LOCUS       sequence-413383-        9986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses ATP10B with the catalytic mutation D433N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Martin S, Smolders S, Van den Haute C, Heeman B, van Veen S, 
            Crosiers D, Beletchi I, Verstraeten A, Gossye H, Gelders G, Pals P, 
            Hamouda NN, Engelborghs S, Martin JJ, Eggermont J, De Deyn PP, Cras 
            P, Baekelandt V, Vangheluwe P, Van Broeckhoven C
  TITLE     Mutated ATP10B increases Parkinson's disease risk by compromising 
            lysosomal glucosylceramide export.
  JOURNAL   Acta Neuropathol. 2020 Jun;139(6):1001-1024. doi: 
            10.1007/s00401-020-02145-7. Epub 2020 Mar 14.
  PUBMED    32172343
REFERENCE   2  (bases 1 to 9986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00401-020-02145-7"; journalName: "Acta Neuropathol"; date:
            "2020-06"; volume: "139"; issue: "6"; pages: "1001-1024"
COMMENT     Sequence Label: pcDNA3_ATP10B-D433N
FEATURES             Location/Qualifiers
     source          1..9986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1063..1087
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2140..2151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5507..5531)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5539..5557)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5540..5557)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5577..5594)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5583..5807
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5853..6281
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5940..5959)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6150..6171
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6290..6310)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6295..6624
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6475..6610
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6537..6556
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6691..7485
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6745..6764)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7355..7374
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7659..7780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7696..7715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7750..7769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7829..7845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7829..7845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7842..7864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7853..7869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7877..7883,7884..7901,7902..7907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7922..7943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8060..8077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8231..8819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8311..8330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8990..9781,9782..9850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9613..9632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9851..9955)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 ccggggaatt cggcagatcc cgaggtccga cagcgcccgg cccagatccc cacgcctgcc
      961 aggagcaagc cgagagccag ccggccggcg cactccgact ccgagcagtc tctgtccttc
     1021 gacccgagcc ccgcgccctt tccgggaccc ctgccccatc aaacaagttt gtacaaaaaa
     1081 gcaggcttga aggaattcgg taccatggcc ctctcagtgg actcatcgtg gcatcggtgg
     1141 cagtggagag tcagagatgg cttcccccat tgtccatcgg aaaccacacc gctgctctct
     1201 ccagagaaag ggagacagag ctacaacttg acacagcagc gggtcgtgtt ccccaacaac
     1261 agcatattcc atcaagattg ggaagaggtc tccaggagat accctggcaa cagaacctgc
     1321 acaaccaaat acaccctctt caccttcctg ccccggaatc tctttgagca atttcataga
     1381 tgggctaacc tctatttcct gttcctggtg attttgaact ggatgccctc catggaagtc
     1441 ttccacagag aaatcaccat gttaccattg gccattgtcc tgttcgtcat catgatcaag
     1501 gatggcatgg aggacttcaa gagacaccgc tttgataaag caataaactg ctccaacatt
     1561 cgaatttatg aaagaaaaga gcagacctat gtgcagaagt gctggaagga tgtgcgcgtg
     1621 ggagacttca tccaaatgaa atgcaatgag attgtcccag cagacatact cctccttttt
     1681 tcctctgacc ccaatgggat atgccatctg gaaactgcca gcttggatgg agagacaaac
     1741 ctcaagcaaa gatgtgtcgt gaagggcttc tcacagcagg aggtacagtt cgaaccagag
     1801 cttttccaca ataccatcgt gtgtgagaaa cccaacaacc acctcaacaa atttaagggt
     1861 tatatggagc atcctgacca gaccaggact ggctttggct gtgagagtct tctgcttcga
     1921 ggctgcacca tcagaaacac cgagatggct gttggcattg tcatctatgc aggccatgag
     1981 acgaaagcca tgctgaacaa cagtggcccc cggtacaaac gcagcaagat tgagcggcgc
     2041 atgaatatag acatcttctt ctgcattggg atcctcatcc tcatgtgcct tattggagct
     2101 gtaggtcaca gcatctggaa tgggaccttt gaagaacacc ctcccttcga tgtgccagat
     2161 gccaatggca gcttccttcc cagtgccctt gggggcttct acatgttcct cacaatgatc
     2221 atcctgctcc aggtgctgat ccccatctct ttgtatgtct ccattgagct ggtgaagctc
     2281 gggcaagtgt tcttcttgag caatgacctt gacctgtatg atgaagagac cgatttatcc
     2341 attcaatgtc gagccctcaa catcgcagag gacttgggcc agatccagta catcttctcc
     2401 aataagacgg ggaccctgac agagaacaag atggtgttcc gacgttgcac catcatgggc
     2461 agcgagtatt ctcaccaaga aaatgctaag cgactggaga ccccaaagga gctggactca
     2521 gatggtgaag agtggaccca ataccaatgc ctgtccttct cggctagatg ggcccaggat
     2581 ccagcaacta tgagaagcca aaaaggtgct cagcctctga ggaggagcca gagtgcccgg
     2641 gtgcccatcc agggccacta ccggcaaagg tctatggggc accgtgaaag ctcacagcct
     2701 cctgtggcct tcagcagctc catagaaaaa gatgtaactc cagataaaaa cctactgacc
     2761 aaggttcgag atgctgccct gtggttggag accttgtcag acagcagacc tgccaaggct
     2821 tccctctcca ccacctcctc cattgctgat ttcttccttg ccttaaccat ctgcaactct
     2881 gtcatggtgt ccacaaccac cgagcccagg cagagggtca ccatcaaacc ctcaagcaag
     2941 gctctgggga cgtccctgga gaagattcag cagctcttcc agaagttgaa gctattgagc
     3001 ctcagccagt cattctcatc cactgcaccc tctgacacag acctcgggga gagcttaggg
     3061 gccaacgtgg ccaccacaga ctcggatgag agagatgatg catctgtgtg cagtggaggt
     3121 gactccactg atgacggtgg ctacaggagc agcatgtggg accagggcga catcctggag
     3181 tctgggtcag gcacttcctt ggaggaggca ttggaggccc cagccacaga cctggccagg
