LOCUS       sequence-413386-        9360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP9A mutant E195Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Tone T, Nakayama K, Takatsu H, Shin HW
  TITLE     ATPase reaction cycle of P4-ATPases affects their transport from the
            endoplasmic reticulum.
  JOURNAL   FEBS Lett. 2020 Feb;594(3):412-423. doi: 10.1002/1873-3468.13629. 
            Epub 2019 Oct 14.
  PUBMED    31571211
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.13629"; journalName: "FEBS Lett"; date: 
            "2020-02"; volume: "594"; issue: "3"; pages: "412-423"
COMMENT     Sequence Label: pCAG/ATP9A(E195Q)-HA
FEATURES             Location/Qualifiers
     source          1..9360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1988..3004
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2928..2950
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3012..3031
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3100..3124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6314..6338)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6382..6408
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6496..6515)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6561..6616
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6615..6634)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6977..6993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6977..6993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6990..7012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7001..7017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7025..7031,7032..7049,7050..7055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7070..7091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7150..7345
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7196..7331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7351..7485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7388..7407)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7442..7461
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7553..7570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7724..8312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7804..7823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8483..9274,9275..9343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9106..9125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9344..9360,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg ggcgcccggg gggggggggg gggggggggg
     1801 gggggggggg gggggggggg gggggggggg gggggcgcgg gggggggggg gggggggggg
     1861 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     1921 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     1981 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     2041 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     2101 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     2161 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     2221 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     2281 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     2341 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     2401 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     2461 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     2521 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     2581 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     2641 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     2701 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     2761 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     2821 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     2881 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     2941 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     3001 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaatttgc
     3061 tagcgagctc gcggccgcag atctctagtt aagctatcaa caagtttgta caaaaaagca
     3121 ggctctttaa aggaaccaat tcagtcgact ggatccacca tgacggacaa catcccgctg
     3181 cagccggtgc gccagaagaa gcggatggac agcaggcccc gcgccgggtg ctgcgagtgg
     3241 ctgagatgct gcggtggagg ggaggccagg ccccgcactg tctggctggg gcaccccgag
     3301 aagagagacc agaggtatcc tcggaatgtc atcaacaatc agaagtacaa tttcttcacc
     3361 tttcttcctg gggtgctgtt caaccagttc aaatactttt tcaacctcta tttcttactt
     3421 cttgcctgct ctcagtttgt tcccgaaatg agacttggtg cactctatac ctactgggtt
     3481 cccctgggct tcgtgctggc cgtcactgtc atccgtgagg cggtggagga gatccgatgc
     3541 tacgtgcggg acaaggaagt caactcccag gtctacagcc ggctcacagc acgaggcaca
     3601 gtgaaggtga agagttctaa catccaagtt ggagacctta tcatcgttga aaagaaccag
