LOCUS       sequence-413358-       10713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP10D mutant E215Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin HW
  TITLE     Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is
            Involved in Plasma Membrane Dynamics.
  JOURNAL   J Biol Chem. 2015 Jun 12;290(24):15004-17. doi: 
            10.1074/jbc.M115.655191. Epub 2015 May 6.
  PUBMED    25947375
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.655191"; journalName: "J Biol Chem"; date: 
            "2015-06-12- 12"; volume: "290"; issue: "24"; pages: "15004-17"
COMMENT     Sequence Label: pMx/ATP10D(E215Q)-HA
FEATURES             Location/Qualifiers
     source          1..10713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6289..6313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6345..6371
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6540..6560)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6545..6874
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6725..6860
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7231..8025
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7285..7304)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7895..7914
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8218..8687
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8817..8834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8988..9576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9068..9087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9740..10531,10532..10600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10363..10382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10601..10705)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ctctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccaccatg actgaggctc tccaatgggc cagatatcac tggcgacggc
     2041 tgatcagagg tgcaaccagg gatgatgatt cagggccata caactattcc tcgttgctcg
     2101 cctgtgggcg caagtcctct cagatcccta aactgtcagg aaggcaccgg attgttgttc
     2161 cccacatcca gcccttcaag gatgagtatg agaagttctc cggagcctat gtgaacaatc
     2221 gaatacgaac aacaaagtac acacttctga attttgtgcc aagaaattta tttgaacaat
     2281 ttcacagagc tgccaattta tatttcctgt tcctagttgt cctgaactgg gtacctttgg
     2341 tagaagcctt ccaaaaggaa atcaccatgt tgcctctggt ggtggtcctt acaattatcg
     2401 caattaaaga tggcctggaa gattatcgga aatacaaaat tgacaaacag atcaataatt
     2461 taataactaa agtttatagt aggaaagaga aaaaatacat tgaccgatgc tggaaagacg
     2521 ttactgttgg ggactttatt cgcctctcct gcaacgaggt catccctgca gacatggtac
     2581 tactcttttc cactgatcca gatggaatct gtcacattga gacttctggt cttgatggac
     2641 agagcaattt aaaacagagg caggtggttc ggggatatgc agaacaggac tctgaagttg
     2701 atcctgagaa gttttccagt aggatagaat gtgaaagccc aaacaatgac ctcagcagat
     2761 tccgaggctt cctagaacat tccaacaaag aacgcgtggg tctcagtaaa gaaaatttgt
     2821 tgcttagagg atgcaccatt agaaacacag aggctgttgt gggcattgtg gtttatgcag
     2881 gccatgaaac caaagcaatg ctgaacaaca gtgggccacg gtataagcgc agcaaattag
     2941 aaagaagagc aaacacagat gtcctctggt gtgtcatgct tctggtcata atgtgcttaa
     3001 ctggcgcagt aggtcatgga atctggctga gcaggtatga aaagatgcat tttttcaatg
     3061 ttcccgagcc tgatggacat atcatatcac cactgttggc aggattttat atgttttgga
     3121 ccatgatcat tttgttacag gtcttgattc ctatttctct ctatgtttcc atcgaaattg
     3181 tgaagcttgg acaaatatat ttcattcaaa gtgatgtgga tttctacaat gaaaaaatgg
     3241 attctattgt tcagtgccga gccctgaaca tcgccgagga tctgggacag attcagtacc
     3301 tcttttccga taagacagga accctcactg agaataagat ggtttttcga agatgtagtg
     3361 tggcaggatt tgattactgc catgaagaaa atgccaggag gttggagtcc tatcaggaag
     3421 ctgtctctga agatgaagat tttatagaca cagtcagtgg ttccctcagc aatatggcaa
     3481 aaccgagagc ccccagctgc aggacagttc ataatgggcc tttgggaaat aagccctcaa
     3541 atcatcttgc tgggagctct tttactctag gaagtggaga aggagccagt gaagtgcctc
     3601 attccagaca ggctgctttc agtagcccca ttgaaacaga cgtggtacca gacaccaggc
     3661 ttttagacaa atttagtcag attacacctc ggctctttat gccactagat gagaccatcc
     3721 aaaatccacc aatggaaact ttgtacatta tcgacttttt cattgcattg gcaatttgca
     3781 acacagtagt ggtttctgct cctaaccaac cccgacaaaa gatcagacac ccttcactgg
     3841 gggggttgcc cattaagtct ttggaagaga ttaaaagtct tttccagaga tggtctgtcc
     3901 gaagatcaag ttctccatcg cttaacagtg ggaaagagcc atcttctgga gttccaaacg
     3961 cctttgtgag cagactccct ctctttagtc gaatgaaacc agcttcacct gtggaggaag
     4021 aggtctccca ggtgtgtgag agcccccagt gctccagtag ctcagcttgc tgcacagaaa
     4081 cagagaaaca acacggtgat gcaggcctcc tgaatggcaa ggcagagtcc ctccctggac
     4141 agccattggc ctgcaacctg tgttatgagg ccgagagccc agacgaagcg gccttagtgt
     4201 atgccgccag ggcttaccaa tgcactttac ggtctcggac accagagcag gtcatggtgg
     4261 actttgctgc tttgggacca ttaacatttc aactcctaca catcctgccc tttgactcag
     4321 taagaaaaag aatgtctgtt gtggtccgac accctctttc caatcaagtt gtggtgtata
     4381 cgaaaggcgc tgattctgtg atcatggagt tactgtcggt ggcttcccca gatggagcaa
     4441 gtctggagaa acaacagatg atagtaaggg agaaaaccca gaagcacttg gatgactatg
     4501 ccaaacaagg ccttcgtact ttatgtatag caaagaaggt catgagtgac actgaatatg
     4561 cagagtggct gaggaatcat tttttagctg aaaccagcat tgacaacagg gaagaattac
     4621 tacttgaatc tgccatgagg ttggagaaca aacttacatt acttggtgct actggcattg
