LOCUS       sequence-413371-        9692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP11C isoform c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Inoue H, Takatsu H, Hamamoto A, Takayama M, Nakabuchi R, Muranaka Y,
            Yagi T, Nakayama K, Shin HW
  TITLE     The interaction of ATP11C-b with ezrin contributes to its polarized 
            localization.
  JOURNAL   J Cell Sci. 2021 Oct 15;134(20):jcs258523. doi: 10.1242/jcs.258523. 
            Epub 2021 Oct 18.
  PUBMED    34528675
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-15"; volume: "134(20):jcs258523. doi"; pages: 
            " 10.1242/jcs.258523. Epub 2021 Oct 18"
COMMENT     Sequence Label: pMx/ATP11C-c-HA
FEATURES             Location/Qualifiers
     source          1..9692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5073..5084)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     protein_bind    complement(5398..5422)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5454..5480
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5519..5539)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5524..5853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5704..5839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6210..7004
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6264..6283)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6874..6893
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7197..7666
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7796..7813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7967..8555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8047..8066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8719..9510,9511..9579))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9342..9361
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9580..9684)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccaccatg cagatggtcc catctctccc tccagcctct gagtgtgctg
     2041 gagaagagaa acgagttggc acacgcacag tgtttgttgg caatcatcca gtttcggaaa
     2101 cagaagctta cattgcacaa agattttgtg ataatagaat agtctcatct aagtatacac
     2161 tttggaattt tctcccaaag aatctgtttg aacagtttag aagaattgca aatttttatt
     2221 ttctcataat cttccttgta caggtcacag tagacacacc aactagccca gttaccagtg
     2281 gacttccact tttctttgtt ataactgtta cagccatcaa gcagggatat gaggattggc
     2341 tgagacacag agctgacaat gaagtcaaca aaagcactgt ttacattatt gaaaatgcaa
     2401 agcgagtgag aaaagaaagt gaaaaaatca aggttggtga tgtagtagaa gtacaggcag
     2461 atgaaacctt tccctgtgat cttattcttc tatcatcttg caccactgat ggaacctgtt
     2521 atgtcactac agccagtctt gatggggaat ccaattgcaa gacacattat gcagtacgtg
     2581 ataccattgc actgtgtaca gcagaatcca tcgataccct ccgagcagca attgaatgtg
     2641 aacagcctca acctgacctc tacaaatttg ttgggcgaat caatatctac agtaatagtc
     2701 ttgaggctgt tgccaggtct ttgggacctg aaaatctctt gctgaaagga gctacgctaa
     2761 aaaataccga gaagatatat ggagttgctg tttacactgg aatggaaacc aaaatggctt
     2821 tgaactacca agggaaatct cagaaacgtt ctgctgttga aaaatctatt aatgctttcc
     2881 tgattgtata tttatttatc ttactgacca aagctgcagt atgcactact ctaaagtatg
     2941 tttggcaaag taccccatac aatgatgaac cttggtataa ccaaaagact cagaaagagc
     3001 gagagacctt gaaggtttta aaaatgttca ccgacttcct atcatttatg gttctattca
     3061 actttatcat tcctgtctcc atgtacgtca cagtagaaat gcagaaattc ttgggctcct
     3121 tcttcatctc atgggataag gacttttatg atgaagaaat taatgaagga gccctggtta
     3181 acacatcaga ccttaatgaa gaacttggtc aggtggatta tgtatttaca gataagactg
     3241 gaacactcac tgaaaacagc atggaattca ttgaatgctg catagatggc cacaaatata
     3301 aaggtgtaac tcaagaggtt gatggattat ctcaaactga tggaacttta acatattttg
     3361 acaaagtaga taagaatcga gaagagctgt ttctacgtgc cttgtgttta tgtcatactg
     3421 tagaaatcaa aacaaacgat gctgttgatg gagctacaga atcagctgaa ttaacctata
     3481 tctcctcttc accagatgaa atagctttgg tgaaaggagc taaaaggtac gggttcacat
     3541 ttttaggaaa tcgaaatgga tatatgagag tagagaacca aagaaaagaa atagaagaat
     3601 atgaacttct tcacacctta aactttgatg ctgtccggcg acgtatgagt gtaattgtga
     3661 agactcaaga aggagacata cttctctttt gtaaaggagc agactcggca gtttttccca
     3721 gagtgcaaaa tcatgaaatt gagttaacta aagtccatgt ggaacgtaat gcaatggatg
     3781 ggtatcggac actctgtgta gccttcaaag aaattgctcc agatgattat gaaagaatta
     3841 acagacagct catagaggca aaaatggcct tacaagacag agaagaaaaa atggaaaaag
     3901 ttttcgatga tattgagaca aacatgaatt taattggagc cactgcagtt gaagacaagc
     3961 tacaagatca agctgcagag accattgaag ctctgcatgc agcaggcctg aaagtctggg
     4021 tgctcactgg ggacaagatg gagacagcta aatccacatg ctatgcctgc cgccttttcc
     4081 agaccaacac tgagctctta gaactaacca caaaaaccat tgaagaaagt gaaaggaaag
     4141 aagatcgatt acatgaatta ttgatagaat atcgcaagaa attgctgcat gagtttccta
     4201 aaagtactag aagctttaaa aaagcatgga cagaacatca ggaatatgga ttaatcatag
     4261 atggctccac attgtcactc atactaaatt ctagtcaaga ctctagttca aacaattaca
     4321 aaagcatttt cctacaaata tgtatgaagt gtactgcagt gctctgctgt cggatggcac
     4381 cattacagaa agcccagatt gtcagaatgg tgaagaattt aaaaggcagc ccaataactc
     4441 tgtcgatagg tgatggtgcc aatgatgtta gtatgatctt ggaatcccat gtgggaatag
     4501 gtattaaagg caaagaaggt cgccaagcag ctaggaatag cgattattct gttccaaagt
