LOCUS       sequence-413584-        3807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A46_STOP
FEATURES             Location/Qualifiers
     source          1..3807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1932..2031)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2048..2067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2049..2067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2072..2088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2072..2088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2190..2209)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2201..3010
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2268..2287)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3157..3745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3646..3665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgcacc cccggagacc tgacggcttc gatggactgg gatacagggg
      721 aggagcaaga gacgagcagg gattcggagg agcatttcca gcacggagct tttccaccgg
      781 aagcgacctg ggacactggg tgaccacacc acctgatatc cccggctcca gaaacctgca
      841 ctggggcgag aagtctccac cctacggcgt gcctaccaca agcaccccat atgagggccc
      901 cacagaggag cctttcagct ccggaggagg aggatctgtg cagggacagt ctagcgagca
      961 gctgaatagg ttcgcaggat ttggaatcgg actggcatcc ctgtttacag agaacgtgct
     1021 ggcccaccct tgcatcgtgc tgaggcgcca gtgtcaagtg aattaccacg cccagcacta
     1081 tcacctgacc cctttcacag tgatcaacat catgtactct tttaataaga cccagggacc
     1141 aagggccctg tggaagggaa tgggcagcac cttcatcgtg cagggagtga cactgggagc
     1201 agagggcatc atctccgagt ttacacctct gccacgcgag gtgctgcaca agtggagccc
     1261 aaagcagatc ggcgagcacc tgctgctgaa gtccctgacc tacgtggtgg ccatgccctt
     1321 ctattctgcc agcctgatcg agaccgtgca gagcgagatc atccgggaca atacaggcat
     1381 cctggagtgc gtgaaggagg gcatcggcag agtgatcggc atgggcgtgc cacactctaa
     1441 gcgcctgctg cccctgctga gcctgatctt ccctaccgtg ctgcacggcg tgctgcacta
     1501 catcatctcc tctgtgatcc agaagtttgt gctgctgatc ctgaagagga agacctacaa
     1561 ctcccacctg gccgagtcca catctccagt gcagtctatg ctggacgcct atttccccga
     1621 gctgatcgcc aattttgccg ccagcctgtg ctccgatgtg atcctgtatc ctctggagac
     1681 agtgctgcac aggctgcaca tccagggcac ccgcacaatc atcgacaaca ccgatctggg
     1741 ctacgaggtg ctgccaatca atacacagta tgagggcatg cgggattgta tcaacaccat
     1801 cagacaggag gagggcgtgt tcggctttta caagggcttt ggcgccgtga tcatccagta
     1861 taccctgcac gccgccgtgc tgcagatcac caagatcatc tactccacac tgctgcagaa
     1921 caatatctaa gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1981 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2041 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2101 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2161 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2221 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2281 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2341 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2401 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2461 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2521 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2581 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2641 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2701 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2761 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2821 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2881 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2941 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3001 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3061 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3781 cttttgctgg ccttttgctc acatgtt
//
