LOCUS       sequence-41347-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG13a
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    263..384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(274..293)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     328..347
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        517..621
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(841..860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctgc atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 ctataccggt ctcgatatca ctactagtgt tctagaagtt ttttgatgta caaaactagc
      181 caattttttg tatcagatct tttattgatt gtttacgttt gaacggttcc atttgccaaa
      241 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
      301 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      361 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      421 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
      481 gataaggatc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacctt ttaaagttct
      841 gctatgtggc gcggtattat cccgtaattg acgcccgggc caagaagcaa ctcggtcgcc
      901 gcatacacta tttctcagaa a
//
