LOCUS       sequence-413579-        3528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35A3_STOP
FEATURES             Location/Qualifiers
     source          1..3528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1653..1752)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1769..1788)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1770..1788)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1793..1809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1793..1809)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1911..1930)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1922..2731
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1989..2008)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2878..3466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3367..3386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgttcg ccaacctgaa gtacgtgagc ctgggcatcc tggtgtttca
      721 gaccacatcc ctggtgctga ccatgcggta ctctagaaca ctgaaggagg agggaccaag
      781 gtatctgagc tccaccgcag tggtggtggc agagctgctg aagatcatgg cctgcatcct
      841 gctggtgtat aaggactcca agtgttctct gagggccctg aaccgcgtgc tgcacgatga
      901 gatcctgaat aagccaatgg agaccctgaa gctggccatc ccctccggca tctacacact
      961 gcagaacaat ctgctgtatg tggccctgtc taacctggac gccgccacct accaggtgac
     1021 atatcagctg aagatcctga ccacagccct gttttccgtg tctatgctga gcaagaagct
     1081 gggcgtgtac cagtggctgt ccctggtcat cctgatgacc ggagtggcct tcgtgcagtg
     1141 gcctagcgac tcccagctgg attccaagga gctgagcgcc ggctcccagt ttgtgggact
     1201 gatggccgtg ctgaccgcat gcttctctag cggctttgcc ggcgtgtact tcgagaagat
     1261 cctgaaggag acaaagcaga gcgtgtggat ccggaatatc cagctgggct tctttggcag
     1321 catcttcggc ctgatgggcg tgtacatcta tgacggcgag ctggtgagca agaacggctt
     1381 ctttcagggc tataataggc tgacatggat cgtggtggtg ctgcaggccc tgggaggact
     1441 ggtcatcgca gccgtgatca agtacgccga taacatcctg aagggctttg ccacctctct
     1501 gagcatcatc ctgtctacac tgatcagcta tttctggctg caggactttg tgcctacctc
     1561 cgtgttcttt ctgggcgcca tcctggtcat caccgccaca ttcctgtacg gctatgatcc
     1621 caagcctgcc ggcaatccaa caaaggccta agacccagct ttcttgtaca aagttggcat
     1681 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1741 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     1801 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1861 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1921 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1981 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     2041 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2101 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     2161 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     2221 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2281 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2341 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2401 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2461 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2521 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2581 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2641 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2701 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2761 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     2821 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
