LOCUS       sequence-413556-       10711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 8 Tn10 backbone part, with RFP dropout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Alker AT, Farrell MV, Demko AM, Purdy TN, Adak S, Moore BS, Sneed 
            JM, Paul VJ, Shikuma NJ
  TITLE     Linking bacterial tetrabromopyrrole biosynthesis to coral 
            metamorphosis.
  JOURNAL   ISME Commun. 2023 Sep 19;3(1):98. doi: 10.1038/s43705-023-00309-6.
  PUBMED    37726481
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ISME 
            Commun."; date: "2023-09-19"; volume: "3(1):98. doi"; pages: " 
            10.1038/s43705-023-00309-6"
COMMENT     Sequence Label: pMMK801
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     525..544
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(564..641)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(863..868,869..884,885..891)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    899..915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     904..926
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3440..3458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3465..3483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             3501..3512
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3519..4196
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4214..4285
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4301..4328
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4385..5200)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4385..5200)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5108..5127
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5192..5211
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6523..6911
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(7529..7900)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7933..8042
                     /note="incP origin of transfer"
     primer_bind     complement(8723..8742)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8881..8903)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9044..9063
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(9120..9911,9912..9980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9743..9762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9981..10085)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(10192..10711,1..563))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
       61 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
      121 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
      181 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
      241 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
      301 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
      361 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
      421 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
      481 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
      541 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
      601 catgccatac cgcgaaaggt tttgcaccat tcgatggtgt cgggtaccga gctctcccgg
      661 gaattctcat gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca
      721 ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt
      781 cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg
      841 gcaaatattc tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt
      901 gtgagcggat aacaatttca cacaggaaac aggatcaaat ggtttcgcga aaaatcaata
      961 atcagacaac aagatgtgcg aactcgatat tttacacgac tctctttacc aattctgccc
     1021 cgaattacac ttaaaacgac tcaacagctt aacgttggct tgccacgcat tacttgactg
     1081 taaaactctc actcttaccg aacttggccg taacctgcca accaaagcga gaacaaaaca
     1141 taacatcaaa cgaatcgacc gattgttagg taatcgtcac ctccacaaag agcgactcgc
     1201 tgtataccgt tggcatgcta gctttatctg ttcgggcaat acgatgccca ttgtacttgt
     1261 tgactggtct gatattcgtg agcaaaaacg acttatggta ttgcgagctt cagtcgcact
     1321 acacggtcgt tctgttactc tttatgagaa agcgttcccg ctttcagagc aatgttcaaa
     1381 gaaagctcat gaccaatttc tagccgacct tgcgagcatt ctaccgagta acaccacacc
     1441 gctcattgtc agtgatgctg gctttaaagt gccatggtat aaatccgttg agaagctggg
     1501 ttggtactgg ttaagtcgag taagaggaaa agtacaatat gcagacctag gagcggaaaa
     1561 ctggaaacct atcagcaact tacatgatat gtcatctagt cactcaaaga ctttaggcta
     1621 taagaggctg actaaaagca atccaatctc atgccaaatt ctattgtata aatctcgctc
     1681 taaaggccga aaaaatcagc gctcgacacg gactcattgt caccacccgt cacctaaaat
     1741 ctactcagcg tcggcaaagg agccatgggt tctagcaact aacttacctg ttgaaattcg
     1801 aacacccaaa caacttgtta atatctattc gaagcgaatg cagattgaag aaaccttccg
     1861 agacttgaaa agtcctgcct acggactagg cctacgccat agccgaacga gcagctcaga
     1921 gcgttttgat atcatgctgc taatcgccct gatgcttcaa ctaacatgtt ggcttgcggg
     1981 cgttcatgct cagaaacaag gttgggacaa gcacttccag gctaacacag tcagaaatcg
     2041 aaacgtactc tcaacagttc gcttaggcat ggaagttttg cggcattctg gctacacaat
     2101 aacaagggaa gacttactcg tggctgcaac cctactagct caaaatttat tcacacatgg