     3661 cgggtccctg ccgacatgat cttcctgagg acatcagaaa aaaacgggtc atgcttcttg
     3721 cggacggatc agctggatgg gcagacggac tggaagctgc ggcttcccgt ggcctgcacg
     3781 cagaggctcc ccacggccgc cgaccttctt cagattcgat cgtatgtgta cgcagaagag
     3841 ccaaatattg acattcacaa cttcgtggga acttttaccc gagaagacag cgaccccccg
     3901 atcagcgaga gcctgagcat agagaacacg ctgtgggctg gcactgtggt cgcatcaggt
     3961 actgttgtgg gtgttgttct ttacactggc agagaactcc ggagtgtcat gaatacctca
     4021 aatcctcgaa gtaagatcgg cctgttcgac ttggaagtga actgcctcac caagatcctc
     4081 tttggtgccc tggtggtggt ctcgctggtc atggttgccc ttcagcactt tgcaggccgt
     4141 tggtacctgc agatcatccg cttcctcctc ttgttttcca acatcatccc cattagtttg
     4201 cgcgtgaacc tggacatggg caagatcgtg tacagctggg tgattcgaag ggactcaaaa
     4261 atccccggga ccgtggttcg ctccagcacg attcctgagc agctgggcag gatttcgtac
     4321 ttactcacag acaagacagg cactcttacc cagaacgaga tgattttcaa acggctccat
     4381 ctcggaacag tagcctacgg cctcgactca atggacgaag tacaaagcca catttttagc
     4441 atttacaccc agcaatccca ggacccaccg gctcagaagg gcccaacgct caccactaag
     4501 gtccggcgga ccatgagcag ccgcgtgcac gaagccgtga aggccatcgc gctctgccac
     4561 aacgtgactc ccgtgtatga gtccaacggt gtgactgatc aggctgaggc cgagaagcag
     4621 tacgaagact cctgccgcgt ataccaggca tccagccccg atgaggtggc cctggtacag
     4681 tggacggaaa gtgtgggctt aaccctggtg ggccgagacc agtcttccat gcagctgagg
     4741 acccctggcg accagatcct gaacttcacc atcctacaga tcttcccttt cacctatgaa
     4801 agcaaacgta tgggcatcat cgtgcgggat gaatcaactg gagaaattac gttttacatg
     4861 aagggagcag atgtggtcat ggctggcatt gtgcagtaca atgactggtt ggaggaagag
     4921 tgtggcaaca tggcccgaga agggctgcgg gtgctcgtgg tggcaaagaa gtctcttgca
     4981 gaggagcagt atcaggactt tgaagcccgc tacgtccagg ccaagctgag tgtgcacgac
     5041 cgctccctca aagtggccac ggtgatcgag agcctggaga tggagatgga actgctgtgc
     5101 ctgacgggcg tggaggacca gctgcaggca gatgtgcggc ccacgctgga gaccctgagg
     5161 aatgctggca tcaaggtttg gatgctgaca ggggacaagc tggagacagc tacgtgcaca
     5221 gcgaagaatg cacatctggt gaccagaaac caagacatcc acgtttttcg gctggtgacc
     5281 aaccgcgggg aggctcacct cgagctgaac gccttccgca ggaagcatga ttgtgccctg
     5341 gtcatctcgg gagactccct ggaggtttgc ctcaagtact atgagtacga gttcatggag
     5401 ctggcctgcc agtgcccggc cgtagtctgc tgccgatgtg cccccaccca gaaggcccag
     5461 atcgtgcgcc tgcttcagga gcgcacgggc aagctcacct gtgcagtagg ggacggaggc
     5521 aatgacgtca gcatgattca ggaatctgac tgcggcgtgg gagtggaagg aaaggaagga
     5581 aaacaggctt cgttggctgc agacttctcc atcactcaat ttaagcatct tggccggttg
     5641 cttatggtgc atggccggaa cagctacaag cggtcagccg ccctcagcca gttcgtgatt
     5701 cacaggagcc tctgtatcag caccatgcag gctgtctttt cctccgtgtt ttactttgcc
     5761 tccgtccctc tctatcaagg attcctcatc attgggtact ccacaattta caccatgttt
     5821 cctgtgtttt ctctggtcct ggacaaagat gtcaaatcgg aagttgccat gctgtatcct
     5881 gagctctaca aggatcttct caagggacgg ccgttgtcct acaagacatt cttaatatgg
     5941 gttttgatta gcatctatca agggagcacc atcatgtacg gggcgctgct gctgtttgag
     6001 tcggagttcg tgcacatcgt ggccatctcc ttcacctcgc tgatcctcac cgagctgctc
     6061 atggtggcgc tgaccatcca gacctggcac tggctcatga cagtggcgga gctgctcagc
     6121 ctggcctgct acatcgcctc cctggtgttc ttacacgagt tcatcgatgt gtacttcatc
     6181 gccaccttgt cattcttgtg gaaagtctcc gtcatcactc tggtcagctg cctccccctc
     6241 tatgtcctca agtacctgcg aagacggttc tctcccccca gctactcaaa gctcacatcg
     6301 tcgagatatc tagacccagc tttcttgtac aaagtggttg atctagaggg cccgcggttc
     6361 gaactcgagg taccgaattc gtacccatac gacgtcccag actacgctta attcactcct
     6421 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
     6481 taccactgag atcgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     6541 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     6601 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     6661 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     6721 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     6781 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     6841 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     6901 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     6961 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7021 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7081 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7141 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7201 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7261 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7321 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     7381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     7501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8161 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
//
