LOCUS       sequence-413376-        9285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, EP400_SFSWAP from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_EP400_SFSWAP
FEATURES             Location/Qualifiers
     source          1..9285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(32..227)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      46..181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    286..307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(322..327,328..345,346..352)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    360..376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     365..387
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     384..400
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     384..400
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     743..762
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    761..816
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     862..881
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3380..4090,4091..4093,4094..4096))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3422..3443)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3771..3790
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4030..4051
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4093..4102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4178..4381)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4207..4227)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4382..4685
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(4942..4986)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5171..5404
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5764..5881
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6373..6498
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6545..6802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        6804..7183
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7214..7318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7319..7387,7388..8179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7537..7556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8350..8938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8839..8858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9092..9109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    join(9177..9285,1..26)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9201..9220)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9255..9274
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccattataag ctgcaataaa caagttagct ttttgcaaaa gcctaggcct ccaaaaaagc
       61 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
      121 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
      181 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcgga tccgctggca
      241 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      301 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      361 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
      421 gctgcaggtc gagggatctc cataagagaa gagggacagc tatgactggg agtagtcagg
      481 agaggaggaa aaatctggct agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa
      541 aaaataacac aaaacaaaat ataaaaaaaa tctaacctca agtcaaggct tttctatgga
      601 ataaggaatg gacagcaggg ggctgtttca tatactgatg acctctttat agccaacctt
      661 tgttcatggc agccagcata tgggcatatg ttgccaaact ctaaaccaaa tactcattct
      721 gatgttttaa atgatttgcc ctcccatatg tccttccgag tgagagacac aaaaaattcc
      781 aacacactat tgcaatgaaa ataaatttcc tttattagcc agaagtcaga tgctcaaggg
      841 gcttcatgat gtccccataa tttttggcag agggaaaaag atctgctagc tcgagcagct
      901 gaagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
      961 gatctggtct aaccagagag acccagtaca ggcaaaaagc agatcttgtc ttctttggga
     1021 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
     1081 cttgtaagtc attggtctta aaggtaccgt aatcttatct agaaccggtt caggacgcgg
     1141 aggtttgcag gttcttgctc gcagccagca ttgcgcgaac ttttgccatt aggtcttgag
     1201 tgatctttga ctgaacagag ctaacgatcg cgctggagat ctgtgcctct ttttcttggc
     1261 tcacgcctct tgaacgttcc tgagaactac ctctgctttc aaccgggctg acgctcgcgc
     1321 tgtgcgcgct gtgcgcagcc ggagccgcag ggcgtggcgc tgcctgcttg gacggtgaca
     1381 cggattggct acgtgccttg ctcttggtac gggagcggct acggcgcttc ttcttctttt
     1441 cgtgcgggct tctgctgcgc gtacgtttgc gcgacgcgcc cggagaacgg ctgactcggt
     1501 aagccgttgg gacggaacgc tcttcacgac gacgttccgg gctatgcgca cggcgacggg
     1561 gactccgact acgactcctg ctggttttac ccggtttacc ctcaaagctt gaggcgctgt
     1621 cgaaccgagt cgtggtggac gccgcttggt agctacccgg ttccgccgcc gcggtgacgg
     1681 acgcgctgga ttggcacggt tccgcgcgca gggtacctgg agacgcggtc acgcgggagc
     1741 gacgaaccgt acgatacgcg gacggcaagc tatgtttcgc cttagagtgg ctgcggctac
     1801 ggcttttgga cgaagaatgg tatttcggag agcggctacg ggtgcgagaa cgctttttgt
     1861 gcttcttctt ctttttctcc ttctcctctt cacgcggcgg atctttgctc ttgctggacg
     1921 gacgatccgg aggtttaact tccaaggtcg gcagcgggcg gccaccagtc agcaacgggc
     1981 acggggtggt actgctctct tcgtcgttaa tcacttcaga tttctcctcc tcctcctcct
     2041 cctcctcctc ctcctcttcg atgtccaggt cgttctgacg caggtacgct tgcagcggtg
     2101 cgtagtgttt ctttttaaac gccaggaaat ccatcatgtt accctgaaag tgttgcagga
     2161 aaaacagttc aatcagatat tctttgaaca cctctttcag tgcctgcatc tcttgatagt
     2221 gataatccag acattgttta cgcatttgcg ccatttccgg cgaagccggc ggtatctctt
     2281 ccagcttttt cggcaccttt ttcataccgg tgttaccgac gctggtcagc ggcagggagc
     2341 ttagacccgg cggaactgcc gtgcgggagg ggctcgttgg aggcggcggg ctggtcatac
     2401 cgaagccagc cgcaccacca gcgcccggca gtaagacccc cggcgtacgt tcgaagccga
     2461 gcggacgcgg taatggcgga ccttgcagta cctgctgctg ctgcacaagc tgaccctgga
     2521 tgatcgagct gatctgtggc ggcagattag cggtggtaat atggcttgga gaagccagcg
