LOCUS       sequence-41344-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG12a
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    333..454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(344..363)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     398..417
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        587..691
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctgc atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 ctataccggt ctcgatatca ctactagtgt tctagagcaa cgattaaata tagattctac
      181 ctctctgttt catttcatgt gcgatagttt cagataatta tttattttat attttgtatt
      241 ttatgaacgg gttcgatact tgtctttttt cggttggaat gtacaaaaat acacagaata
      301 cacgaattga cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
      361 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      421 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      481 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      541 tggtaaaatc gataaggatc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      841 cagcggta
//
