LOCUS       sequence-41339-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG10b
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    419..540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(430..449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     484..503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        673..777
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
       61 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc
      121 tataccggtc tcgatatcac tactagtgtt ctagacttca caaaaagtgt ggtgcgcgca
      181 ttccacgggc tacgaacaca tgggtaaact gtacattttc aaatattgtt gaaaactttt
      241 aatttttcaa ttttaaattc aaactttatg ttttaagcaa taaaatgatg atttaatccg
      301 ttacactacg acatggaaaa gttacagtta gttttttttt ttaagcggtc gttatttata
      361 ggggttcgtt taatggtgtc acatactgct ttgcgtcggc cgcttcgagc agacatgata
      421 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      481 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      541 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      601 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccagg tggcactttt
      661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      721 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      781 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      841 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtg gt
//
