LOCUS       sequence-413479-        9689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP11B mutant E180Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Tone T, Nakayama K, Takatsu H, Shin HW
  TITLE     ATPase reaction cycle of P4-ATPases affects their transport from the
            endoplasmic reticulum.
  JOURNAL   FEBS Lett. 2020 Feb;594(3):412-423. doi: 10.1002/1873-3468.13629. 
            Epub 2019 Oct 14.
  PUBMED    31571211
REFERENCE   2  (bases 1 to 9689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.13629"; journalName: "FEBS Lett"; date: 
            "2020-02"; volume: "594"; issue: "3"; pages: "412-423"
COMMENT     Sequence Label: pCAG/ATP11B(E180Q)-HA
FEATURES             Location/Qualifiers
     source          1..9689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1927..2943
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2867..2889
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2951..2970
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3039..3063
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6643..6667)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6711..6737
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6825..6844)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6890..6945
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6944..6963)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7306..7322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7306..7322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7319..7341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7330..7346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7354..7360,7361..7378,7379..7384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7399..7420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7479..7674
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7525..7660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7680..7814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7717..7736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7771..7790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7882..7899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8053..8641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8133..8152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8812..9603,9604..9672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9435..9454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9673..9689,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg gcgcgcccgg gggggggggg gggggggggg
     1801 gggggggggg gggggggggg gggggggggg gggggggccc aggggggggg ggggggcccc
     1861 agggttcttt ttttggcggg gggggggggg gggggccccc ataaaaaacg aagcgcgcgg
     1921 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     1981 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     2041 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     2101 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     2161 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     2221 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     2281 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     2341 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     2401 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     2461 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     2521 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
     2581 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     2641 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     2701 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     2761 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     2821 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     2881 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     2941 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaatttgct
     3001 agcgagctcg cggccgcaga tctctagtta agctatcaac aagtttgtac aaaaaagcag
     3061 gctctttaaa ggaaccaatt cagtcgactg gatctaccat gtggcgctgg atccggcagc
     3121 agctgggttt tgacccacca catcagagtg acacaagaac catctacgta gccaacaggt
     3181 ttcctcagaa tggcctttac acacctcaga aatttataga taacaggatc atttcatcta
     3241 agtacactgt gtggaatttt gttccaaaaa atttatttga acagttcaga agagtggcaa
     3301 acttttattt tcttattata tttttggttc agcttatgat tgatacacct accagtccag
     3361 ttaccagtgg acttccatta ttctttgtga taacagtaac tgccataaag cagggatatg
     3421 aagattggtt acggcataac tcagataatg aagtaaatgg agctcctgtt tatgttgttc
     3481 gaagtggtgg ccttgtaaaa actagatcaa aaaacattcg ggtgggtgat attgttcgaa
     3541 tagccaaaga tgaaattttt cctgcagact tggtgcttct gtcctcagat cgactggatg
     3601 gttcctgtca cgttacaact gctagtttgg acggacaaac taacctgaag acacatgtgg
     3661 cagttccaga aacagcatta ttacaaacag ttgccaattt ggacactcta gtagctgtaa
     3721 tagaatgcca gcaaccagaa gcagacttat acagattcat gggacgaatg atcataaccc
     3781 aacaaatgga agaaattgta agacctctgg ggccggagag tctcctgctt cgtggagcca
     3841 gattaaaaaa cacaaaagaa atttttggtg ttgcggtata cactggaatg gaaactaaga
     3901 tggcattaaa ttacaagagc aaatcacaga aacgatctgc agtagaaaag tcaatgaata
     3961 catttttgat aatttatcta gtaattctta tatctgaagc tgtcatcagc actatcttga
     4021 agtatacatg gcaagctgaa gaaaaatggg atgaaccttg gtataaccaa aaaacagaac
     4081 atcaaagaaa tagcagtaag attctgagat ttatttcaga cttccttgct tttttggttc
     4141 tctacaattt catcattcca atttcattat atgtgacagt cgaaatgcag aaatttcttg
     4201 gatcgttttt tattggctgg gatcttgatc tgtatcatga agaatcagat cagaaagctc
