgttttcca atgatgagca ctttaaagtt ctgctatgtg gcgcggtatt atccc
//
