LOCUS       sequence-41345-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG12b
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    332..453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(343..362)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     397..416
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        586..690
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
       61 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc
      121 tataccggtc tcgatatcac tactagtgtt ctagagcaac gattaaatat agattctacc
      181 tctctgtttc atttcatgtg cgatagtttc agataattat ttattttata ttttgtattt
      241 tatgaacggg ttcgatactt gtcttttttc ggttggaatg cactacaata cacagaatac
      301 acgaattgac ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
      361 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      421 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
      481 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      541 ggtaaaatcg ataaggatcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      661 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      721 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      781 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      841 agcggtaaga tcc
//