     2581 gttgcaagcc agcaccacca gccgcggccg ggctctgagg acccagatga tgaatggtgc
     2641 caccactttg ggtgtgaggt tgtgacggac gacgttcgcg aacggtgatg ggggtgcctg
     2701 gatgctgcaa ttgaacctgc gcgccctggg cgatctgggt taaaccgctg ccgtcctgaa
     2761 aaacgaaatg accaccgctg ctcggtgaca ggctgatttg acgaaccaac acggatgcat
     2821 ccacaaatcc gcctgtcggg gatgagctgc aaagaccgag gcccggaccc ggcgcaccgg
     2881 cacgaacgtt ttgcagtgct tggcccggac ccggtgacgg ctgcgtcggg ctttgggtct
     2941 gcacttgttg gctcaacggc gaggtcacct gaatatagga cggggagccg tgctcgaaac
     3001 ggcgaggctg cgcgctgaac tggaagcccg gagaagtcgg gctcggcaac ggcagcgggg
     3061 ccaacgtaat ctgctggttg cctccaacaa ccgggcccac actctgcagg gtaatattca
     3121 cgttctggcc ggtagccggg ctacgattca tcagttgttg aatctggtag ctcgggctct
     3181 gcggcgcaga cgggctagca gacggtgcaa atggagccgc cggcgatggc ggcggattcg
     3241 ggtgggccgg ctgctcttca ccttcgctgc ccggacatgc acgagaacgt tggagctggt
     3301 gctgtacatt ttggggtcct gtcccgtgat gcattccccg ggaagcggcc gcacttcccc
     3361 cggagcctcc gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     3421 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
     3481 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     3541 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     3601 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     3661 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     3721 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     3781 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     3841 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     3901 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     3961 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     4021 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     4081 cgcccttgct caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
     4141 gaattcggtt cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc
     4201 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     4261 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     4321 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     4381 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     4441 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     4501 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     4561 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     4621 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     4681 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     4741 aactagcacg cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa
     4801 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     4861 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     4921 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     4981 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     5041 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     5101 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     5161 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     5221 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     5281 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     5341 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     5401 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     5461 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     5521 cctcatatct cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg
     5581 tctacttgcc acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc
     5641 ttttcttctt ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc
     5701 tttccagagg agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac
     5761 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     5821 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     5881 attgtggatg aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac
     5941 ctgtcctata attttcttta attctttatt catagattct actactcctt gactttgggg
     6001 attgtaggga atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt
     6061 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     6121 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     6181 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     6241 gccagtatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     6301 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     6361 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     6421 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     6481 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     6541 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     6601 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     6661 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     6721 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     6781 agtcccgccc aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc
     6841 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     6901 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     6961 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     7021 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     7081 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     7141 cccgtaattg attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt
     7201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7501 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7561 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7861 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8041 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8161 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8461 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9061 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcggatc
     9181 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     9241 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaa
//