     2161 ttacgctttg gggaaattat gaggggatct ctcagtgcat tgcctccaat tcccataatt
     2221 tattacgccg ataataactt ggtgtaacct taaaaatgta cttaaatcga cgtgtaaaag
     2281 attgttggga atcaaattga tattttaatg cgatctcaag gatagttttt ttcgtcaacc
     2341 tcaactcaac agccgctttc gtcaaacgac gagcacgaat atagctagcc agtgtgaccc
     2401 ctgttacttt ttgaacagcc gctgaaaata ccacttggta taacccgctt tattcgccac
     2461 atcatcaagc agtaaagact gatctaaatt atgttcaatc cataatagaa catctttgat
     2521 aaccgttgta tgaaactgct tatcatcata tcttaattgg atgttattag ctttattttg
     2581 atagcgagaa tgctgttcaa tatacataaa ataacctaaa tgttcttaag attgtcacga
     2641 ccacatcatc atgataccat aaacatactg acggtatgtt attttaaatc tatcatggaa
     2701 aataaaaatc atcaacaaga aaattttaag agtacctatc aatcactggt taactcagca
     2761 cgaatattgt ttgttgaaaa aggctatcaa gctgtttcaa tagatgagat cgatctcggg
     2821 aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt aaaaataaaa aacaattact
     2881 cagtgcctgt tataagcagc aattaattat gattgatgcc tacatcacaa caaaaactga
     2941 tttaacaaat ggttggtctg ccttagaaag tatatttgaa cattatcttg attatattat
     3001 tgataataat aaaaacctta tccctatcca agaagtgatg cctatcattg gttggaatga
     3061 acttgaaaaa attagccttg aatacattac tggtaaggta aacgccattg tcagcaaatt
     3121 gatccaagag aaccaactta aagctttcct gacggaatgt taattctcgt tgaccctgag
     3181 cactgatgaa tcccctaatg attttggtaa aaatcattaa gttaaggtgg atacacatct
     3241 tgtcatatga tcccggatcc ggctgtaatc cgggcagcgc aacggaacat tcatcagtgt
     3301 aaaaatggaa tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtgcggc
     3361 cgcgagcacc tggtcgcttt cagaaatcaa tctaaagtat atatgagtaa acttggtctg
     3421 acaggcccct tgagaccagt ccctatcagt gatagagatt gacatcccta tcagtgatag
     3481 agatactgag cacggatctg aaagaggaga aaggatctat ggcgagtagc gaagacgtta
     3541 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
     3601 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac tcagaccgct aaactgaaag
     3661 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     3721 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     3781 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
     3841 cccaggactc ctcactgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacta
     3901 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     3961 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     4021 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     4081 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
     4141 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataag
     4201 gatctccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     4261 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     4321 tttctgcgtt tataagtcgg tctcaccgag cggccgcgtg ttacaaccaa ttaaccaatt
     4381 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     4441 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     4501 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     4561 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     4621 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     4681 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     4741 attgcgcctg agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     4801 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     4861 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     4921 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     4981 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     5041 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     5101 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     5161 gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     5221 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     5281 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
     5341 aggatcagtc atgaccaaaa tcccttaacg tgagtcagcc tgccgccttg ggccgggtga
     5401 tgtcgtactt gcccggcggc cgcggcctag gcggccacgc gtattcaggc tgaccctgcg
     5461 cgctgcgcag ggctttattg attccatttt tacactgatg aatgttccgt tgcgctgccc
     5521 ggattacagc cggatccggg atcatatgac aagatgtgta tccaccttaa cttaatgatt
     5581 tttaccaaaa tcattagggg attcatcagt gctcagggtc aacgagaatt aacattccgt
     5641 caggaaagct tatgatgatg atgtgcttaa aaacttactc aatggctggt ttatgcatat
     5701 cgcaatacat gcgaaaaacc taaaagagct tgccgataaa aaaggccaat ttattgctat
     5761 ttaccgcggc tttttattga gcttgaaaga taaataaaat agataggttt tatttgaagc
     5821 taaatcttct ttatcgtaaa aaatgccctc ttgggttatc aagagggtca ttatatttcg
     5881 cggaataaca tcatttggtg acgaaataac taagcacttg tctcctgttt actcccctga
     5941 gcttgagggg ttaacatgaa ggtcatcgat agcaggataa taatacagta aaacgctaaa
     6001 ccaataatcc aaatccagcc atcccaaatt ggtagtgaat gattataaat aacagcaaac
     6061 agtaatgggc caataacacc ggttgcattg gtaaggctca ccaataatcc ctgtaaagca
     6121 ccttgctgat gactctttgt ttggatagac atcactccct gtaatgcagg taaagcgatc
     6181 ccaccaccag ccaataaaat taaaacaggg aaaactaacc aaccttcaga tataaacgct
     6241 aaaaaggcaa atgcactact atctgcaata aatccgagca gtactgccgt tttttcgccc