     4561 ttaaacactt aaagaaactg ctgttggctc atggacatct atattatgtg agaatagcac
     4621 accttgtaca gtacttcttc tataagaacc tttgtttcat tttgccacag tttttgtacc
     4681 agttcttctg tggattctca caacagccac tgtatgatgc tgcttacctt acaatgtaca
     4741 atatctgctt cacatccttg cccatcctgg cctatagtct actggaacag cacatcaaca
     4801 ttgacactct gacctcagat ccccgattgt atatgaaaat ttctggcaat gccatgctac
     4861 agttgggccc cttcttatat tggacatttc tggctgcctt tgaagggaca gtgttcttct
     4921 ttgggactta ctttcttttt cagactgcat ccctagaaga aaatggaaag gtatacggaa
     4981 actggacttt tggaaccatt gtttttacag tcttagtatt cactgtaacc ctgaagcttg
     5041 ccttggatac ccgattctgg acgtggataa atcactttgt gatttggggt tctttagcct
     5101 tctatgtatt tttctcattc ttctggggag gaattatttg gccttttctc aagcaacaga
     5161 gaatgtattt tgtatttgcc caaatgctgt cttctgtatc cacatggttg gctataattc
     5221 ttctaatatt tatcagcctg ttccctgaga ttcttctgat agtattaaag aatgtaagaa
     5281 gaagaagtgc cagggtaacg aaacgcctcc cttcctcagg aacatctgct atcttcatgc
     5341 tttctcaaac ttccagcaat cacagtttct cttggagtga actctcgaga tatctagacc
     5401 cagctttctt gtacaaagtg gttgatctag agggcccgcg gttcgaactc gagtatccat
     5461 acgatgtacc agactacgca gagatccact agcggccgcc agcacagtgg tcgactgtgg
     5521 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5581 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5641 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5701 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5761 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     5821 ggaggctttt ttggaggcct aggcttttgc aaaaagcttc acgctgccgc aagcactcag
     5881 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
     5941 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
     6001 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
     6061 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     6121 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg
     6181 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6241 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6301 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6361 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6421 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6481 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6541 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6601 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6661 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6721 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6781 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6841 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6901 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6961 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     7021 cgcatcgtcg acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     7081 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     7141 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
     7201 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     7261 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     7321 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     7381 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     7441 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     7501 gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc
     7561 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     7621 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
     7681 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
     7741 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7801 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7861 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7921 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7981 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8041 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8101 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8161 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8221 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8281 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8341 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8401 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8461 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8521 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8581 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8641 aaaggatctt cacctagatc cttttgcggc cggccgcaaa tcaatctaaa gtatatatga
     8701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9061 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9121 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9181 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9241 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9301 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9361 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9421 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9481 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9541 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9661 aaataaacaa ataggggttc cgcgcacatt tc
//