     4261 aagtcaatac ttccgatctg aatgaagagc ttggacaggt agagtacgtg tttacagata
     4321 aaactggtac actgacagaa aatgagatgc agtttcggga atgttcaatt aatggcatga
     4381 aataccaaga aattaatggt agacttgtac ccgaaggacc aacaccagac tcttcagaag
     4441 gaaacttatc ttatcttagt agtttatccc atcttaacaa cttatcccat cttacaacca
     4501 gttcctcttt cagaaccagt cctgaaaatg aaactgaact aattaaagaa catgatctct
     4561 tctttaaagc agtcagtctc tgtcacactg tacagattag caatgttcaa actgactgca
     4621 ctggtgatgg tccctggcaa tccaacctgg caccatcgca gttggagtac tatgcatctt
     4681 caccagatga aaaggctcta gtagaagctg cagcaaggat tggtattgtg tttattggca
     4741 attctgaaga aactatggag gttaaaactc ttggaaaact ggaacggtac aaactgcttc
     4801 atattctgga atttgattca gatcgtagga gaatgagtgt aattgttcag gcaccttcag
     4861 gtgagaagtt attatttgct aaaggagctg agtcatcaat tctccctaaa tgtataggtg
     4921 gagaaataga aaaaaccaga attcatgtag atgaatttgc tttgaaaggg ctaagaactc
     4981 tgtgtatagc atatagaaaa tttacatcaa aagagtatga ggaaatagat aaacgcatat
     5041 ttgaagccag gactgccttg cagcagcggg aagagaaatt ggcagctgtt ttccagttca
     5101 tagagaaaga cctgatatta cttggagcca cagcagtaga agacagacta caagataaag
     5161 ttcgagaaac tattgaagca ttgagaatgg ctggtatcaa agtatgggta cttactgggg
     5221 ataaacatga aacagctgtt agtgtgagtt tatcatgtgg ccattttcat agaaccatga
     5281 acatccttga acttataaac cagaaatcag acagcgagtg tgctgaacaa ttgaggcagc
     5341 ttgccagaag aattacagag gatcatgtga ttcagcatgg gctggtagtg gatgggacca
     5401 gcctatctct tgcactcagg gagcatgaaa aactatttat ggaagtttgc agaaattgtt
     5461 cagctgtatt atgctgtcgt atggctccac tgcagaaagc aaaagtaata agactaataa
     5521 aaatatcacc tgagaaacct ataacattgg ctgttggtga tggtgctaat gacgtaagca
     5581 tgatacaaga agcccatgtt ggcataggaa tcatgggtaa agaaggaaga caggctgcaa
     5641 gaaacagtga ctatgcaata gccagattta agttcctctc caaattgctt tttgttcatg
     5701 gtcattttta ttatattaga atagctaccc ttgtacagta ttttttttat aagaatgtgt
     5761 gctttatcac accccagttt ttatatcagt tctactgttt gttttctcag caaacattgt
     5821 atgacagcgt gtacctgact ttatacaata tttgttttac ttccctacct attctgatat
     5881 atagtctttt ggaacagcat gtagaccctc atgtgttaca aaataagccc accctttatc
     5941 gagacattag taaaaaccgc ctcttaagta ttaaaacatt tctttattgg accatcctgg
     6001 gcttcagtca tgcctttatt ttcttttttg gatcctattt actaataggg aaagatacat
     6061 ctctgcttgg aaatggccag atgtttggaa actggacatt tggcactttg gtcttcacag
     6121 tcatggttat tacagtcaca gtaaagatgg ctctggaaac tcatttttgg acttggatca
     6181 accatctcgt tacctgggga tctattatat tttattttgt attttccttg ttttatggag
     6241 ggattctctg gccatttttg ggctcccaga atatgtattt tgtgtttatt cagctcctgt
     6301 caagtggttc tgcttggttt gccataatcc tcatggttgt tacatgtcta tttcttgata
     6361 tcataaagaa ggtctttgac cgacacctcc accctacaag tactgaaaag gcacagctta
     6421 ctgaaacaaa tgcaggtatc aagtgcttgg actccatgtg ctgtttcccg gaaggagaag
     6481 cagcgtgtgc atctgttgga agaatgctgg aacgagttat aggaagatgt agtccaaccc
     6541 acatcagcag atcatggagt gcatcggatc ctttctatac caacgacagg agcatcttga
     6601 ctctctccac aatggactca tctacttgct cgagatatct agacccagct ttcttgtaca
     6661 aagtggttga tctagagggc ccgcggttcg aactcgaggt accgaattcg tacccatacg
     6721 acgtcccaga ctacgcttaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     6781 ctggtgtggc caatgccctg gctcacaaat accactgaga tcgatctttt tccctctgcc
     6841 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     6901 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     6961 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     7021 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     7081 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     7141 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     7201 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     7261 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     7321 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7381 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7441 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     7501 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7561 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7621 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     7681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7801 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7861 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7921 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8461 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8701 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8761 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8821 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8881 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8941 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9001 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9061 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9121 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9181 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9241 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9301 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9361 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9421 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9481 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9541 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9601 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9661 ttgaatactc atactcttcc tttttcaat
//