     4681 aagaccgtct gcaggaggga gtccctgaat ctatagaagc tcttcacaaa gcgggcatca
     4741 agatctggat gctgacaggg gacaagcagg agacagctgt caacatagct tatgcatgca
     4801 aactactgga gccagatgac aagcttttta tcctcaatac ccaaagtaaa gatgcctgtg
     4861 ggatgctgat gagcacaatt ttgaaagaac ttcagaagaa aactcaagcc ctgccagagc
     4921 aagtgtcatt aagtgaagat ttacttcagc ctcctgtccc ccgggactca gggttacgag
     4981 ctggactcat tatcactggg aagaccctgg agtttgccct gcaagaaagt ctgcaaaagc
     5041 agttcctgga actgacatct tggtgtcaag ctgtggtctg ctgccgagcc acaccgctgc
     5101 agaaaagtga agtggtgaaa ttggtccgca gccatctcca ggtgatgacc cttgctattg
     5161 gtgatggtgc caatgatgtt agcatgatac aagtggcaga cattgggata ggggtctcag
     5221 gtcaagaagg catgcaggct gtgatggcca gtgactttgc cgtttctcag ttcaaacatc
     5281 tcagcaagct ccttcttgtc catggacact ggtgttatac acggctttcc aacatgattc
     5341 tctatttttt ctataagaat gtggcctatg tgaacctcct tttctggtac cagttctttt
     5401 gtggattttc aggaacatcc atgactgatt actgggtttt gatcttcttc aacctcctct
     5461 tcacatctgc ccctcctgtc atttatggtg ttttggagaa agatgtgtct gcagagaccc
     5521 tcatgcaact gcctgaactt tacagaagtg gtcagaaatc agaggcatac ttaccccata
     5581 ccttctggat caccttattg gatgcttttt atcaaagcct ggtctgcttc tttgtgcctt
     5641 attttaccta ccagggctca gatactgaca tctttgcatt tggaaacccc ctgaacacag
     5701 ccgctctgtt catcattctc ctccatctgg tcattgaaag caagagtttg acttggattc
     5761 acttgctggt catcattggt agcatcttgt cttatttttt atttgccata gtttttggag
     5821 ccatgtgtgt aacttgcaac ccaccatcca acccttactg gattatgcag gagcacatgc
     5881 tggatccagt attctactta gtttgtatcc tcacgacgtc cattgctctt ctgcccaggt
     5941 ttgtatacag agttcttcag ggatccctgt ttccatctcc aattctgaga gctaagcact
     6001 ttgacagact aactccagag gagaggacta aagctctcaa gaagtggaga ggggctggaa
     6061 agatgaatca agtgacatca aagtatgcta accaatcagc tggcaagtca ggaagaagac
     6121 ccatgcctgg cccttctgct gtatttgcaa tgaagtcagc aagttcctgt gctattgagc
     6181 aaggaaactt atctctgtgt gaaactgctt tagatcaagg ctactctgaa actaaggcct
     6241 ttgagatggc tggaccctcc aaaggtaaag aaagctcgag atatctagac ccagctttct
     6301 tgtacaaagt ggttgatcta gagggcccgc ggttcgaact cgagtatcca tacgatgtac
     6361 cagactacgc agagatccac tagttcaatt ccagctgagc gccggtcgct accattacca
     6421 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
     6481 aaggaaatcc attatgtact atttaaactc gagcggccgc cagcacagtg gtcgactgtg
     6541 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6601 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6661 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6721 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6841 aggaggcttt tttggaggcc taggcttttg caaaaagctt cacgctgccg caagcactca
     6901 gggcgcaagg gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc
     6961 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
     7021 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
     7081 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     7141 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct
     7201 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     7261 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7321 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7381 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     7441 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7501 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7561 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7621 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7681 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7741 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7801 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7861 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7921 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7981 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     8041 tcgcatcgtc gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     8101 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     8161 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     8221 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     8281 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     8341 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     8401 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     8461 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     8521 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     8581 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     8641 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     8701 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     8761 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8821 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8881 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8941 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9001 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9061 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9421 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9661 aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg
     9721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10681 aaaataaaca aataggggtt ccgcgcacat ttc
//
