LOCUS       sequence-413565-        4743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC27A3_STOP
FEATURES             Location/Qualifiers
     source          1..4743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2868..2967)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2984..3003)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2985..3003)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3008..3024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3008..3024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3126..3145)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3137..3946
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3204..3223)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4093..4681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4582..4601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggcg tgtgccagag gaccagggca ccttggaagg agaagtctca
      721 gctggagagg gccgccctgg gcttccgcaa gggaggctct ggcatgtttg ccagcggctg
      781 gaaccagaca gtgccaatcg aggaggcagg atccatggcc gccctgctgc tgctgcctct
      841 gctgctgctg ctgccactgc tgctgctgaa gctgcacctg tggccacagc tgaggtggct
      901 gcctgcagat ctggccttcg ccgtgcgggc cctgtgctgt aagagagccc tgagggcaag
      961 agccctggca gcagcagcag ccgacccaga gggccccgag ggcggctgta gcctggcctg
     1021 gcggctggcc gagctggccc agcagagagc cgcccacacc ttcctgatcc acggctcccg
     1081 gagattttct tacagcgagg cagagaggga gtctaatagg gcagcaagag ccttcctgag
     1141 agccctgggc tgggattggg gccccgacgg cggcgatagc ggcgagggct ccgccggcga
     1201 gggcgagagg gccgcccctg gcgccggcga tgccgccgcc ggcagcggcg ccgagtttgc
     1261 cggcggcgac ggagcagcaa ggggaggagg agcagcagcc cctctgtccc caggcgccac
     1321 cgtggccctg ctgctgccag caggacctga gttcctgtgg ctgtggtttg gactggcaaa
     1381 ggcaggactg aggaccgcct tcgtgccaac agccctgagg aggggccctc tgctgcactg
     1441 cctgcggtct tgtggagcaa gagccctggt gctggcacca gagtttctgg agagcctgga
     1501 gccagacctg cccgccctgc gcgccatggg cctgcacctg tgggcagcag gaccaggaac
     1561 ccaccctgca ggcatctccg atctgctggc agaggtgtct gccgaggtgg acggaccagt
     1621 gcctggatac ctgagctccc cacagtctat caccgataca tgcctgtata tcttcacaag
     1681 cggaaccaca ggactgccaa aggcagcaag aatcagccac ctgaagatcc tgcagtgcca
     1741 gggcttttat cagctgtgcg gcgtgcacca ggaggacgtg atctacctgg ccctgcctct
     1801 gtatcacatg tccggctctc tgctgggcat cgtgggctgt atgggcatcg gcgccaccgt
     1861 ggtgctgaag agcaagttct ccgccggcca gttttgggag gattgccagc agcacagggt
     1921 gacagtgttc cagtacatcg gcgagctgtg ccgctatctg gtgaaccagc cacctagcaa
     1981 ggcagagagg ggacacaagg tgcgcctggc agtgggatcc ggactgaggc cagacacctg
     2041 ggagcggttc gtgcggcggt tcggaccact gcaggtgctg gagacctacg gcctgacaga
     2101 gggcaacgtg gccaccatca attatacagg acagagggga gcagtgggaa gagcaagctg
     2161 gctgtacaag cacatcttcc ccttttccct gatccggtat gatgtgacca caggcgagcc
     2221 aatcagagac ccacagggac actgcatggc cacctcccct ggagagccag gactgctggt
     2281 ggcaccagtg tctcagcaga gccccttcct gggatacgca ggaggacctg agctggccca
     2341 gggcaagctg ctgaaggacg tgtttcggcc aggcgacgtg ttcttcaata caggcgacct
     2401 gctggtgtgc gacgatcagg gcttcctgag gtttcacgac cgcaccggcg atacattcag
     2461 atggaagggc gagaacgtgg ccaccacaga ggtggccgag gtgttcgagg ccctggattt
     2521 tctgcaggag gtgaacgtgt acggcgtgac cgtgccagga cacgagggaa gggcaggcat
     2581 ggccgccctg gtgctgcgcc caccccacgc cctggacctg atgcagctgt acacacacgt
     2641 gtccgagaac ctgcctccat atgccaggcc tcgctttctg cggctgcagg agtctctggc
     2701 caccacagag accttcaagc agcagaaggt gagaatggcc aatgagggct ttgacccttc
     2761 cacactgtct gatccactgt acgtgctgga ccaggcagtg ggagcatacc tgccactgac
     2821 cacagcaagg tatagcgccc tgctggccgg caacctgcgc atctaagacc cagctttctt
     2881 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2941 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     3001 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3061 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3121 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3181 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3241 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3301 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3361 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3421 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3481 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3541 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3601 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3661 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3721 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3781 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3841 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3901 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3961 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     4021 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     4081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4201 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4741 gtt
//
