LOCUS       sequence-41349-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG13c
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    262..383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(273..292)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     327..346
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        516..620
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
       61 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctaggagctc
      121 tataccggtc tcgatatcac tactagtgtt ctagaagttt tttgatgtac gatactagcc
      181 aattttttgt atcagatctt ttattgattg tttacgtttg aacggttcca tttgccaaac
      241 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      301 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      361 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      421 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
      481 ataaggatcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      541 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      601 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      661 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      721 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      781 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      841 tatgtgggcg cggtattatc cc
//
