LOCUS       sequence-413388-        9588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP9B mutant E272Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Tone T, Nakayama K, Takatsu H, Shin HW
  TITLE     ATPase reaction cycle of P4-ATPases affects their transport from the
            endoplasmic reticulum.
  JOURNAL   FEBS Lett. 2020 Feb;594(3):412-423. doi: 10.1002/1873-3468.13629. 
            Epub 2019 Oct 14.
  PUBMED    31571211
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.13629"; journalName: "FEBS Lett"; date: 
            "2020-02"; volume: "594"; issue: "3"; pages: "412-423"
COMMENT     Sequence Label: pCAG/ATP9B(E272Q)-HA
FEATURES             Location/Qualifiers
     source          1..9588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1949..2965
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2889..2911
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2973..2992
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3061..3085
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6542..6566)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6610..6636
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6724..6743)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6789..6844
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6843..6862)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7205..7221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7205..7221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7218..7240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7229..7245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7253..7259,7260..7277,7278..7283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7298..7319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7378..7573
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7424..7559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7579..7713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7616..7635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7670..7689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7781..7798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7952..8540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8032..8051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8711..9502,9503..9571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9334..9353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9572..9588,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg cgggccgggg gggggggggg ggggcggggg
     1801 gggggggggg gggggggggg gggggggggg gggggcgagg cggagaggtg cggcggcagc
     1861 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1921 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1981 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     2041 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     2101 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     2161 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     2221 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     2281 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     2341 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     2401 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     2461 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     2521 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     2581 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2641 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     2701 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     2761 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     2821 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     2881 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     2941 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     3001 tcattttggc aaagaatttg ctagcgagct cgcggccgca gatctctagt taagctatca
     3061 acaagtttgt acaaaaaagc aggctcttta aaggaaccaa ttcagtcgac tggatccacc
     3121 atggcggacc agatcccgct ttacccggtg cgtagcgcag cggcggccgc agccaaccgc
     3181 aaacgcgcgg cctactacag cgccgcgggg cccaggccgg gagccgaccg gcacagcagg
     3241 taccagctgg aggatgagtc tgcgcatttg gatgaaatgc cactaatgat gtctgaagaa
     3301 ggctttgaga atgaggaaag tgattaccac accttaccac gagccaggat aatgcaaagg
     3361 aaaagaggac tggagtggtt tgtctgtgat ggctggaagt tcctctgtac cagttgctgt
     3421 ggttggctga taaatatttg tcgaagaaag aaagagctga aagctcgcac agtatggctt
     3481 ggatgtcctg aaaagtgtga agaaaaacat cccaggaatt ctataaaaaa tcaaaaatac
     3541 aatgtgttta cctttatacc tggggttttg tatgaacaat tcaagttttt cttgaatctc
     3601 tattttctag taatatcctg ctcacagttt gtaccagcat tgaaaatagg ctatctctac
     3661 acctactggg ctcctctggg atttgtcttg gctgttacta tgacacggga agcaattgat
     3721 gaatttcggc gttttcagcg tgacaaggaa gtgaattcac aactatatag caagcttaca