     6301 catttagtgg ctattcttcc tgccacaaag gcttggaata ctgagtgtaa aagaccaaga
     6361 cccgctaatg aaaagccaac catcatgcta ttccatccaa aacgattttc ggtaaatagc
     6421 acccacaccg ttgcgggaat ttggcctatc aattgcgctg aaaaataaat aatcaacaaa
     6481 atgggcatcg ttttaaataa agtgatgtat accgaattcc catgtcagcc gttaagtgtt
     6541 cctgtgtcac tcaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct
     6601 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt
     6661 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt
     6721 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc
     6781 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat
     6841 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgctg
     6901 atcttcagat cctctacgcc ggacgcatcg tggccggatc cagccgacca ggctttccac
     6961 gcccgcgtgc cgctccatgt cgttcgcgcg gttctcggaa acgcgctgcc gcgtttcgtg
     7021 attgtcacgc tcaagcccgt agtcccgttc gagcgtcgcg cagaggtcag cgagggcgcg
     7081 gtaggcccga tacggctcat ggatggtgtt tcgggtcggg tgaatcttgt tgatggcgat
     7141 atggatgtgc aggttgtcgg tgtcgtgatg cacggcactg acgcgctgat gctcggcgaa
     7201 gccaagccca gcgcagatgc ggtcctcaat cgcgcgcaac gtctccgcgt cgggcttctc
     7261 tcccgcgcgg aagctaacca gcaggtgata ggtcttgtcg gcctcggaac gggtgttgcc
     7321 gtgctgggtc gccatcacct cggccatgac agcgggcagg gtgtttgcct cgcagttcgt
     7381 gacgcgcacg tgacccaggc gctcggtctt gccttgctcg tcggtgatgt acttcaccag
     7441 ctccgcgaag tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac
     7501 cccccggccg ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca
     7561 tcatgacctt gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg
     7621 tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc
     7681 ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga
     7741 cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg
     7801 ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag
     7861 gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
     7921 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
     7981 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
     8041 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
     8101 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
     8161 gttatgcagc ggaaaagcgc tgcttccctg ctgttttgtg gaatatctac cgactggaaa
     8221 caggcaaatg caggaaatta ctgaactgag gggacaggcg agagacgatg ccaaagagct
     8281 acaccgacga gctggccgag tgggttgaat cccgcgcggc caagaagcgc cggcgtgatg
     8341 aggctgcggt tgcgttcctg gcggtgaggg cggatgtcga ggcggcgtta gcgtccggct
     8401 atgcgctcgt caccatttgg gagcacatgc gggaaacggg gaaggtcaag ttctcctacg
     8461 agacgttccg ctcgcacgcc aggcggcaca tcaaggccaa gcccgccgat gtgcccgcac
     8521 cgcaggccaa ggctgcggaa cccgcgccgg cacccaagac gccggagcca cggcggccga
     8581 agcagggggg caaggctgaa aagccggccc ccgctgcggc cccgaccggc ttcaccttca
     8641 acccaacacc ggacaaaaag gatcctctac gccggacgca tcgtggccgg catcaccggc
     8701 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     8761 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     8821 gggggactgt tgggcgccat ctccttgctg cctcgcgcgt ttcggtgatg acggtgaaaa
     8881 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     8941 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     9001 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     9061 gtactgagag tgcaccataa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9121 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9181 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9241 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9301 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9361 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9421 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9481 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9541 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9601 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9661 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9721 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9781 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9841 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9901 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9961 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10021 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10081 ccgcgcacat ttccccgaaa agtgccacct gcagatctgc aggtcggaag cataaagtgt
    10141 aaagcctggg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
    10201 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10261 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
    10321 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
    10381 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
    10441 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
    10501 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
    10561 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
    10621 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
    10681 cagacgcaga cgcgccgaga cagaacttaa t
//
