LOCUS       sequence-41342-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG11b
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    264..385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(275..294)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     329..348
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        518..622
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(840..859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
       61 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc
      121 tataccggtc tcgatatcac tactagtgtt ctagattgag cctttatatt gtatttgaat
      181 gagctttgag tattataatg attatctctc ttggaaacgt ttttgcacta cataaacaaa
      241 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
      301 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      361 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      421 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
      481 cgataaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      541 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      601 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      661 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      721 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      781 gatccttgag agttttcgcc ccgaagaacg ttttccatga tgagcacttt taaagttctg
      841 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgt cgcg
//