     3241 cctgagttct gttacgaggc tgagagccct gatgaggccg ccctggtgca cgctgcccat
     3301 gcctacagct tcacactagt gtcccggaca cctgagcagg tgactgtgcg cctgccccag
     3361 ggcacctgcc tcaccttcag cctcctctgc accctgggct ttgactctgt caggaagaga
     3421 atgtctgtgg ttgtgaggca cccactgact ggcgagattg ttgtctacac caagggtgct
     3481 gactcggtca tcatggacct gctggaagac ccagcctgcg tacctgacat taatatggaa
     3541 aagaagctga gaaaaatccg agcccggacc caaaagcatc tagacttgta tgcaagagat
     3601 ggcctgcgca cactatgcat tgccaagaag gttgtaagcg aagaggactt ccggagatgg
     3661 gccagtttcc ggcgtgaggc tgaggcatcc ctcgacaacc gagatgagct tctcatggaa
     3721 actgcacagc atctggagaa tcaactcacc ttacttggag ccactgggat cgaagaccgg
     3781 ctgcaggaag gagttccaga tacgattgcc actctgcggg aggctgggat ccagctctgg
     3841 gtcctgactg gagataagca ggagacagcg gtcaacattg cccattcctg cagactgtta
     3901 aatcagaccg acactgttta taccatcaat acagagaatc aggagacctg tgaatccatc
     3961 ctcaattgtg cattggaaga gctaaagcaa tttcgtgaac tacagaagcc agaccgcaag
     4021 ctctttggat tccgcttacc ttccaagaca ccatccatca cctcagaagc tgtggttcca
     4081 gaagctggat tggtcatcga tgggaagaca ttgaatgcca tcttccaggg aaagctagag
     4141 aagaagtttc tggaattgac ccagtattgt cggtccgtcc tgtgctgccg ctccacgcca
     4201 ctccagaaga gtatgatagt caagctggtg cgagacaagt tgcgcgtcat gaccctttcc
     4261 ataggtgatg gagcaaatga tgtaagcatg attcaagctg ctgatattgg aattggaata
     4321 tctggacagg aaggcatgca ggctgtcatg tccagcgact ttgccatcac ccgctttaag
     4381 catctcaaga agttgctgct cgtgcatggc cactggtgtt actcgcgcct ggccaggatg
     4441 gtggtgtact acctctacaa gaacgtgtgc tacgtcaacc tgctcttctg gtatcagttc
     4501 ttctgtggtt tctccagctc caccatgatt gattactggc agatgatatt cttcaatctc
     4561 ttctttacct ccttgcctcc tcttgtcttt ggagtccttg acaaagacat ctctgcagaa
     4621 acactcctgg cattgcctga gctatacaag agtggccaga actctgagtg ctataacctg
     4681 tcgactttct ggatttctat ggtggatgca ttctaccaga gcctcatctg tttctttatc
     4741 ccttacctgg cctataaggg ctctgatata gatgtcttta cctttgggac accaatcaac
     4801 accatctccc tcaccacaat ccttttgcac caggcaatgg aaatgaagac atggaccatt
     4861 ttccacggag tcgtgctcct cggcagcttc ctgatgtact ttctggtatc cctcctgtac
     4921 aatgccacct gcgtcatctg caacagcccc accaatccct attgggtgat ggaaggccag
     4981 ctctcaaacc ccactttcta cctcgtctgc tttctcacac cagttgttgc tcttctccca
     5041 agatactttt tcctgtctct gcaaggaact tgtgggaagt ctctaatctc aaaagctcag
     5101 aaaattgaca aactcccccc agacaaaaga aacctggaaa tccagagttg gagaagcaga
     5161 cagaggcctg cccctgtccc cgaagtggct cgaccaactc accacccagt gtcatctatc
     5221 acaggacagg acttcagtgc cagcacccca aagagctcta accctcccaa gaggaagcat
     5281 gtggaagagt cagtactcca cgaacagaga tgtggcacgg agtgcatgag ggatgactca
     5341 tgctcagggg actcctcagc tcaactctca tccggggagc acctgctggg acctaacagg
     5401 ataatggcct actcaagagg acagactgat atgtgccggt gctcaaagag gagcagccat
     5461 cgccgatccc agagttcact gaccatatag ctcgagtgcg gccgcaaccc agctttcttg
     5521 tacaaagtgg ttcgatattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     5581 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5641 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5701 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5761 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5821 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5881 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5941 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6001 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6061 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6121 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6181 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6241 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6301 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6361 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6421 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6481 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6541 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6601 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6661 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6721 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6781 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6841 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6901 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6961 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7021 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7081 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7141 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7201 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7261 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7321 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7381 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7441 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7501 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     7561 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7621 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7681 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7741 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7801 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7861 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7921 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7981 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9121 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9181 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9241 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9301 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9361 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9421 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9481 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9541 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9601 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9661 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9721 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9781 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9841 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9901 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9961 ttccccgaaa agtgccacct gacgtc
//
