LOCUS       sequence-41361-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1051
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(90..123)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(757..790)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atgtccatca ggttcttgcg aacctcatca ctcgttgcat cgaccggtaa tgcaggcaaa
       61 ttttggtgta cggtcagtaa attggacatg aagttcctat actttctaga gaataggaac
      121 ttcagatctg agtccggtag cgccacgtga cgcgtcgctc agggtggggt agccgggaaa
      181 ggagatgtct gtggtggttc aaaaagccag gaaccttgag gctgtcaatt gtgacccact
      241 cccaggggtc tggggtttat agtgctctga ggtggcgggg aattgacagg cacttgcgtc
      301 cacacccagg ccagacaaag gcaagtctaa gattagcctg ctgcaggggg gccgtctcca
      361 ggctgggccc aaggaatggc cgctgcggcg ttcagaggcc agggttggat cactgtgccc
      421 ctgatcaccc ccttgtctgt ctatctgctc cctggagatg gggcggtctc cgggcactaa
      481 tgaggccacc ttgcacccag gaatctggga gcacaaaaga ggcgctggcc tgggacaata
      541 tggagggagg cagctgctaa ttaggggcgg gagagaggca gacgcgagac cccacattat
      601 gtgtgtgggg ggtggagcag ccagcagcag tcctctccct cccctgcttc ttgtcgctgc
      661 tcctggggac tgtcaggctc caggaccttc ttccttcatg agctcgaatt cgaacccctt
      721 cgagcttggc tggacgtaaa ctcctcttca gacctaataa cttcgtatag catacattat
      781 acgaagttat attaagggtt attgaatatg atcggaatta attcttgaag ac
//
