LOCUS       sequence-413613-       14904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi for STK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, 
            Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
  TITLE     Comprehensive split TEV based protein-protein interaction screening 
            reveals TAOK2 as a key modulator of Hippo signalling to limit 
            growth.
  JOURNAL   Cell Signal. 2024 Jan;113:110917. doi: 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
  PUBMED    37813295
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Signal."; date: "2024-01"; volume: "113:110917. doi"; pages: " 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7"
COMMENT     Sequence Label: pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)
FEATURES             Location/Qualifiers
     source          1..14904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..66
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             73..93
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(103..129,130..132,133..2616,2617..2622,2623..2688,
                     2689..2691,2692..4206)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(103..129,130..132,133..2619,2620..2622,2623..4206)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             103..4206
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4219..4239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4273..4467
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             4534..4554
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4561..4614
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4615..5214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4615..4634)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5111..5131
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(5268..5301)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5326..5914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5379..5399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5797..5808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5917..5933)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5918..5934)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6439..6619
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6645..6662)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6651..6875
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6921..7349
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7008..7027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7218..7239
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7358..7378)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7363..7692
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7543..7678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7605..7624
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7740..7787
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7806..8180
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8310..8431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8347..8366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8401..8420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8480..8496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8480..8496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8493..8515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8504..8520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8528..8534,8535..8552,8553..8558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8573..8594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8711..8728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8882..9470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8962..8981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9641..10432,10433..10501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10264..10283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10502..10606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10681..10700)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10872..11251
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11253..11451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11406..11426
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11469..11649
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11696..11821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12314..12547
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12732..12776
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13074..13191
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(13235..13268)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(13279..13354)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(13375..13623)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(13433..13452)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(13603..13623)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        13656..14867
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     14704..14723
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      join(14896..14904,1)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
       61 gacaagatgg cccccaagaa gaagaggaag gtgggccgcg gaatggacaa gaagtactcc
      121 attgggctcg ccatcggcac aaacagcgtc ggctgggccg tcattacgga cgagtacaag
      181 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
      241 ctcattggcg ccctcctgtt cgactccggg gaaaccgccg aagccacgcg gctcaaaaga
      301 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
      361 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
      421 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
      481 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
      541 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
      601 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
      661 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
      721 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
      781 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
      841 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg
      901 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
      961 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg
     1021 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat
     1081 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag
     1141 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga
     1201 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc
     1261 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac
     1321 aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa
     1381 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt
     1441 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact
     1501 cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc
     1561 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag
     1621 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag
     1681 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa
     1741 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa
     1801 gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc
     1861 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc
     1921 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt
     1981 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa
     2041 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg
     2101 atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct taagtccgat
     2161 ggatttgcca accggaactt catgcagttg atccatgatg actctctcac ctttaaggag
     2221 gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat
     2281 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa
     2341 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag
     2401 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag
     2461 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt
     2521 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag
     2581 gaactggaca tcaatcggct ctccgactac gacgtggatg ccatcgtgcc ccagtctttt
     2641 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag
     2701 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg
     2761 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt
     2821 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag
     2881 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat
     2941 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc
     3001 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat
     3061 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct
     3121 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag
     3181 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc
     3241 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac
     3301 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc
     3361 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc
     3421 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg
     3481 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg
     3541 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc
     3601 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga
     3661 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt
     3721 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg
     3781 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa
     3841 gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt
     3901 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac
     3961 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca
     4021 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac
     4081 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca
     4141 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt
     4201 ggagacagca gggctgaccc caagaagaag aggaaggtgg ctagcgatgc taagtcactg
     4261 actgcctggt cccggacact ggtgaccttc aaggatgtgt ttgtggactt caccagggag
     4321 gagtggaagc tgctggacac tgctcagcag atcctgtaca gaaatgtgat gctggagaac
     4381 tataagaacc tggtttcctt gggttatcag cttactaagc cagatgtgat cctccggttg
     4441 gagaagggag aagagccctg gctggtggag agagaaattc accaagagac ccatcctgat
     4501 tcagagactg catttgaaat caaatcatca gttccgaaaa agaaacgcaa agttgctagc
     4561 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     4621 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4681 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     4741 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4801 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4861 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4921 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4981 tggttcctgg ccaccgtcgg cgtgtcgccc gaccaccagg gcaagggtct gggcagcgcc
     5041 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5101 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5161 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccagca
     5221 cactggcggc cgttactagc ttctgcagca cgaccggttg ataatagata acttcgtata
     5281 gcatacatta tacgaagtta tgaattcgat atcaagctta tcgataatca acctctggat
     5341 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5401 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5461 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5521 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5581 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5641 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5701 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5761 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5821 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5881 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     5941 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6001 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6061 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6121 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     6181 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     6241 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     6301 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     6361 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     6421 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6481 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6541 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6601 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     6661 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6721 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6781 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6841 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6901 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7261 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7321 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7441 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7501 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7621 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7681 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     7741 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     7801 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     7861 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     7921 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     7981 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     8041 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     8101 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     8161 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     8221 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8281 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8341 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8401 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8461 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9301 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10621 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
    10681 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    10741 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
    10801 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
    10861 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    10921 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    10981 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    11041 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    11101 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    11161 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    11221 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
    11281 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
    11341 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
    11401 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
    11461 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    11521 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    11581 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    11641 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
    11701 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
    11761 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
    11821 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
    11881 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
    11941 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
    12001 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    12061 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    12121 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
    12181 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    12241 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    12301 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    12361 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    12421 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    12481 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    12541 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    12601 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    12661 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    12721 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    12781 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    12841 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    12901 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    12961 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    13021 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
    13081 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    13141 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    13201 tacagggaca gcagagatcc agtttggtta attaataact tcgtatagca tacattatac
    13261 gaagttatta aaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
    13321 ccttatttta acttgctatt tctagctcta aaacgctcac tgctccatcc tcccggtgtt
    13381 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
    13441 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
    13501 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattccaat
    13561 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
    13621 ctcttaatta attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc
    13681 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
    13741 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
    13801 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
    13861 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
    13921 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
    13981 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
    14041 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
    14101 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
    14161 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
    14221 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
    14281 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
    14341 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
    14401 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
    14461 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
    14521 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
    14581 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
    14641 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
    14701 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
    14761 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
    14821 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg
    14881 caggtcgact ctagagccac catg
//