     3781 gtaagaggta aagtgcaagt taagagttca gacatacaag ttggagacct catcatagtg
     3841 gaaaagaatc aaagaattcc atcggacatg gtgtttctta ggacttcaga aaaagcaggt
     3901 tcgtgtttta ttcgaactga tcaactagat ggtcaaactg actggaagct gaaggtggca
     3961 gtgagctgca cgcaacagct gccggctctg ggggaccttt tttctatcag tgcttatgtt
     4021 tatgctcaga aaccacaaat ggacattcac agtttcgaag gcacatttac cagggaagac
     4081 agtgacccgc ccattcatga aagtctcagc atagaaaata cattgtgggc aagcaccatt
     4141 gttgcatcag gtactgtaat aggtgttgtc atttataccg gaaaagagac tcgaagtgta
     4201 atgaacacat ccaatccaaa aaataaggtt ggtttgttgg accttgaact caatcggctg
     4261 acgaaagcgc tatttttggc tttagttgct ctttccattg ttatggtaac cttacaagga
     4321 tttgtgggtc catggtaccg caatcttttt cggttccttc tcctcttttc ttacatcatt
     4381 cccataagtt tgcgtgtgaa cttggacatg ggcaaagcgg tgtatggatg gatgatgatg
     4441 aaagatgaga acatccctgg cacggtcgtt cggaccagca ctatcccaga ggaacttggg
     4501 cgcctggtgt atttattgac agacaaaaca ggaaccctca cccagaatga aatgatattt
     4561 aagcggctgc acctgggcac cgtgtcctat ggcgccgaca cgatggatga gatccagagc
     4621 catgtcaggg actcctactc acagatgcag tctcaagctg gtggaaacaa tactggttca
     4681 actccactaa gaaaagccca atcttcagct cccaaagtta ggaaaagtgt cagtagtcga
     4741 atccatgaag ccgtgaaagc catcgtgctg tgtcacaacg tgacccccgt gtatgagtct
     4801 cgggccggcg ttactgagga gactgagttc gcagaggctg accaagactt cagtgatgag
     4861 aatcgcacct accaggcttc cagcccggat gaggtcgctc tggtgcagtg gacagagagt
     4921 gtgggcctca cgctggtcag cagggacctc acctccatgc agctgaagac ccccagtggc
     4981 caggtcctca gcttctgcat tctgcagctg tttcccttca cctccgagag caagcggatg
     5041 ggcgtcatcg tcagggatga atccacggca gaaatcacat tctacatgaa gggcgctgac
     5101 gtggccatgt ctcctatcgt gcagtataat gactggctgg aagaggagtg cggaaacatg
     5161 gctcgcgaag gactgcggac cctcgtggtt gcaaagaagg cgttgacaga ggagcagtac
     5221 caggactttg agagccgata cactcaagcc aagctgagca tgcacgacag gtccctcaag
     5281 gtggccgcgg tagtcgagag cctggagagg gagatggaac tgctgtgcct caccggcgtg
     5341 gaggaccagc tgcaggcaga cgtgcggccc acgctggaga tgctgcgcaa cgccgggatc
     5401 aagatatgga tgctaacagg cgataaactc gagacagcta cctgcattgc caaaagttca
     5461 catctcgtgt ctagaacaca agatattcat attttcagac aggtaaccag tcggggagag
     5521 gcacatttgg agctgaatgc atttcgaagg aagcatgatt gtgcactagt catatctggg
     5581 gactctctgg aggtttgtct aaagtactac gagcatgaat ttgtggagct ggcctgccag
     5641 tgccctgccg tggtttgctg ccgctgctca cccacccaga aggcccgcat tgtgacactg
     5701 ctgcagcagc acacagggag acgcacctgc gccatcggtg atggaggaaa tgatgtcagc
     5761 atgattcagg cagcagactg tgggattggg attgagggaa aggagggtaa gcaggcctcg
     5821 ctggcggccg acttctccat cacgcagttc cggcacatag gcaggctgct catggtgcac
     5881 gggcggaaca gctacaagag gtcggcggca ctcggccagt tcgtcatgca caggggcctt
     5941 atcatctcca ccatgcaggc tgtgttttcc tcagtcttct acttcgcatc cgtccctttg
     6001 tatcagggct tcctcatggt ggggtatgcc accatataca ccatgttccc agtgttctcc
     6061 ttagtgctgg accaggacgt gaagccagag atggcgatgc tctacccgga gctgtacaag
     6121 gacctcacca agggaagatc cttgtccttc aaaaccttcc tcatctgggt tttaataagt
     6181 atttaccaag gcggcatcct catgtatggg gccctggtgc tcttcgagtc tgagttcgtc
     6241 cacgtggtgg ccatctcctt caccgcactg atcctgaccg agctgctgat ggtggcgctg
     6301 accgtccgca cgtggcactg gctgatggtg gtggccgagt tcctcagctt aggctgctac
     6361 gtgtcctcac tcgcttttct caatgaatat tttgatgttg cctttatcac caccgtgacc
     6421 ttcctgtgga aagtgtcggc gatcaccgtg gtcagctgcc tcccgctgta tgtcctcaag
     6481 tacctgaggc gcaagctctc tcctcccagc tactgcaagc tggcctcgtc gagatatcta
     6541 gacccagctt tcttgtacaa agtggttgat ctagagggcc cgcggttcga actcgaggta
     6601 ccgaattcgt acccatacga cgtcccagac tacgcttaat tcactcctca ggtgcaggct
     6661 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     6721 cgatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     6781 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     6841 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     6901 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     6961 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     7021 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     7081 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     7141 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     7201 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7261 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7321 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     7381 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7501 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7561 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7621 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7681 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     7741 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaat
//
